Mathematical simulation of power conditioning systems. Volume 1: Simulation of elementary units. Report on simulation methodology

Methods and algorithms used for the simulation of elementary power conditioning units buck, boost, and buck-boost, as well as shunt PWM are described. Definitions are given of similar converters and reduced parameters. The various parts of the simulation to be carried out are dealt with; local stability, corrective network, measurements of input-output impedance and global stability. A simulation example is given

Traveling wave tube development for a serrodyne re-entrant amplifier

Traveling wave tube development for serrodyne re-entrant amplifie

Indoor radon survey in university buildings: a case study of Sapienza - University of Rome

The indoor radon concentration in underground workplaces pertaining to Sapienza – University of Rome have been monitored since the 90’s according to prescription of Italian Legislative Decree 230/95. In the last years, the recommendations contained in the Council Directive 2013/59/Euratom have shifted the focus to all indoor exposure situations by promoting actions to identify workplaces and dwellings with radon concentrations exceeding the reference level of 300 Bq/m3. In response to the upcoming transposition into national legislation, Sapienza has promoted the first Italian survey addressing workplaces in university buildings, regardless of the position with respect to the ground floor. The survey has interested more than three hundred workplaces, i.e. administration and professors’ offices, research and educational laboratories, conference rooms and classrooms, distributed in fifteen different buildings. Places monitored are strongly heterogeneous in terms of users’ habit, occupancy pattern and building characteristics. The influence of these parameters into seasonal variation have been addressed by organizing the survey in four quarters. The indoor radon concentration is measured by solid state nuclear track detectors, CR39. The aim of the paper is to present features, methods and intermediate results of the survey. The work, relying on the analysis of previous measurements interesting underground workplaces, focuses on methodology followed during all the preliminary and preparatory phases: active measurements by ionization chamber radon continuous monitor, radon progeny equilibrium factor estimations by radon daughters monitor, strategies for occupants’ awareness, positioning protocol and provisions to maximize representativity of results

Parent Interaction In Primetime Family Themed Television Portrayals: A Replication And Extension Of Dail And Way\u27s (1985) Content Analysis

This research is a replication and extension of Dail and Way’s (1985) content analysis identifying parent interactions portrayed in family oriented prime time network television programs. Family structure, parent role, child rearing, and child responses were coded from five episodes each of eight different programs from 2014-2015 television season. The programs presented parent roles more often than child rearing, while mothers were found in child rearing more often than fathers. Traditional family structures were most prevalent with fewer single parent households and a new presence of same-sex parents. Mothers and fathers were still portrayed stereotypically, but children’s responses were more realistic and further reinforced traditional gender roles. Child responses reinforced traditional and rejected non-traditional parent interactions according to the parents’ sex, partnership, and occupation

Detection and identification of Phytophthora species in southern Italy by RFLP and sequence analysis of PCR-amplified nuclear ribosomal DNA.

In four neighbouring regions of southern Italy, Basilicata, Campania, Apulia and Calabria, pepper and zucchini plants showing Phytophthora blight symptoms, tomato plants with either late blight or buckeye rot symptoms, plants of strawberry showing crown rot symptoms and declining clementine trees with root and fruit rot were examined for Phytophthora infections by means of polymerase chain reaction (PCR) assays, using primers directed to nuclear ribosomal DNA (rDNA) repeat sequences. All diseased plants and trees examined tested positive. The detected fungal-like organisms were differentiated and characterized on the basis of primer specificity as well as through extensive restriction fragment length polymorphism (RFLP) and sequence analysis of PCR-amplified rDNA. Phytophthora capsici was identified in diseased pepper and zucchini plants, P. infestans was identified in tomato with late blight symptoms whereas buckeye rot-affected tomatoes and diseased strawberry plants proved to be infected by P. nicotianae and P. cactorum, respectively. Declining clementine trees were infected with P. citrophthora and P. nicotianae in about the same proportion. Also, thirty-one pure culture-maintained isolates of Phytophthora which had previously been identified in southern Italy by traditional methods but were never examined molecularly, were examined by RFLP and sequence analysis of PCR-amplified nuclear rDNA. Among these, an isolate from gerbera which had previously been identified by traditional methods only at genus level, was assigned to P. tentaculata. For the remaining pure culture-maintained isolates examined, the molecular identification data obtained corresponded with those delineated by traditional methods. Most of the diseases examined were already known to occur in southern Italy but the pathogens were molecularly detected and fully characterized at nuclear rDNA repeat level only from other geographic areas, very often outside Italy. A new disease to southern Italy was the Phytophthora blight of zucchini. This is also the first report on the presence and molecular identification of P. tentaculata from Italy

