Pasture Forage Quality in West Virginia - 1999 to 2001

WVU-Extension fact shee

Pasture Forage Quality in West Virginia

WVU-Extension fact shee

Influence of Sward Height, Concentrate Supplementation and Season on Grazing Activity of Beef Cows

The influence of sward height, concentrate supplementation and season on daily pattern of forage consumption of lactating beef cows grazing cool season pastures was determined. Cows (n=24; BW=535±10.8 kg) were randomly assigned to eight plots maintained at sward heights (SH) of either 4-8 cm or 8-12 cm and fed three levels of concentrate supplement: none = 0 kg/day, low = 3.12 kg/day or high = 6.24 kg/day. Cows on lower SH had greater (P \u3c .08) forage dry matter intake and spent an additional 1.2 hours/day (P \u3c .01) grazing compared to the higher SH. Cows on lower SH consumed 7.7 kg/day of forage dry matter and grazed 9.4 hours/day whereas those on higher SH consumed 7.1 kg/day and grazed 8.2 hours/day. Cows on lower SH grazed 0.7 hours/day (P \u3c .06) and 0.4 hours/day (P \u3c .08) longer at 06:00-10:00 hour and 11:00-13:00 hour, respectively, compared to the higher SH. Grazing efficiency (kg of forage consumed/hour of grazing) decreased (P \u3c .01) as season progressed. Season influenced duration of grazing activity (P \u3c .01). Cows grazed 0.5 hours longer (P \u3c .01) at 06:00-10:00 hour late in summer (August) compared to spring (May) and mid summer (June/July). Cows grazed 0.3 hours longer (P \u3c .08) at 11:00-13:00 hour during spring compared to late summer

Sward Height; Visual Estimate Compared with Plate Meter Height

Sward height (SH) is widely used in pasture research and farm practice to evaluate pasture conditions. Visual estimates can take less time than actual measurements. This study compared visually estimated SH, of continuously grazed cool-season permanent pasture with plate meter height. Weekly estimates, by five people, were made on 12, 0.75 to 1 ha fields, grazed at two intensities. Paired data (visual estimate and plate meter height) were subjected to variance and covariance analyses and prediction equations were developed. Average visual height accounted for 86% of the variability in plate meter height. Inclusion of other sources of variability in the model (treatment, date and interactions) accounted for only a further 8% of the variability in plate meter height. On the basis of the strong linear relationships found between visual and plate meter heights, a procedure combining both methods is proposed that would reduce by 25% the time required to make weekly SH measurements of 12, 0.75 to 1 ha fields

Comparison of Two Low-Input Cow/Calf Production Systems on Temperate Grassland

Two systems of grass farming were compared in an eight-year experiment in West Virginia, USA. The grassland consisted primarily of cocksfoot (Dactylis glomerara L.), Kentucky bluegrass (Poa pratensis L.), tall fescue (Festuca arundinacea Schreb.), and red (Trifolium pretense L.) and white clover (T. repens L.). The objective was to compare two systems of beef cow calf production. The experiment was a randomized complete block with two replicated treatments. The grassland of treatment 1 (system 1) was overseeded with legumes, grazing started 1 wk earlier and continued 1 wk later than treatment 2 (system 2) and calves were allowed to forward creep graze. The hay land of treatment 2 received 56 kg N ha-1, at the start of the growing season. Response was measured as calf weaning weight, hay production, and pre-grazing herbage accumulation. Each treatment/replicate (experimental unit) was assigned 6.5 ha divided into three grassland managements units: pasture, buffer and meadow. Pasture was grazed and not cut for hay. First growth of buffer and meadow was harvested as hay. Subsequently, buffer was grazed, meadow was again harvested, followed by late season grazing. Management units were divided into four paddocks. Animals occupied a paddock for 7 days resulting in 4-week grazing cycles from May to mid-November. Eight cow/calf pairs grazed each treatment/replicate (stocking rate 1.23 cow calves ha-1 ). Calves, born in March, were weaned in late September. System 1 calves gained 1.18 kg dy-1 (P \u3c 0.04 SE=0.01) compared with those on System 2 which gained 1.14 kg dy-1. Annual hay production on System 2 was 5784 kg ha-1, significantly more than on System 1 (P \u3c 0.01 SE=107). However, in System 1 extending the grazing season reduced the amount of hay required annually by 1680 kg ha-1. System 1 hay had a greater proportion of legume (9 vs. 3%, P \u3c 0.01 SE=0.5) and a lesser proportion of grass (75 vs. 85%, P \u3c 0.01 SE=0.7) than those of system 2

