Persistent Vascular Collagen Accumulation Alters Hemodynamic Recovery from Chronic Hypoxia

Pulmonary arterial hypertension (PAH) is caused by narrowing and stiffening of the pulmonary arteries that increase pulmonary vascular impedance (PVZ). In particular, small arteries narrow and large arteries stiffen. Large pulmonary artery (PA) stiffness is the best current predictor of mortality from PAH. We have previously shown that collagen accumulation leads to extralobar PA stiffening at high strain (Ooi et al. 2010). We hypothesized that collagen accumulation would increase PVZ, including total pulmonary vascular resistance (Z0), characteristic impedance (ZC), pulse wave velocity (PWV) and index of global wave reflections (Pb/Pf), which contribute to increased right ventricular afterload. We tested this hypothesis by exposing mice unable to degrade type I collagen (Col1a1R/R) to 21 days of hypoxia (hypoxia), some of which were allowed to recover for 42 days (recovery). Littermate wild-type mice (Col1a1+/+) were used as controls. In response to hypoxia, mean PA pressure (mPAP) increased in both mouse genotypes with no changes in cardiac output (CO) or PA inner diameter (ID); as a consequence, Z0 (mPAP/CO) increased by ∼100% in both genotypes (pZC, PWV and Pb/Pf did not change. However, with recovery, ZC and PWV decreased in the Col1a1+/+ mice and remained unchanged in the Col1a1R/R mice. Z0 decreased with recovery in both genotypes. Microcomputed tomography measurements of large PAs did not show evidence of stiffness changes as a function of hypoxia exposure or genotype. We conclude that hypoxia-induced PA collagen accumulation does not affect the pulsatile components of pulmonary hemodynamics but that excessive collagen accumulation does prevent normal hemodynamic recovery, which may have important consequences for right ventricular function

Particle size distribution for copper heap leaching operations as established from 3D mineral exposure analysis by X-ray microCT

Journal ArticleThe particle size distribution (PSD) for copper heap leaching is an important consideration in the design and operation of any copper heap leaching facility. High-resolution 3D X-ray microtomography (XMT) can be used for the direct determination of the percentage of exposed valuable mineral grains in multiphase particles which vary in size from 40 mm down to a few hundred microns

Genética bioquímica del calamar argentino, Illex Argentinus

Previously reported results on natural populations of the Argentinean squid, Illex argentinus, urged further studies using electrophoretically detectable loci. The muscles of 202 adult squids caught in the Argentinean Sea were examined. Electrophoretic and staining methods for the 46 enzymes studied are given in detail. Forty-seven loci were detected (20 polymorphic), 45 of which are considered useable in population genetics screening. The expression of the loci coding for these enzymes is described and interpreted, and the presented results will serve as a basis for a more detailed examination of genetic variation in Argentinean squid populations.Estudios biológicos previos realizados en el calamar argentino, Illex argentinus, han sugerido la conveniencia de profundizar en el conocimiento de la estructura poblacional de la especie, utilizando el análisis genético de proteínas detectables electroforéticamente. Con esta finalidad se analizó el músculo esquelético de un total de 202 individuos capturados en el Mar Argentino. Se utilizó la técnica de electroforesis horizontal en gel de almidón al 11%. Se estudiaron 46 sistemas enzimáticos distintos. La expresión de los loci para estos enzimas son descritos e interpretados en detalle. Un total de 47 loci fueron detectados (20 polimórficos), de los cuales 45 se consideran utilizables para el análisis de la estructura poblacional de la especie

Potentialabhängige Morphologie von Kupferkatalysatoren während der Elektroreduktion von CO₂, ermittelt durch In‐situ‐Rasterkraftmikroskopie

Eine effiziente Charakterisierung der Katalysatoren im Realraum und unter realistischen Bedingungen der elektrochemischen CO2‐Reduktion (CO2RR) gelang durch elektrochemische AFM. Die Entwicklung von Strukturmerkmalen konnte von der Mikrometer‐ bis hin zur atomaren Skala aufgelöst werden. Auf einer Cu(100)‐Modelloberfläche treten während der CO2RR in 0,1 m KHCO3 ausgeprägte nanoskalige Oberflächenmorphologien auf, wobei sich granulare Strukturen potentialabhängig in glatt geschwungene Berg‐und‐Tal‐Oberflächen oder rechteckige Terrassenstrukturen umwandeln. Mit stärker negativem Potential steigt die Dichte der unterkoordinierten Cu‐Zentren während der CO2RR. Durch atomar aufgelöste In‐situ‐Bildgebung wird bei bestimmten kathodischen Potentialen spezifische Adsorption nachgewiesen, die die Katalysatorstruktur beeinflusst. Die Ergebnisse verdeutlichen die komplexen Abhängigkeiten zwischen Morphologie, Struktur, Defektdichte, angelegtem Potential und Elektrolyt bei Kupfer‐CO2RR‐Katalysatoren

Greening Rail Infrastructure for Carbon Benefits

© 2017 The Authors. Metropolitan Sydney has a network of rail corridors almost 400 kilometers in length, which vary in width from narrow cuttings to wide easements. With an appropriate selection of vegetation species, these corridors can be used to offset carbon emissions from railway operations. Simultaneously, the plantings will improve air quality, reduce pollution and storm water flows, ameliorate urban heating deliver biodiversity gains and improve urban design and property values. A pilot study was carried out on a representative section of one of the major rail lines in Sydney in 2016. A detailed inventory of vegetation on the selected site was obtained through a field survey and a variety of tools were used including i-Tree Eco to benchmark current carbon sequestration and storage (CS & S) levels. Study outcomes include the existing carbon capacity of the rail corridor's above-ground (and substrate) biomass and air pollution reduction. It also presents estimates of CS & S potential by identifying future planting areas within the pilot study corridor. These results are valuable for infrastructure policy formulation directed towards carbon emissions as well as securing the co-benefits noted above