Lipase Activity in Fermented Oil Seeds of Africa Locust Bean, (Parkia Biglobosa), Castor Seeds (Ricinu Communis) and African Oil Bean (Pentaclethra Macrophylla).

The activity of lipase in fermented Africa locust bean, Castor seed, and Africa oil bean was determined. The peak lipase activity for fermented Africa locust bean, Castor seed, and African oil bean were 13.3x10-3μmol/sec, 7.2 x10-3μmol/sec and 10.6 x10-3μmol/sec at day 5, 4 and 4 respectively. The optimum temperature and pH were 300C and 7.0 respectively for all the fermented seeds above. Increasing NaCl concentration decreases the activity of Lipase indicating that NaCl is an inhibitor of lipase. The effect of substrate concentration on lipase activity in the fermenting oil seeds shows the normal Michaelis-Menten curve. The Km for African locust bean is 0.065664mM, Castor seed is 0.067625mM, and African oil bean is 0.075848mM. While the Vmax are: 15.229x10-3 μmol/sec, 14.787x10-3μmol/sec and 13.184x10-3μmol/sec respectively. The findings of this work indicates that fermented African locust bean, Castor seed and Africa oil bean could be a source of lipase for industrial application. Key words: Fermentation, Lipase activity, Pentaclethra macrophylla, Daddaw

Analisa Pengaruh Servant Leadership Terhadap Job Performance Karyawan Di Hotel X Surabaya

- Analysis of the Influence of Servant Leadership on Employee Job Performance Hotel X Surabaya The study is intended to determine the influence of servant leadership on employee job performance at Hotel X Surabaya. Servant leadership dimensions used in this study are emotional healing, creating value for the community, conceptual skills, empowering, helping subordinate grow and succeed, putting subordinate first, behaving ethically.The analysis technique used is Partial Least Square (PLS). The result of this study show that the dimension of servant leadership, putting subordinate first has the most dominant influence on job performance. Moreover servant leadership has little impact on the job performance of the employees

Lupeol Acetate Isolated from n-Hexane Extract of Tapinanthus globiferus Leaf

A study was carried out to isolate and characterise Lupeol Acetate from the leaves of Tapinanthus globiferus. The dried pulverized plant material was extracted for 24 hours with n-hexane, dichloromethane, ethyl acetate and methanol solvents. The /n-hexane fraction obtained was subjected to column chromatography followed by preparative thin layer chromatography, which resulted in isolation of a colourless crystalline compound. The spectral data (1H NMR, 13C NMR and FTIR) and literature comparison infer that the isolated compound is a pentacyclic triterpenoid namely Lupeol Acetate.Keywords: Tapinanthus globiferus, Lupeol Acetate, Structure elucidatio

Application of Non-Model Dependent Hybrid Higher-Order Differential Feedback Controller on Crane System

Gantry Crane is a machine used for shipping of goods from one point to another. Speed, accuracy and safety are of paramount importance in gantry crane (GC) operation, but operating GC results in unwanted sway which degrades the accuracy and safety. In this paper, hybrid control schemes are proposed for precise trolley position control and sway suppression in GC systems. Output Based input shaping (OBIS) filter was designed using the output of the system for sway suppression and proportional integral derivative (PID), linear quadratic regulator (LQR), higher order differential feedback (HODF) controllers were incorporated separately for precise trolley position control. Based on Simulation studies and analysis, it was observed that LQR-OBIS controller shown more precise tracking and higher sway reduction control. But HODFC-OBIS is a model-free control schemes hence more robust

Successful multiple-dose methotrexate therapy for unruptured repeat ectopic pregnancy with high β-human chorionic gonadotropin value: a case report

