The Effects of Seasonal Variations In Chemistry and Hydrology on the Microbial Community and Its Sulfide Oxidation Potential In a Naturally Acidic Maine Stream

Sulfide minerals oxidize through interaction with water and oxygen, releasing hydrogen ions. The process often occurs naturally near metal sulfide deposits, and can be accelerated through mining. Microorganisms accelerate the rate of sulfide oxidation. Acidified streams typically contain high metal concentrations (e.g. aluminum) and microbes in these systems may develop resistances to metal toxicity. Stream flow can affect sulfide oxidation and microbial community structure. Baseflow can influence stream chemistry from interactions with the surrounding bedrock, while stormflow affects stream chemistry and the local microbial community through dilution and addition of microbes transported by runoff. Microbial community composition is affected by seasonal shifts in water chemistry and climate conditions like temperature and precipitation. Little work has addressed the effects of acidification upon the biogeochemistry in streams located near sulfide deposits in northern New England and similar ecosystems. Maine has numerous metallic sulfide deposits. It is important to understand what microbes are present in aqueous systems near these deposits, the effects of chemistry and climate on their structure, and their sulfide oxidation potential within acidifying conditions in case one of these deposits were ever to be mined. We conducted a field study to assess community structure and its relationships with changing seasonal chemistry and hydrology within a naturally acidic stream Blood Brook. In the field, Blood Brook chemistry and microbial community was sampled and studied across an 8-month period of seasonal transition. We found that the community was resembled a typical community, with a small population of circumneutral iron-oxidizing bacteria. Changes in the microbial community structure were primarily driven by changes in stream flow throughout the study period, with stormflow overall increasing diversity. A series of three, five-week, batch reactor experiments were also conducted to assess changes within the experimental community exposed to increased amounts of pyrite, and to assess how its sulfide oxidation potential changed from differing sampling conditions. Experiments were conducted using an abiotic and biotic treatment. During the experiments, we found no significant differences between abiotic and biotic sulfate concentration changes, but there were significant differences in pH changes between treatments. Microbial community analyses of the experimental solutions revealed that there were limited classified sulfide or iron oxidizing bacteria, despite precipitate evidence of circumneutral iron oxidizing bacteria. These data suggest that there is limited bacterial sulfide oxidation occurring, and that something else was driving biological pH changes. Dominant in the final communities were genera Acidocella and Acidisoma, indicating the acidic conditions drove the microbial community to become an acidophilic one. Precipitate observations revealed structures resembling those produced by previously identified circumneutral iron oxidized bacteria. The final experimental communities resembled those that have been observed in circumneutral iron-rich groundwater and surface water communities. We concluded that the Blood Brook microbial community is primarily a stream community, whose structure is primarily influenced in changes in hydrologic flow conditions. We also conclude that under experimental acidifying conditions, changes in the community are primarily driven by decreases in pH and increases in specific conductance

Pre-clearing vegetation of the coastal lowlands of the Wet Tropics Bioregion, North Queensland

A pre-clearing vegetation map and digital coverage at approximately 1:50 000 scale for the coastal lowlands (up to about 200 m elevation) of the Wet Tropics Bioregion, North Queensland is presented. The study area covers about 508 000 ha from Cooktown, 420 km south almost to Townsville (latitude 15° 30’–18° 20’ longitude 144° 50’–146° 40’). Data sources included historical aerial photography, early surveyors’ plans, explorers’ journals, previous vegetation maps, and maps of soils and geology. The pre-clearing mapping was built around the remnant vegetation mapping of Stanton & Stanton (2005), and the vegetation classification of this latter work was adopted. Vegetation units were further classified into regional ecosystems compatible with the standard State-wide system used by Queensland government. The digital coverage is part of the current Queensland Herbarium regional ecosystem coverage (Queensland Herbarium and Wet Tropics Management Authority 2005). Coloured maps (1:100 000 scale) of the pre-clearing vegetation of the Herbert, Tully, Innisfail and Macalister/Daintree subregions are on an accompanying CD-ROM. An evaluation of vegetation loss through clearing on the coastal lowlands of the Wet Tropics revealed several nearextinct vegetation communities and regional ecosystems, and many others that are drastically reduced in area. Even ecosystems occurring on poorly drained lands have suffered a surprisingly high level of loss due to the effectiveness of drainage operations. Grassland ecosystems were found to be widespread on the Herbert and Tully floodplains, but are now close to extinction. The lowlands vegetation of the Wet Tropics that remains today continues to be fragmented and degraded despite the introduction of State-wide broad-scale tree-clearing laws in 1999, and the cessation of broadscale tree-clearing in December 2006

Size dependence of solar X-ray flare properties

Non-thermal and thermal parameters of 85 solar flares of GOES class B1 to M6 (background subtracted classes A1 to M6) have been compared to each other. The hard X-ray flux has been measured by RHESSI and a spectral fitting provided flux and spectral index of the non-thermal emission, as well as temperature and emission measure of the thermal emission. The soft X-ray flux was taken from GOES measurements. We find a linear correlation in a double logarithmic plot between the non-thermal flux and the spectral index. The higher the acceleration rate of a flare, the harder the non-thermal electron distribution. The relation is similar to the one found by a comparison of the same parameters from several sub-peaks of a single flare. Thus small flares behave like small subpeaks of large flares. Thermal flare properties such as temperature, emission measure and the soft X-ray flux also correlate with peak non-thermal flux. A large non-thermal peak flux entails an enhancement in both thermal parameters. The relation between spectral index and the non-thermal flux is an intrinsic feature of the particle acceleration process, depending on flare size. This property affects the reported frequency distribution of flare energies.Comment: Astronomy and Astrophysics, in pres

