5,302 research outputs found
Design of an RSFQ Control Circuit to Observe MQC on an rf-SQUID
We believe that the best chance to observe macroscopic quantum coherence
(MQC) in a rf-SQUID qubit is to use on-chip RSFQ digital circuits for
preparing, evolving and reading out the qubit's quantum state. This approach
allows experiments to be conducted on a very short time scale (sub-nanosecond)
without the use of large bandwidth control lines that would couple
environmental degrees of freedom to the qubit thus contributing to its
decoherence. In this paper we present our design of a RSFQ digital control
circuit for demonstrating MQC in a rf-SQUID. We assess some of the key
practical issues in the circuit design including the achievement of the
necessary flux bias stability. We present an "active" isolation structure to be
used to increase coherence times. The structure decouples the SQUID from
external degrees of freedom, and then couples it to the output measurement
circuitry when required, all under the active control of RSFQ circuits.
Research supported in part by ARO grant # DAAG55-98-1-0367.Comment: 4 pages. More information and publications at
http://www.ece.rochester.edu:8080/users/sde/research/publications/index.htm
Temporal solitons in optical microresonators
Dissipative solitons can emerge in a wide variety of dissipative nonlinear
systems throughout the fields of optics, medicine or biology. Dissipative
solitons can also exist in Kerr-nonlinear optical resonators and rely on the
double balance between parametric gain and resonator loss on the one hand and
nonlinearity and diffraction or dispersion on the other hand. Mathematically
these solitons are solution to the Lugiato-Lefever equation and exist on top of
a continuous wave (cw) background. Here we report the observation of temporal
dissipative solitons in a high-Q optical microresonator. The solitons are
spontaneously generated when the pump laser is tuned through the effective zero
detuning point of a high-Q resonance, leading to an effective red-detuned
pumping. Red-detuned pumping marks a fundamentally new operating regime in
nonlinear microresonators. While usually unstablethis regime acquires unique
stability in the presence of solitons without any active feedback on the
system. The number of solitons in the resonator can be controlled via the pump
laser detuning and transitions to and between soliton states are associated
with discontinuous steps in the resonator transmission. Beyond enabling to
study soliton physics such as soliton crystals our observations open the route
towards compact, high repetition-rate femto-second sources, where the operating
wavelength is not bound to the availability of broadband laser gain media. The
single soliton states correspond in the frequency domain to low-noise optical
frequency combs with smooth spectral envelopes, critical to applications in
broadband spectroscopy, telecommunications, astronomy and low phase-noise
microwave generation.Comment: Includes Supplementary Informatio
HCF-1 amino- and carboxy-terminal subunit association through two separate sets of interaction modules: Involvement of fibronectin type 3 repeats
When herpes simplex virus infects permissive cells, the viral regulatory protein VP16 forms a specific complex with HCF-1, a preexisting nuclear protein involved in cell proliferation. The majority of HCF-1 in the cell is a complex of associated amino (BCF-1(N))- and carboxy (HCF-1(C))-terminal subunits that result from an unusual proteolytic processing of a large precursor polypeptide. Here, we have characterized the structure and function of sequences required for HCF-1(N) and HCF-1(C) subunit association. HCF-1 contains two matched pairs of self-association sequences called SAS1 and SAS2. One of these matched association sequences, SAS1, consists of a short 43-amino-acid region of the HCF-1(N) subunit, which associates with a carboxy-terminal region of the HCF-1(C) subunit that is composed of a tandem pair of fibronectin type 3 repeats, a structural motif known to promote protein-protein interactions. Unexpectedly, the related protein HCF-2, which is not proteolyzed, also contains a functional SAS1 association element, suggesting that this element does not function solely to maintain HCF-1(N) and HCF-1(C) subunit association. HCF-1(N) subunits do not possess a nuclear localization signal. We show that, owing to a carboxy-terminal HCF-1 nuclear localization signal, HCF-1(C) subunits can recruit HCF-1(N) subunits to the nucleus
MicroRNA-449a levels increase by several orders of magnitude during mucociliary differentiation of airway epithelia.
