Mass production of Bacillus subtilis and Trichoderma viride for the control of Phyllosticta citricarpa (Teleomorph: Guignardia citricarpa).

The work was aimed at studying the production of cells and metabolites of Bacilius subtilis (ACB-69) and of Trichoderma viride conidia (ACB-14) on different substrates, since they can potentially control Phyllosticta citricarpa. Our results showed that the medium consisting of cotton meal added of hydrolized protein provided the highest yield of B. subtiiis cells (2.44 x IO9 cells/mL), after the culture had been incubated for three days. This liquid substrate also provided conditions for the bacterium to produce thermostable metabolites, in sufficient amounts to inhibit the plant pathogen's micelial growth. The production of B. subtilis under the solid fermentation system performed better on the brewers rice substrate; the number of bactéria; cells decreased as the substrate concentration increased. In general, the liquid medium yielded a higher amount of B. subtilis than the solid medium. With regard to the large scale production of T. viride, it was verified that the substrates tested had a low spore production; the best substrate among those tested (com cob + hydrolized protein) only yielded 2.17 x IO6 conidia/mL. O objetivo foi estudar a produção de células e de metabólitos de Bacillus subtilis (ACB-69) e de conídios de Trichoderma viride (ACB-14) em diferentes substratos, pois apresentam potencial para o controle de Phyllosticta citricarpa. O meio constituído de farelo de algodão acrescido de proteína hidrolisada foi o que proporcionou maior produção de células de B. subtilis (2,4 x 109 células/mL), após três dias de incubação da cultura. Esse substrato líquido também propiciou condições para que a bactéria produzisse metabólitos termoestáveis e, em quantidades suficientes para inibir o crescimento micelial do fitopatógeno. A produção de B. subtilis pelo sistema de fermentação sólida foi melhor no substrato quirera de arroz sendo que o número de células da bactéria diminuiu à medida que aumentou a concentração do substrato. De um modo geral, o meio líquido foi superior ao sólido para a produção de B. subtilis. Com relação à produção de T. viride, verificou-se que os substratos testados apresentaram baixa produção de esporos, sendo que o melhor substrato testado (sabugo de milho + proteína hidrolisada) produziu apenas 2,2 x 106 conídios/mL

Parental cooperation in an extreme hot environment: natural behaviour and experimental evidence

One of the most apparent examples of cooperation between unrelated individuals is biparental care whereby the male and the female parent share the rearing of the offspring. Theoretical models of care predict that selection should favour biparental care if it substantially improves the survival of the offspring. Although various ecological factors have been proposed to necessitate biparental care, experimental evidence is scant given the challenges of manipulating ecological factors in the natural habitat of animals. We carried out one such experiment in a small shorebird, the Kentish plover, Charadrius alexandrinus, that breeds in an extreme desert environment. Nest cover and thus exposure to solar radiation vary between nests, and we show that parents at exposed nests spent more time incubating than those at nests shaded by a bush (covered nests). Experimental removal and supplementation of nest cover gave results consistent with the observational data; at experimentally exposed nests both males and females increased incubation effort and relieved each other more frequently whereas at experimentally covered nests we observed the opposite. We conclude that exposure to extreme solar radiation influences biparental care and this necessitates parental cooperation in the Kentish plover. Furthermore, since parental care often coevolves with mating strategies, we conjecture that where the environment puts less pressure on the parents and provides the opportunity for reduced care, both mating systems and parental care can diversify over evolutionary time. (C) 2011 The Association for the Study of Animal Behaviour. Published by Elsevier Ltd. All rights reserved

Jeans Instability in a Tidally Disrupted Halo Satellite Galaxy

We use a hybrid test particle/N-body simulation to integrate 4 million massless test particle trajectories within a fully self-consistent 10^5 particle N-body simulation. The number of massless particles allows us to resolve fine structure in the spatial distribution and phase space of a dwarf galaxy as it is disrupted in the tidal field of a Milky Way type galaxy. The tidal tails exhibit nearly periodic clumping or a smoke-like appearance. By running simulations with different satellite particle mass, halo particle mass, number of massive and massless particles and with and without a galaxy disk, we have determined that the instabilities are not due to numerical noise, amplification of structure in the halo, or shocking as the satellite passes through the disk of the Galaxy. We measure Jeans wavelengths and growth timescales in the tidal tail and show that the Jeans instability is a viable explanation for the clumps. We find that the instability causes velocity perturbations of order 10 km/s. Clumps in tidal tails present in the Milky Way could be seen in stellar radial velocity surveys as well as number counts. We find that the unstable wavelength growth is sensitive to the simulated mass of dark matter halo particles. A simulation with a smoother halo exhibits colder and thinner tidal tails with more closely spaced clumps than a simulation with more massive dark matter halo particles. Heating by the halo particles increases the Jeans wavelength in the tidal tail affecting substructure development, suggesting an intricate connection between tidal tails and dark matter halo substructure.Comment: 15 pages, 7 figures, submitted to MNRAS, May 25 201

Qualitative and quantitative analysis of stability and instability dynamics of positive lattice solitons