Flare magnetic reconnection and relativistic particles in the 2003 October 28 event

An X17.2 solar flare occurred on 2003 October 28, accompanied by multi-wavelength emissions and a high flux of relativistic particles observed at 1AU. We present the analytic results of the TRACE, SOHO, RHESSI, ACE, GOES, hard X-ray (INTEGRAL satellite), radio (Onderejov radio telescope), and neutron monitor data. It is found that the inferred magnetic reconnection electric field correlates well with the hard X-ray, gamma-ray, and neutron emission at the Sun. Thus the flare's magnetic reconnection probably makes a crucial contribution to the prompt relativistic particles, which could be detected at 1 AU. Since the neutrons were emitted a few minutes before the injection of protons and electrons, we propose a magnetic-field evolution configuration to explain this delay. We do not exclude the effect of CME-driven shock, which probably plays an important role in the delayed gradual phase of solar energetic particles.Comment: 5 pages, 7 figures, accepted by A&

Do Solar Coronal Holes Affect the Properties of Solar Energetic Particle Events?

The intensities and timescales of gradual solar energetic particle (SEP) events at 1 AU may depend not only on the characteristics of shocks driven by coronal mass ejections (CMEs), but also on large-scale coronal and interplanetary structures. It has long been suspected that the presence of coronal holes (CHs) near the CMEs or near the 1-AU magnetic footpoints may be an important factor in SEP events. We used a group of 41 E (is) approx. 20 MeV SEP events with origins near the solar central meridian to search for such effects. First we investigated whether the presence of a CH directly between the sources of the CME and of the magnetic connection at 1 AU is an important factor. Then we searched for variations of the SEP events among different solar wind (SW) stream types: slow, fast, and transient. Finally, we considered the separations between CME sources and CH footpoint connections from 1 AU determined from four-day forecast maps based on Mount Wilson Observatory and the National Solar Observatory synoptic magnetic-field maps and the Wang-Sheeley-Arge model of SW propagation. The observed in-situ magnetic-field polarities and SW speeds at SEP event onsets tested the forecast accuracies employed to select the best SEP/CH connection events for that analysis. Within our limited sample and the three analytical treatments, we found no statistical evidence for an effect of CHs on SEP event peak intensities, onset times, or rise times. The only exception is a possible enhancement of SEP peak intensities in magnetic clouds

A synoptic view of solar transient evolution in the inner heliosphere using the Heliospheric Imagers on STEREO

By exploiting data from the STEREO/heliospheric imagers (HI) we extend a well-established technique developed for coronal analysis by producing time-elongation plots that reveal the nature of solar transient activity over a far more extensive region of the heliosphere than previously possible from coronagraph images. Despite the simplicity of these plots, their power in demonstrating how the plethora of ascending coronal features observed near the Sun evolve as they move antisunward is obvious. The time-elongation profile of a transient tracked by HI can, moreover, be used to establish its angle out of the plane-of-the-sky; an illustration of such analysis reveals coronal mass ejection material that can be clearly observed propagating out to distances beyond 1AU. This work confirms the value of the time-elongation format in identifying/characterising transient activity in the inner heliosphere, whilst also validating the ability of HI to continuously monitor solar ejecta out to and beyond 1A

Solar flares with and without SOHO/LASCO coronal mass ejections and type II shocks

We analyse of a set of radio rich (accompanied by type IV or II bursts) solar flares and their association with SOHO/LASCO Coronal Mass Ejections in the period 1998 2000. The intensity, impulsiveness and energetics of these events are investigated. We find that, on the average, flares associated both with type IIs and CMEs are more impulsive and more energetic than flares associated with type IIs only (without CME reported), as well as flares accompanied by type IV continua but not type II shocks. From the last two classes, flares with type II bursts (without CMEs reported) are the shortest in duration and the most impulsive.Comment: Advances in Space Research, Volume 38, Issue 5, p. 1007-101

Effects of Exogenous Antioxidants on Dietary Iron Overload

In dietary iron overload, excess hepatic iron promotes liver damage. The aim was to attenuate free radical-induced liver damage using vitamins. Four groups of 60 Wistar rats were studied: group 1 (control) was fed normal diet, group 2 (Fe) 2.5% pentacarbonyl iron (CI) followed by 0.5% Ferrocene, group 3 (Fe + V gp) CI, Ferrocene, plus vitamins A and E (42× and 10× RDA, respectively), group 4 (Fe – V gp) CI, Ferrocene diet, minus vitamins A and E. At 20 months, glutathione peroxidase (GPx), superoxide dismutase (SOD), Oxygen Radical Absorbance Capacity (ORAC), Ames mutagenicity test, AST, ALT and 4-hydroxynonenal (4-HNE) immunohistochemistry were measured. 8OHdG levels of the Fe + V and Fe – V groups were 346 ± 117 and 455 ± 151, ng/g w.wt, respectively. Fe + V and Fe – V differences were significant (p<0.005). A positive correlation between DNA damage and mutagenesis existed (p<0.005) within the iron-fed gps. AST levels for Fe + V and Fe – V groups were 134.6 ± 48.6 IU and 202.2 ± 50.5 IU, respectively. Similarly, ALT levels were 234.6 ± 48.3 IU and 329.0 ± 48.6 IU, respectively. However, Fe – V and Fe + V groups transaminases were statistically insignificant. 4-HNE was detected in Fe + V and Fe – V gp livers. Vitamins A and E could not prevent hepatic damage