K0 form factor and charge radius in a covariant Salpeter model

The electromagnetic form factor for the $K^0$ is calculated in a covariant formulation of the Salpeter equation for $q\bar q$ - bound states, which has been presented recently for the mass spectrum, decay properties and form factors of the light pseudoscalar and vector mesons. The $K^0$ charge radius dependence on the difference between strange and down constituent quark mass is discussed.Comment: 5 pages including 3 uuencoded figures, RevTe

Instanton Effects in the Decay of Scalar Mesons

We show that instanton effects may play a crucial role in the decay of scalar mesons into two pseudoscalars. Particularly the branching ratios of two meson decays of the $f_0(1500)$, which is considered as a glue-ball candidate, are then compatible with an ordinary $q \bar{q}$-structure of this resonance and a small positive SU(3) mixing angle, close to a result recently calculated with the same instanton-induced force.Comment: 9 pages, uuencoded latex including two figure

Cytoskeletal stabilization of inhibitory interactions in immunologic synapses of mature human dendritic cells with natural killer cells

Human mature dendritic cells (DCs) can efficiently stimulate natural killer (NK)-cell responses without being targeted by their cytotoxicity. To understand this important regulatory crosstalk, we characterized the development of the immunologic synapse between mature DCs and resting NK cells. Conjugates between these 2 innate leukocyte populations formed rapidly, persisted for prolonged time periods and matured with DC-derived f-actin polymerization at the synapse. Polarization of IL-12 and IL-12R to the synapse coincided with f-actin polymerization, while other activating and inhibitory molecules were enriched at the interface between DCs and NK cells earlier. Functional assays revealed that inhibition of f-actin polymerization in mature synapses led to an increase of IFN-γ secretion and cytotoxicity by NK cells. This elevated NK-cell reactivity resulted from decreased inhibitory signaling in the absence of MHC class I polarization at the interface, which was observed on inhibition of f-actin polymerization in DCs. Thus, inhibitory signaling is stabilized by f-actin at the synapse between mature DCs and resting NK cells

TiAIN based nanoscale multilayer coatings designed to adapt their tribological properties at elevated temperatures

The addition of properly selected elements, coupled in nanoscale multilayer structures, can further enhance the properties of TiAlN coatings and bring new high performance. The incorporation of Y in the nanoscale pseudo-superlattice TiAlCrN/TiAlYN with typical period of 1.7 nm not only improves the oxidation resistance but also effectively reduces the coefficient of friction of the coating from 0.9 to 0.65 at temperatures in the range of 850–950 °C. The adaptation of the tribological properties occurs as a result of the preferential migration of the Y to the column boundaries. TiAlN/VN superlattice can achieve another self-adaptation process. During friction the coatings adapt themselves to the combined thermal and mechanical wear by the formation of highly lubricious vanadium-oxides due to high flash temperatures at the asperity contacts on the surface. The integrity of the bulk of the coating is retained, leading to exceptionally low, for superhard coatings, friction coefficient of 0.5 and a wear coefficient of 2 × 10−17 m3·N−1·m−1. The coatings have been deposited by the combined steered cathodic arc unbalanced magnetron sputtering method.</p

Kaon and Hyperon Form Factors in Kaon Electroproduction on the Nucleon

The electromagnetic form factors of strange mesons and baryons are studied by means of kaon electroproduction on the nucleon. The response functions that are sensitive to the K0, Lambda, Sigma, and KK*\gamma transition form factors are systematically explored. The effects of these form factors on several response functions are discussed.Comment: 10 pages, Latex2e, 12 postscript figures. Invited talk given at the International Conference on Hypernuclear and Strange Particle Physics (HYP97), Brookhaven National Laboratory, USA, October 13-18, 1997. To be published in Nucl. Phys.

