Molecular spectroscopic research Final technical report

Quadrupole rotation-vibration spectrum of hydrogen and predissociation rates of B state of iodin

Nomenclatural faux pas for Speyeria atlantis greyi Moeck, 1950 (Lepidoptera: Nymphalidae)

Nomenclatural errors associated with the nymphalid butterfly, Speyeria atlantis greyi Moeck, have persisted in the literature and electronic databases. We present here a synonymy of the various combinations and misspellings associated with it and clearly indicate the correct name and spelling based on Moecks (1950) original description. Additionally, color images of the holotype and allotype specimens are published herein for the first time

Sequence-Based Mapping and Genome Editing Reveal Mutations in Stickleback Hps5 Cause Oculocutaneous Albinism and the casper Phenotype.

Here, we present and characterize the spontaneous X-linked recessive mutation casper, which causes oculocutaneous albinism in threespine sticklebacks (Gasterosteus aculeatus). In humans, Hermansky-Pudlak syndrome results in pigmentation defects due to disrupted formation of the melanin-containing lysosomal-related organelle (LRO), the melanosome. casper mutants display not only reduced pigmentation of melanosomes in melanophores, but also reductions in the iridescent silver color from iridophores, while the yellow pigmentation from xanthophores appears unaffected. We mapped casper using high-throughput sequencing of genomic DNA from bulked casper mutants to a region of the stickleback X chromosome (chromosome 19) near the stickleback ortholog of Hermansky-Pudlak syndrome 5 (Hps5). casper mutants have an insertion of a single nucleotide in the sixth exon of Hps5, predicted to generate an early frameshift. Genome editing using CRISPR/Cas9 induced lesions in Hps5 and phenocopied the casper mutation. Injecting single or paired Hps5 guide RNAs revealed higher incidences of genomic deletions from paired guide RNAs compared to single gRNAs. Stickleback Hps5 provides a genetic system where a hemizygous locus in XY males and a diploid locus in XX females can be used to generate an easily scored visible phenotype, facilitating quantitative studies of different genome editing approaches. Lastly, we show the ability to better visualize patterns of fluorescent transgenic reporters in Hps5 mutant fish. Thus, Hps5 mutations present an opportunity to study pigmented LROs in the emerging stickleback model system, as well as a tool to aid in assaying genome editing and visualizing enhancer activity in transgenic fish

Trust with Private and Common Property: Effects of Stronger Property Right Entitlements

Is mutually beneficial cooperation in trust games more prevalent with private property or common property? Does the strength of property right entitlement affect the answer? Cox, Ostrom, Walker, et al. [1] report little difference between cooperation in private and common property trust games. We assign stronger property right entitlements by requiring subjects to meet a performance quota in a real effort task to earn their endowments. We find that cooperation is lower in common property trust games than in private property trust games, which is an idiosyncratic prediction of revealed altruism theory [2].

Revised time-of-flight calculations for high-latitude geomagnetic pulsations using a realistic magnetospheric magnetic field model

We present a simple time-of-flight analysis of Alfvén pulsations standing on closed terrestrial magnetic field lines. The technique employed in this study in order to calculate the characteristic period of such oscillations builds upon earlier time-of-flight estimates via the implementation of a more recent magnetospheric magnetic field model. In this case the model employed is the Tsyganenko (1996) field model, which includes realistic magnetospheric currents and the consequences of the partial penetration of the interplanetary magnetic field into the dayside magnetopause. By employing a simple description of magnetospheric plasma density, we are therefore able to estimate the period of standing Alfvén waves on geomagnetic field lines over a significantly wider range of latitudes and magnetic local times than in previous studies. Furthermore, we investigate the influence of changing season and upstream interplanetary conditions upon the period of such pulsations. Finally, the eigenfrequencies of magnetic field lines computed by the time-of-flight technique are compared with corresponding numerical solutions to the wave equation and experimentally observed pulsations on geomagnetic field lines

Quantitative Analysis of Electrotonic Structure and Membrane Properties of NMDA-Activated Lamprey Spinal Neurons

Parameter optimization methods were used to quantitatively analyze frequency-domain-voltage-clamp data of NMDA-activated lamprey spinal neurons simultaneously over a wide range of membrane potentials. A neuronal cable model was used to explicitly take into account receptors located on the dendritic trees. The driving point membrane admittance was measured from the cell soma in response to a Fourier synthesized point voltage clamp stimulus. The data were fitted to an equivalent cable model consisting of a single lumped soma compartment coupled resistively to a series of equal dendritic compartments. The model contains voltage-dependent NMDA sensitive (INMDA), slow potassium (IK), and leakage (IL) currents. Both the passive cable properties and the voltage dependence of ion channel kinetics were estimated, including the electrotonic structure of the cell, the steady-state gating characteristics, and the time constants for particular voltage- and time-dependent ionic conductances. An alternate kinetic formulation was developed that consisted of steady-state values for the gating parameters and their time constants at half-activation values as well as slopes of these parameters at half-activation. This procedure allowed independent restrictions on the magnitude and slope of both the steady-state gating variable and its associated time constant. Quantitative estimates of the voltage-dependent membrane ion conductances and their kinetic parameters were used to solve the nonlinear equations describing dynamic responses. The model accurately predicts current clamp responses and is consistent with experimentally measured TTX-resistant NMDA-induced patterned activity. In summary, an analysis method is developed that provides a pragmatic approach to quantitatively describe a nonlinear neuronal system

Analysis of on-orbit thermal characteristics of the 15-meter hoop/column antenna

In recent years, interest in large deployable space antennae has led to the development of the 15 meter hoop/column antenna. The thermal environment the antenna is expected to experience during orbit is examined and the temperature distributions leading to reflector surface distortion errors are determined. Two flight orientations corresponding to: (1) normal operation, and (2) use in a Shuttle-attached flight experiment are examined. A reduced element model was used to determine element temperatures at 16 orbit points for both flight orientations. The temperature ranged from a minimum of 188 K to a maximum of 326 K. Based on the element temperatures, orbit position leading to possible worst case surface distortions were determined, and the subsequent temperatures were used in a static finite element analysis to quantify surface control cord deflections. The predicted changes in the control cord lengths were in the submillimeter ranges

Lexical Decomposition, Silent Categories, and the Localizer Phrase

Linguistic