Recruitment collapse and population structure of the European eel shaped by local ocean current dynamics

Highlights: • We combine high-resolution ocean models with population genetics • Variation in wind-driven ocean currents mediates the collapse of A. anguilla • Female eels are philopatric within the Sargasso Sea, while males maintain gene flow • We present first evidence of the role of ocean currents in shaping species’ evolution Summary: Worldwide, exploited marine fish stocks are under threat of collapse [1]. Although the drivers behind such collapses are diverse, it is becoming evident that failure to consider evolutionary processes in fisheries management can have drastic consequences on a species’ long-term viability [2]. The European eel (Anguilla anguilla; Linnaeus, 1758) is no exception: not only does the steep decline in recruitment observed in the 1980s [ 3 and 4] remain largely unexplained, the punctual detection of genetic structure also raises questions regarding the existence of a single panmictic population [ 5, 6 and 7]. With its extended Transatlantic dispersal, pinpointing the role of ocean dynamics is crucial to understand both the population structure and the widespread decline of this species. Hence, we combined dispersal simulations using a half century of high-resolution ocean model data with population genetics tools. We show that regional atmospherically driven ocean current variations in the Sargasso Sea were the major driver of the onset of the sharp decline in eel recruitment in the beginning of the 1980s. The simulations combined with genotyping of natural coastal eel populations furthermore suggest that unexpected evidence of coastal genetic differentiation is consistent with cryptic female philopatric behavior within the Sargasso Sea. Such results demonstrate the key constraint of the variable oceanic environment on the European eel population

Increased prevalence of the pfdhfr/phdhps quintuple mutant and rapid emergence of pfdhps resistance mutations at codons 581 and 613 in Kisumu, Kenya

<p>Abstract</p> <p>Background</p> <p>Anti-malarial drug resistance in Kenya prompted two drug policy changes within a decade: sulphadoxine-pyrimethamine (SP) replaced chloroquine (CQ) as the first-line anti-malarial in 1998 and artemether-lumefantrine (AL) replaced SP in 2004. Two cross-sectional studies were conducted to monitor changes in the prevalence of molecular markers of drug resistance over the period in which SP was used as the first-line anti-malarial. The baseline study was carried out from 1999-2000, shortly after implementation of SP, and the follow-up study occurred from 2003-2005, during the transition to AL.</p> <p>Materials and methods</p> <p>Blood was collected from malaria smear-positive, symptomatic patients presenting to outpatient centers in Kisumu, Kenya, during the baseline and follow-up studies. Isolates were genotyped at codons associated with SP and CQ resistance. <it>In vitro </it>IC₅₀values for antifolates and quinolones were determined for isolates from the follow-up study.</p> <p>Results</p> <p>The prevalence of isolates containing the <it>pfdhfr </it>N51I/C59R/S108N/<it>pfdhps </it>A437G/K540E quintuple mutant associated with SP-resistance rose from 21% in the baseline study to 53% in the follow-up study (p < 0.001). Isolates containing the <it>pfdhfr </it>I164L mutation were absent from both studies. The <it>pfdhps </it>mutations A581G and A613S/T were absent from the baseline study but were present in 85% and 61%, respectively, of isolates from the follow-up study. At follow-up, parasites with mutations at five <it>pfdhps </it>codons, 436, 437, 540, 581, and 613, accounted for 39% of isolates. The CQ resistance-associated mutations <it>pfcrt </it>K76T and <it>pfmdr1 </it>N86Y rose from 82% to 97% (p = 0.001) and 44% to 76% (p < 0.001), respectively, from baseline to follow-up.</p> <p>Conclusions</p> <p>During the period in which SP was the first-line anti-malarial in Kenya, highly SP-resistant parasites emerged, including isolates harboring <it>pfdhps </it>mutations not previously observed there. SP continues to be widely used in Kenya; however, given the highly resistant genotypes observed in this study, its use as a first-line anti-malarial should be discouraged, particularly for populations without acquired immunity to malaria. The increase in the <it>pfcrt </it>K76T prevalence, despite efforts to reduce CQ use, suggests that either these efforts are not adequate to alleviate CQ pressure in Kisumu, or that drug pressure is derived from another source, such as the second-line anti-malarial amodiaquine.</p