Dynamics based alignment of proteins: an alternative approach to quantify dynamic similarity

<p>Abstract</p> <p>Background</p> <p>The dynamic motions of many proteins are central to their function. It therefore follows that the dynamic requirements of a protein are evolutionary constrained. In order to assess and quantify this, one needs to compare the dynamic motions of different proteins. Comparing the dynamics of distinct proteins may also provide insight into how protein motions are modified by variations in sequence and, consequently, by structure. The optimal way of comparing complex molecular motions is, however, far from trivial. The majority of comparative molecular dynamics studies performed to date relied upon prior sequence or structural alignment to define which residues were equivalent in 3-dimensional space.</p> <p>Results</p> <p>Here we discuss an alternative methodology for comparative molecular dynamics that does not require any prior alignment information. We show it is possible to align proteins based solely on their dynamics and that we can use these dynamics-based alignments to quantify the dynamic similarity of proteins. Our method was tested on 10 representative members of the PDZ domain family.</p> <p>Conclusions</p> <p>As a result of creating pair-wise dynamics-based alignments of PDZ domains, we have found evolutionarily conserved patterns in their backbone dynamics. The dynamic similarity of PDZ domains is highly correlated with their structural similarity as calculated with Dali. However, significant differences in their dynamics can be detected indicating that sequence has a more refined role to play in protein dynamics than just dictating the overall fold. We suggest that the method should be generally applicable.</p

Instantaneous Bethe-Salpeter equation: utmost analytic approach

The Bethe-Salpeter formalism in the instantaneous approximation for the interaction kernel entering into the Bethe-Salpeter equation represents a reasonable framework for the description of bound states within relativistic quantum field theory. In contrast to its further simplifications (like, for instance, the so-called reduced Salpeter equation), it allows also the consideration of bound states composed of "light" constituents. Every eigenvalue equation with solutions in some linear space may be (approximately) solved by conversion into an equivalent matrix eigenvalue problem. We demonstrate that the matrices arising in these representations of the instantaneous Bethe-Salpeter equation may be found, at least for a wide class of interactions, in an entirely algebraic manner. The advantages of having the involved matrices explicitly, i.e., not "contaminated" by errors induced by numerical computations, at one's disposal are obvious: problems like, for instance, questions of the stability of eigenvalues may be analyzed more rigorously; furthermore, for small matrix sizes the eigenvalues may even be calculated analytically.Comment: LaTeX, 23 pages, 2 figures, version to appear in Phys. Rev.

Monitoring Antigen Processing for MHC Presentation via Macroautophagy

Macroautophagy has recently emerged as an important catabolic process involved not only in innate immunity but also in adaptive immunity. Initially described to deliver intracellular antigens to MHC class II loading compartments, its molecular machinery has now also been described to impact the delivery of extracellular antigens to MHC class II loading compartments through the noncanonical use of the macroautophagy machinery during LC3-associated phagocytosis (LAP). Therefore, in pathological situations (viral or bacterial infections, tumorigenesis) the pathway might be involved in shaping CD4$^{+}$ T cell responses.In this chapter we describe three basic experiments for the monitoring and manipulation of macroautophagic antigen processing toward MHC class II presentation through the canonical pathway. Firstly, we will discuss how to monitor autophagic flux and autophagosome fusion with MHC class II loading compartments. Secondly, we will show how to target proteins to autophagosomes in order to monitor macroautophagy dependent antigen processing via their enhanced presentation on MHC class II molecules to CD4$^{+}$ T cells. And finally, we will describe how macroautophagy can be silenced in antigen presenting cells, like human monocyte-derived dendritic cells (DCs)

Electromagnetic Meson Form Factors in the Salpeter Model

We present a covariant scheme to calculate mesonic transitions in the framework of the Salpeter equation for $q\bar{q}$-states. The full Bethe Salpeter amplitudes are reconstructed from equal time amplitudes which were obtained in a previous paper\cite{Mue} by solving the Salpeter equation for a confining plus an instanton induced interaction. This method is applied to calculate electromagnetic form factors and decay widths of low lying pseudoscalar and vector mesons including predictions for CEBAF experiments. We also describe the momentum transfer dependence for the processes $\pi^0,\eta,\eta'\rightarrow\gamma\gamma^*$.Comment: 22 pages including 10 figure