Extraction and Partial Characterization of Proteolytic Activities from the Cell Surface of Lactobacillus helveticus Zuc2

Abstract Proteolytic activities were extracted from a dairy Lactobacillus helveticus strain and partially characterized. A first cell envelope proteinase (CEP) was extracted using a high ionic strength buffer, both in the presence and in the absence of Ca 2+ . Moreover, cell treatment by 5 M LiCl allowed for the selective removal of the S-layer protein and CEP, suggesting an enzyme ionic linkage to the cell envelope similar to that observed for the Slayer structure. The enzyme specificity against α s1 -CN (f1–23) showed unusual activity on the Lys 3 -His 4 bond compared with other proteinases of the same species. A second proteinase appeared to be linked to the cell membrane because it was extractable only after membrane disgregation by detergents. Its specificity against CN fractions and α s1 -CN (f1–23) was different from that of the first CEP; moreover, the measured activity was lower than that of CEP

Energy Comparison between a Load Sensing System and Electro-Hydraulic Solutions Applied to a 9-Ton Excavator

With the increasingly stringent regulations on air quality and the consequent emission limits for internal combustion engines, researchers are concentrating on studying new solutions for improving efficiency and energy saving even in off-road mobile machines. To achieve this task, pump-controlled or displacement-controlled systems have inspired interest for applications in offroad working machines. Generally, these systems are derived from the union of a hydraulic machine coupled to an electric one to create compact components that could be installed near the actuator. The object of study of this work is a 9-ton excavator, whose hydraulic circuit is grounded on load sensing logic. The validated mathematical model, created previously in the Simcenter Amesim© environment, represents the starting point for developing electro-hydraulic solutions. Electric components have been inserted to create different architectures, both with open-and closed-circuit layouts, in order to compare the energy efficiency of the different configurations with respect to the traditional load sensing system. The simulations of a typical working cycle show the energy benefits of electrohydraulic solutions that allow for drastically reducing the mechanical energy required by the diesel engine and, consequently, the fuel consumption. This is mainly possible because of the elimination of directional valves and pressure compensators, which are necessary in a load sensing circuit, but are also a source of great energy dissipations. The results show that closed-circuit solutions produce the greatest benefits, with higher energy efficiencies than the open-circuit solution. Furthermore, closed-circuit configurations require fewer components, allowing for more compact and lighter solutions, as well as being cheaper

Extraordinary sensitivity of the electronic structure and properties of single-walled carbon nanotubes to molecular charge-transfer

Interaction of single-walled carbon nanotubes with electron donor and acceptor molecules causes significant changes in the electronic and Raman spectra, the relative proportion of the metallic species increasing on electron donation through molecular charge transfer, as also verified by electrical resistivity measurements.Comment: 15 pages, 5 figurre

An economic study of using phosphorus and lime in the Cerrado soils of Brazil

Neste trabalho foi analisado o uso de fósforo e calcário, nas culturas de milho e trigo de sequeiro em solos de cerrado do Brasil Central, tendo em vista duas situações: uma, de subsídio ao preço dos fatores, através das políticas especiais de crédito para a região; outra, na qual não se consideraram os subsídios. Foram determinadas funções de produção e taxas de retorno a investimentos nesses elementos, tendo por base experimentos realizados no Centro de Pesquisa Agropecuária dos Cerrados (CPAC).In this paper, the use of phosphorus and lime in corn and wheat production in the Cerrado region of Central Brazil was analysed by using production functions and internal rates of return in two price factor situations: a market price factor and a subsidized price factor. Experimental data was obtained from experiments conducted at the Centro de Pesquisa Agropecuária dos Cerrados (CPAC)