Evaluation of the inion and asterion as neurosurgical landmarks for dural venous sinuses: osteological study on a sample of South African skull specimens

BACKGROUND : Sub-Saharan neurosurgeons most likely need to perform invasive procedures without the latest imaging and navigation technology in the operating room. Therefore, these surgeons need to utilize other methods such as superficial surface landmarks for neuro-navigation. Bony landmarks, including the inion and asterion, are commonly used during invasive procedures to pinpoint the location of the confluence of sinuses and transverse-sigmoid sinus junction, respectively. The purpose of this study was to investigate whether the inion and asterion can be used as superficial landmarks for the confluence of sinuses and the transverse-sigmoid sinus junction, respectively, in a South African population. METHODS : Fifty South African human skulls were used (25 male, 25 female). The micro-focus X-ray radiography and tomography facility (MIXRAD) at Necsa scanned and created three-dimensional virtual images of the skull specimens. Reference points were then inserted on the images and the relation between bony landmarks and venous sinuses was documented. RESULTS : The inion was directly related to the confluence of sinuses in 4% of the sample, whereas the asterion was directly related to the transverse-sigmoid sinus junction in 28% of the cases, on both the right and left sides. CONCLUSIONS : This study confirmed that neither the inion, nor the asterion, are directly related the confluence of sinuses and transverse-sigmoid sinus junction, respectively. These bony landmarks are more likely to be located either inferior, or not related at all, to the investigated dural venous sinuses.https://www.minervamedica.it/en/journals/neurosurgical-sciences2022-04-01hj2022Anatom

Clinical anatomy of the maxillary nerve block in pediatric patients

BACKGROUND : Anatomical landmarks in children are mostly extrapolated from studies in adults. Despite this, complex regional anesthetic procedures are frequently performed on pediatric patients. Sophisticated imaging techniques are available but the exact position, course and/or relationships of the structures are best understood with appropriate anatomical dissections. Maxillary nerve blocks are being used for peri-operative analgesia after cleft palate repair in infants. However, the best approach for blocking the maxillary nerve in pediatric patients has yet to be established. OBJECTIVE : To determine the best approach for blocking the maxillary nerve within the pterygopalatine fossa. METHODS : In an attempt to define an optimal approach for maxillary nerve block in this age group three approaches were simulated and compared on 10 dried pediatric skulls as well as 30 dissected pediatric cadavers. The needle course, including depth and angles, to block the maxillary nerve, as it exits the skull at the foramen rotundum within the pterygopalatine fossa, was measured and compared. Two groups were studied: Group 1 consisted of skulls and cadavers of neonates (0–28 days after birth) and Group 2 consisted of skulls and cadavers from 28 days to 1 year after birth. RESULTS : No statistically significant difference (P > 0.05) was found between the left and right side of each skull or cadaver. Only technique B, the suprazygomatic approach from the frontozygomatic angle towards the pterygopalatine fossa, exhibited no statistical significance (P > 0.05) when other measurements made on the skulls and cadavers were compared. Technique A, a suprazygomatic approach from the midpoint on the lateral border of the orbit, as well as technique C, an infrazygomatic approach with an entry at a point on a vertical line extending along the lateral orbit wall, showed statistical significant differences when measurements of the skulls and cadavers were compared. CONCLUSIONS : On the basis of these findings technique B produces the most consistent data for age groups 1 and 2 and supports the clinical findings recently reported.National Research Foundation (NRF)http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1460-95922015-07-30hb201