Automatic interpretation of oblique ionograms

We present an algorithm for the identification of trace characteristics of oblique ionograms allowing determination of the Maximum Usable Frequency (MUF) for communication between the transmitter and receiver. The algorithm automatically detects and rejects poor quality ionograms. We performed an exploratory test of the algorithm using data from a campaign of oblique soundings between Rome, Italy (41.90 N, 12.48 E) and Chania, Greece (35.51 N, 24.01 E) and also between Kalkarindji, Australia (17.43 S, 130.81 E) and Culgoora, Australia (30.30 S, 149.55 E). The success of these tests demonstrates the applicability of the method to ionograms recorded by different ionosondes in various helio and geophysical conditions

Scientific review on the ionospheric absorption and research prospects of a Complex Eikonal model for one-layer ionosphere

Thepresent paper conducts a scientific review on ionospheric absorption, extrapolating the research prospects of a complex eikonal model for one-layer ionosphere. As regards the scientific review, here a quasi-longitudinal (QL) approximation for nondeviative absorption is deduced which is more refined than the corresponding equation reported by Davies (1990). As regards the research prospects, a complex eikonal model for one-layer ionosphere is analyzed in depth here, already discussed by Settimi et al. (2013). A simple formula is deduced for a simplified problem. A flat, layered ionospheric medium is considered, without any horizontal gradient. The authors prove that the QL nondeviative amplitude absorption according to the complex eikonal model is more accurate than Rawer’s theory (1976) in the range of middle critical frequencies

Detection of ochratoxin a using molecular beacons and real-time PCR thermal cycler

We developed a simple and cheap assay for quantitatively detecting ochratoxin A (OTA) in wine. A DNA aptamer available in literature was used as recognition probe in its molecular beacon form, i.e., with a fluorescence-quenching pair at the stem ends. Our aptabeacon could adopt a conformation allowing OTA binding, causing a fluorescence rise due to the increased distance between fluorophore and quencher. We used real-time PCR equipment for capturing the signal. With this assay, under optimized conditions, the entire process can be completed within 1 h. In addition, the proposed system exhibited a good selectivity for OTA against other mycotoxins (ochratoxin B and aflatoxin M1) and limited interference from aflatoxin B1 and patulin. A wide linear detection range (0.2–2000 μM) was achieved, with LOD = 13 nM, r = 0.9952, and R2 = 0.9904. The aptabeacon was also applied to detect OTA in red wine spiked with the same dilution series. A linear correlation with a LOD = 19 nM, r = 0.9843, and R2 = 0.9708 was observed, with recoveries in the range 63%–105%. Intra- and inter-day assays confirmed its reproducibility. The proposed biosensor, although still being finalized, might significantly facilitate the quantitative detection of OTA in wine samples, thus improving their quality control from a food safety perspective

Sub-lethal 5-fluorouracil dose challenges planarian stem cells promoting transcriptional profile changes in the pluripotent sigma-class neoblasts

Under physiological conditions, the complex planarian neoblast system is a composite of hierarchically organized stem cell sub-populations with sigma-class neoblasts, including clonogenic neoblasts, endowed with larger self-renewal and differentiation capabilities, thus generating all the other sub-populations and dominating the regenerative process. This complex system responds to differentiated tissue demands, ensuring a continuous cell turnover in a way to replace aged specialized cells and maintain tissue functionality. Potency of the neoblast system can be appreciated under challenging conditions in which these stem cells are massively depleted and the few remaining repopulate the entire body, ensuring animal resilience. These challenging conditions offer the possibility to deepen the relationships among different neoblast sub-populations, allowing to expose uncanonical properties that are negligible under physiological conditions. In this paper, we employ short, sub-lethal 5-fluorouracil treatment to specifically affect proliferating cells passing through the S phase and demonstrate that S-phase slowdown triggers a shift in the transcriptional profile of sigma neoblasts, which reduces the expression of their hallmark sox-P1. Later, some cells reactivate sox-P1 expression, suggesting that some neoblasts in the earlier steps of commitment could modulate their expression profile, reacquiring a wider differentiative potential