We present a unified approach for qualitative and quantitative analysis of stability and instability dynamics of positive bright solitons in multi-dimensional focusing nonlinear media with a potential (lattice), which can be periodic, periodic with defects, quasiperiodic, single waveguide, etc. We show that when the soliton is unstable, the type of instability dynamic that develops depends on which of two stability conditions is violated. Specifically, violation of the slope condition leads to an amplitude instability, whereas violation of the spectral condition leads to a drift instability. We also present a quantitative approach that allows to predict the stability and instability strength

Validity of a multidimensional comprehensive psychosocial screening instrument based on the ESC cardiovascular prevention guidelines:Evidence from the general and cardiovascular patient population

Aim To evaluate the psychometric properties and validity of the updated version of the Dutch multidimensional Comprehensive Psychosocial Screening Instrument in patients with coronary heart disease and the general population, based upon guideline recommendations from the European Society for Cardiology. Method 678 participants (Mage = 48.2, SD = 16.8; 46% male) of the Dutch general population and 312 cardiac patients (Mage = 65.9, SD = 9.9; 77% male) who recently received percutaneous coronary intervention completed the Comprehensive Psychosocial Screening Instrument and validated questionnaires for depression (PHQ-9), anxiety (GAD-7), Type D personality (DS14), hostility (CMHS), anger (STAS-T), trauma (SRIP), and chronic work and family stress (ERI, MMQ-6). Results Confirmatory factor analysis (CFA) confirmed that the eight screened risk factors were best measured as separate entities, rather than broader indications of distress. Inter-instrument agreement, assessed with the intraclass coefficient (ICC) and the screening accuracy indicators (receiving operator characteristic [ROC] curves, sensitivity, specificity, and the positive and negative predictive values [PPV; NPV]) were good for most screened risk factors. PPV was low in low prevalence risk factors like anxiety, trauma, and depression. Conclusion Overall, the current version of the Comprehensive Psychosocial Screening Instrument has an acceptable performance in both populations, with a fair to excellent level of agreement with established full questionnaires. Besides a few suggestions for further refinement, the screener may be implemented in primary care and cardiological practice

A molecular insight into algal-oomycete warfare: cDNA analysis of <i>Ectocarpus siliculosus</i> infected with the basal oomycete <i>Eurychasma dicksonii</i>

Brown algae are the predominant primary producers in coastal habitats, and like land plants are subject to disease and parasitism. Eurychasma dicksonii is an abundant, and probably cosmopolitan, obligate biotrophic oomycete pathogen of marine brown algae. Oomycetes (or water moulds) are pathogenic or saprophytic non-photosynthetic Stramenopiles, mostly known for causing devastating agricultural and aquacultural diseases. Whilst molecular knowledge is restricted to crop pathogens, pathogenic oomycetes actually infect hosts from most eukaryotic lineages. Molecular evidence indicates that Eu. dicksonii belongs to the most early-branching oomycete clade known so far. Therefore Eu. dicksonii is of considerable interest due to its presumed environmental impact and phylogenetic position. Here we report the first large scale functional molecular data acquired on the most basal oomycete to date. 9873 unigenes, totalling over 3.5Mb of sequence data, were produced from Sanger-sequenced and pyrosequenced EST libraries of infected Ectocarpus siliculosus. 6787 unigenes (70%) were of algal origin, and 3086 (30%) oomycete origin. 57% of Eu. dicksonii sequences had no similarity to published sequence data, indicating that this dataset is largely unique. We were unable to positively identify sequences belonging to the RXLR and CRN groups of oomycete effectors identified in higher oomycetes, however we uncovered other unique pathogenicity factors. These included putative algal cell wall degrading enzymes, cell surface proteins, and cyclophilin-like proteins. A first look at the host response to infection has also revealed movement of the host nucleus to the site of infection as well as expression of genes responsible for strengthening the cell wall, and secretion of proteins such as protease inhibitors. We also found evidence of transcriptional reprogramming of E. siliculosus transposable elements and of a viral gene inserted in the host genome

Effect of tissue fit on corneal shape after transplantation

Postkeratoplasty astigmatism is now a major problem preventing visual recovery. Certain postopertive topographic characteristics are felt to be dictated by the fit of the donor corneal button in its recipient bed. Deficient tissue at the wound is predicted to contribute to the location of the steep meridian and excess tissue to the location of the flat meridian. In an eight-cat sample using our Fit Assessment Method and Photogrammetric Index Method, the authors tested the relationship between button fit in recipient bed and resulting corneal curvature at approximately 42, 161, and 289 postoperative days. Corneal symmetry improved between the first and second postoperative periods. Deficient tissue led to steepened curvature and ample tissue to flattened curvature in the first measurement period. When buttons fit poorly, deficient tissue led to steepness in the first postoperative period, but led to flattened curvature 90 deg away from the deficient tissue meridian in the second and third periods. The relationship between ample tissue and flattest postoperative curvature did not depend on the magnitude of button-bed disparity in any period. Corneal elasticity appeared to influence the way tissue disparity affected postoperative topography. Our findings support Troutman's balloon mode. When there was a large amount of uncompensated tissue disparity, the tissue deficiency exerted a force that shortened the translimbal chord. This produced both steepened curvature parallel to this chord soon after surgery and flattened curvature at 90 deg to the chord in the stable postoperative cornea