Successfully managing an unruptured ectopic pregnancy necessitates prioritizing the preservation of fertility as the primary objective. Medical management is traditionally considered to be more successful at lower ß- human chorionic gonadotropin (HCG) values. However, there is emerging evidence that successful treatment can be achieved with modification of dosage regimen in the presence of high β-HCG value. We reported the successful management of a case of unruptured repeat ectopic pregnancy in a patient with high β-HCG. Mrs PA is a 25-year-old G4P0+3 with previous right salpingectomy due to ruptured ectopic gestation who presented with an ultrasound diagnosis of unruptured left tubal ectopic gestation at a gestational age of 6 weeks. The pre-treatment quantitative β-HCG level was 7066 IU/l. She had multiple dose methotrexate therapy which was well tolerated with normalization of β-HCG levels within 44 days. Hysterosalpingography done six (6) months post-treatment demonstrated patent left fallopian tube. She subsequently had spontaneous conception of an intrauterine pregnancy 16-months post-treatment. The pregnancy was carried to term and culminated in successful delivery at term. Multiple-dose chemotherapy was successful in this patient with high β-HCG level with no reported adverse effect

Effects of dietary supplementation of conjugated linoleic acids and their inclusion in semen extenders on bovine sperm quality

Isomers of conjugated linoleic acid (CLA) enhances circulating insulin-like growth factor I (IGF-I) levels. Furthermore, fertility rate of breeding bulls is positively correlated to seminal plasma IGF-I concentration. Our objective was to evaluate the effect of dietary CLA supplementa-tion and inclusion to the semen extender on bovine semen quality and freezability. Fourteen bulls, randomly assigned to control (CTL) and CLA (50 g/day) groups, were supplemented for 10 weeks. Samples were collected at Weeks −2 (before supplementation), 0, 4, 6 (during supplementation), 10, and 11 (after supplementation). Blood and seminal plasma were analyzed for IGF-I; the ejaculates were frozen in the following subgroups: CTL (no addition to semen extender), CLA c9, t11 (50 µM), CLA c9, t11 (100 µM), CLA t10, c12 (50 µM), CLA t10, c12 (100 µM), and CLA mix (50 µM each of CLA c9, t11 and CLA t10, c12). Sperm motility, morphology, viability, mitochondrial membrane potential, and reactive oxidative species were assessed. CLA supplementation decreased ejaculates’ total volume, increased sperm concentration, beat cross frequency, and decreased oxidative stress; it also increased plasma and seminal plasma IGF-I levels compared to the CTL. The inclusion of CLA c9, t11 100 µ M and CLA mixture in the extender increased live spermatozoa percentage post-thawing compared to other groups. Our results show a beneficial effect of CLA supplementation on semen quality; however, further studies evaluating fertilization rates are necessary to corroborate the results

Identification of regeneration-associated genes after central and peripheral nerve injury in the adult rat

Background: It is well known that neurons of the peripheral nervous system have the capacity to regenerate a severed axon leading to functional recovery, whereas neurons of the central nervous system do not regenerate successfully after injury. The underlying molecular programs initiated by axotomized peripheral and central nervous system neurons are not yet fully understood.Results: To gain insight into the molecular mechanisms underlying the process of regeneration in the nervous system, differential display polymerase chain reaction has been used to identify differentially expressed genes following axotomy of peripheral and central nerve fibers. For this purpose, axotomy induced changes of regenerating facial nucleus neurons, and non-regenerating red nucleus and Clarke's nucleus neurons have been analyzed in an intra-animal side-to-side comparison. One hundred and thirty five gene fragments have been isolated, of which 69 correspond to known genes encoding for a number of different functional classes of proteins such as transcription factors, signaling molecules, homeobox-genes, receptors and proteins involved in metabolism. Sixty gene fragments correspond to genomic mouse sequences without known function. In situ-hybridization has been used to confirm differential expression and to analyze the cellular localization of these gene fragments. Twenty one genes (similar to 15%) have been demonstrated to be differentially expressed.Conclusions: The detailed analysis of differentially expressed genes in different lesion paradigms provides new insights into the molecular mechanisms underlying the process of regeneration and may lead to the identification of genes which play key roles in functional repair of central nervous tissues