4,264 research outputs found
Het beschrijven van paleoceanografische variabiliteit in het zuiden van de Golf van Cadiz tijdens de laatste glaciale cyclus, een multi-proxie aanpak
Large magnetoresistance using hybrid spin filter devices
A magnetic "spin filter" tunnel barrier, sandwiched between a non-magnetic
metal and a magnetic metal, is used to create a new magnetoresistive tunnel
device, somewhat analogous to an optical polarizer-analyzer configuration. The
resistance of these trilayer structures depends on the relative magnetization
orientation of the spin filter and the ferromagnetic electrode. The spin
filtering in this configuration yields a previously unobserved
magnetoresistance effect, exceeding 100%.Comment: 3.5 pages, 3 figures, submitted to Appl. Phys. Let
Endoscopic treatment of obesity and metabolic disorders : basic and clinical studies on the effect of the Duodenal-Jejunal bypass liner
Public exhibit for demonstrating the quantum of electrical conductance
We present a new robust setup that explains and demonstrates the quantum of
electrical conductance for a general audience and which is continuously
available in a public space. The setup allows users to manually thin a gold
wire of several atoms in diameter while monitoring its conductance in real
time. During the experiment, a characteristic step-like conductance decrease
due to rearrangements of atoms in the cross-section of the wire is observed.
Just before the wire breaks, a contact consisting of a single atom with a
characteristic conductance close to the quantum of conductance can be
maintained up to several seconds. The setup is operated full-time, needs
practically no maintenance and is used on different educational levels
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Whole-Cell Analysis of Low-Density Lipoprotein Uptake by Macrophages Using STEM Tomography
Nanoparticles of heavy materials such as gold can be used as markers in quantitative electron microscopic studies of protein distributions in cells with nanometer spatial resolution. Studying nanoparticles within the context of cells is also relevant for nanotoxicological research. Here, we report a method to quantify the locations and the number of nanoparticles, and of clusters of nanoparticles inside whole eukaryotic cells in three dimensions using scanning transmission electron microscopy (STEM) tomography. Whole-mount fixed cellular samples were prepared, avoiding sectioning or slicing. The level of membrane staining was kept much lower than is common practice in transmission electron microscopy (TEM), such that the nanoparticles could be detected throughout the entire cellular thickness. Tilt-series were recorded with a limited tilt-range of 80° thereby preventing excessive beam broadening occurring at higher tilt angles. The 3D locations of the nanoparticles were nevertheless determined with high precision using computation. The obtained information differed from that obtained with conventional TEM tomography data since the nanoparticles were highlighted while only faint contrast was obtained on the cellular material. Similar as in fluorescence microscopy, a particular set of labels can be studied. This method was applied to study the fate of sequentially up-taken low-density lipoprotein (LDL) conjugated to gold nanoparticles in macrophages. Analysis of a 3D reconstruction revealed that newly up-taken LDL-gold was delivered to lysosomes containing previously up-taken LDL-gold thereby forming onion-like clusters
Whole-cell analysis of low-density lipoprotein uptake by macrophages using STEM tomography
Nanoparticles of heavy materials such as gold can be used as markers in quantitative electron microscopic studies of protein distributions in cells with nanometer spatial resolution. Studying nanoparticles within the context of cells is also relevant for nanotoxicological research. Here, we report a method to quantify the locations and the number of nanoparticles, and of clusters of nanoparticles inside whole eukaryotic cells in three dimensions using scanning transmission electron microscopy (STEM) tomography. Whole-mount fixed cellular samples were prepared, avoiding sectioning or slicing. The level of membrane staining was kept much lower than is common practice in transmission electron microscopy (TEM), such that the nanoparticles could be detected throughout the entire cellular thickness. Tilt-series were recorded with a limited tilt-range of 80u thereby preventing excessive beam broadening occurring at higher tilt angles. The 3D locations of the nanoparticles were nevertheless determined with high precision using computation. The obtained information differed from that obtained with conventional TEM tomography data since the nanoparticles were highlighted while only faint contrast was obtained on the cellular material. Similar as in fluorescence microscopy, a particular set of labels can be studied. This method was applied to study the fate of sequentially up-taken low-density lipoprotein (LDL) conjugated to gold nanoparticles in macrophages. Analysis of a 3D reconstruction revealed that newly up-taken LDL-gold was delivered to lysosomes containing previously up-taken LDL-gold thereby forming onion-like clusters
Quasi-one-dimensional behavior of (CH3)2NH2MnCl3 (DMMC)
The heat capacity of dimethyl ammonium manganese trichloride has been investigated for 1.6<T<50 K. A transition to a three-dimensional antiferromagnetically ordered state has been observed at 3.60 K, which is supported by nuclear-magnetic-resonance and susceptibility measurements. The critical entropy did amount to 3.4%. The magnetic heat capacity in the paramagnetic region could be described very well by a S=52 Heisenberg linear chain system with Jk=-5.8±0.7 K. The data for kT|J|<1.5, together with the earlier data on (CH3)4NMnCl3, corroborate the suggested low-temperature behavior of such a system.</p
Dionysius and Longinus on the Sublime: Rhetoric and Religious Language
Classics and Classical Civilizatio
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