Improving Taxonomic Delimitation of Fungal Species in the Age of Genomics and Phenomics
Species concepts have long provided a source of debate among biologists. These lively debates have been important for reaching consensus on how to communicate across scientific disciplines and for advancing innovative strategies to study evolution, population biology, ecology, natural history, and disease epidemiology. Species concepts are also important for evaluating variability and diversity among communities, understanding biogeographical distributions, and identifying causal agents of disease across animal and plant hosts. While there have been many attempts to address the concept of species in the fungi, there are several concepts that have made taxonomic delimitation especially challenging. In this review we discuss these major challenges and describe methodological approaches that show promise for resolving ambiguity in fungal taxonomy by improving discrimination of genetic and functional traits. We highlight the relevance of eco-evolutionary theory used in conjunction with integrative taxonomy approaches to improve the understanding of interactions between environment, ecology, and evolution that give rise to distinct species boundaries. Beyond recent advances in genomic and phenomic methods, bioinformatics tools and modeling approaches enable researchers to test hypothesis and expand our knowledge of fungal biodiversity. Looking to the future, the pairing of integrative taxonomy approaches with multi-locus genomic sequencing and phenomic techniques, such as transcriptomics and proteomics, holds great potential to resolve many unknowns in fungal taxonomic classification
Improving Taxonomic Delimitation of Fungal Species in the Age of Genomics and Phenomics
Species concepts have long provided a source of debate among biologists. These lively debates have been important for reaching consensus on how to communicate across scientific disciplines and for advancing innovative strategies to study evolution, population biology, ecology, natural history, and disease epidemiology. Species concepts are also important for evaluating variability and diversity among communities, understanding biogeographical distributions, and identifying causal agents of disease across animal and plant hosts. While there have been many attempts to address the concept of species in the fungi, there are several concepts that have made taxonomic delimitation especially challenging. In this review we discuss these major challenges and describe methodological approaches that show promise for resolving ambiguity in fungal taxonomy by improving discrimination of genetic and functional traits. We highlight the relevance of eco-evolutionary theory used in conjunction with integrative taxonomy approaches to improve the understanding of interactions between environment, ecology, and evolution that give rise to distinct species boundaries. Beyond recent advances in genomic and phenomic methods, bioinformatics tools and modeling approaches enable researchers to test hypothesis and expand our knowledge of fungal biodiversity. Looking to the future, the pairing of integrative taxonomy approaches with multi-locus genomic sequencing and phenomic techniques, such as transcriptomics and proteomics, holds great potential to resolve many unknowns in fungal taxonomic classification
The Nature of the Nuclear H2O Masers of NGC 1068: Reverberation and Evidence for a Rotating Disk Geometry
We report new (1995) Very Large Array observations and (1984 - 1999)
Effelsberg 100m monitoring observations of the 22 GHz H2O maser spectrum of the
Seyfert 2 galaxy NGC 1068. The sensitive VLA observations provide a
registration of the 22 GHz continuum emission and the location of the maser
spots with an accuracy of ~ 5 mas. Within the monitoring data, we find evidence
that the nuclear masers vary coherently on time-scales of months to years, much
more rapidly than the dynamical time-scale. We argue that the nuclear masers
are responding in reverberation to a central power source, presumably the
central engine. Between October and November 1997, we detected a simultaneous
flare of the blue-shifted and red-shifted satellite maser lines. Reverberation
in a rotating disk naturally explains the simultaneous flaring. There is also
evidence that near-infrared emission from dust grains associated with the maser
disk also responds to the central engine. We present a model in which an X-ray
flare results in both the loss of maser signal in 1990 and the peak of the
near-infrared light curve in 1994. In support of a rotating disk geometry for
the nuclear masers, we find no evidence for centripetal accelerations of the
redshifted nuclear masers; the limits are +/- 0.006 km/s/year, implying that
the masers are located within 2 degrees of the kinematic line-of-nodes. We also
searched for high velocity maser emission like that observed in NGC 4258. In
both VLA and Effelsberg spectra, we detect no high velocity lines between +/-
350 km/s to +/- 850 km/s relative to systemic, arguing that masers only lie
outside a radius of ~ 0.6 pc (1.9 light years) from the central engine
(assuming a distance of 14.4 Mpc).Comment: 62 pages, 19 figure
Design and Testing of a Bionic Dancing Prosthesis
Traditionally, prosthetic leg research has focused on improving mobility for activities of daily living. Artistic expression such as dance, however, is not a common research topic and consequently prosthetic technology for dance has been severely limited for the disabled. This work focuses on investigating the ankle joint kinetics and kinematics during a Latin-American dance to provide unique motor options for disabled individuals beyond those of daily living. The objective of this study was to develop a control system for a bionic ankle prosthesis that outperforms conventional prostheses when dancing the rumba. The biomechanics of the ankle joint of a non-amputee, professional dancer were acquired for the development of the bionic control system. Subsequently, a professional dancer who received a traumatic transtibial amputation in April 2013 tested the bionic dance prosthesis and a conventional, passive prosthesis for comparison. The ability to provide similar torque-angle behavior of the biological ankle was assessed to quantify the biological realism of the prostheses. The bionic dancing prosthesis overlapped with 37 ± 6% of the non-amputee ankle torque and ankle angle data, compared to 26 ± 2% for the conventional, passive prosthesis, a statistically greater overlap (p = 0.01). This study lays the foundation for quantifying unique, expressive activity modes currently unavailable to individuals with disabilities. Future work will focus on an expansion of the methods and types of dance investigated in this work.Massachusetts Institute of Technology. Media Laborator
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