Can you transform a country by design?

At the beginning of 1993 an enthusiastic designer from London considered that the impossible might be possible, to help reverse the downward spiral of the economy of Romania through product design. Groups of British and Romanian designers are collaborating on various design projects that can improve the living standards of the average Romanian by providing work, education and affordable products. Although it is too early to say if the scheme has been a success, already it has progressed further than many could have hoped. This paper describes the stages towards fulfilling this dream

A novel microfluidic enrichment technique for carbonylated proteins

Proteins are the building blocks of cells in living organisms, and are composed of amino acids. The expression of proteins is regulated by the processes of transcription and translation. Proteins undergo post-translational modifications in order to dictate their role physiologically within a cell. Not all post-translational modifications are beneficial for the protein or the cell. One type of post-translational modification, called carbonylation, irreversibly places a carbonyl group onto an amino acid residue, most commonly proline, lysine, arginine, and threonine. This modification can have severe consequences physiologically, including loss of solubility, loss of function, and protein aggregation. Carbonylated proteins have commonly been used as a marker of oxidative stress. Oxidative stress has been suggested to play a role in many human disease states, such as Alzheimer\u27s Disease, Amyotrophic Lateral Sclerosis, Parkinson\u27s Disease, inflammatory diseases, and others. Evidence shows oxidative stress to be a contributing factor in the progression of aging. Therefore, markers of oxidative stress, such as carbonylated proteins, can provide key information for the development of valuable therapeutics for these conditions. However, they are found in low abundance in samples and require enrichment prior to proteomic-based studies. Currently, affinity chromatography is the chosen method for enriching carbonylated proteins in a sample. However, the technique has significant drawbacks, including a large sample requirement, a large time requirement, the need for derivatization, and a high dilution of the sample post elution. This dissertation introduces a microfluidic enrichment technique for carbonylated proteins. The technique involves the surface modification of a polymer microchip for selective capture of carbonylated proteins. The surface chemistry is verified using different analytical techniques. Specificity of the target molecule\u27s capture is demonstrated using a native protein. The capture conditions are optimized experimentally by studying four unique variables. Lastly, theoretical modeling is performed to determine the conditions that would lead to the technique\u27s failure. It is seen that the technique can selectively capture target proteins from a flowing solution, even in the presence of an unoxidized protein. Protein capture is most dependent upon flow rate and crosslinker concentration. The flow rates required to break the bonds formed between an oxidized protein and the crosslinker exceeds feasible levels within a microfluidic channel. The microfluidic enrichment technique provides a promising alternative to the current gold standard of avidin affinity chromatography. The device has promise as a possible protein biomarker discovery tool in the search for therapeutic targets in human disease states where oxidative stress has been implicated

Measurement equivalence of the Birth Satisfaction Scale-Revised (BSS-R): further evidence of construct validity

Objective and background The 10-item Birth Satisfaction Scale-Revised (BSS-R) is being increasingly used internationally, including the development of translated versions of the tool. However, to date, a direct comparison between the original version of the tool and a non-English language translated version has yet to be conducted. Recognising that measurement equivalence is critical in order to be able to meaningfully compare scores on the measure between different versions, the current study sought to evaluate the measurement invariance characteristics of the BSS-R within this context. Methods: A secondary analysis of two data sets. The study used a measurement invariance testing approach to determine the relative equivalence between the original UK English-language version and the Greek-translated version of the BSS-R. Participants were a convenience sample of UK (n = 228) and Greek (n = 162) postnatal women. Results: The BSS-R was found to offer an excellent model fit with pooled data, a robust configural model and metric-level invariance between English- and Greek-language versions. The BSS-R was also found to demonstrate partial scalar invariance, with 80% of item intercepts non-invariant between both versions. Two non-invariant items at the scalar level are likely to represent real differences between participant groups in terms of birth satisfaction as an artefact of service delivery type and relative difference in delivery mode. Conclusion: The BSS-R is both conceptually and statistically comparable between different versions of the tool suggesting the utility of the measure for international comparative studies

Construct and content validity of the Greek version of the Birth Satisfaction Scale (G-BSS)

Background: ‘Birth Satisfaction’ is a term that encompasses a woman’s evaluation of her birth experience. The term includes factors such as her appraisal of the quality of care she received, a personal assessment of how she coped, and her reconstructions of what happened on that particular day. Her accounts may be accurate or skewed, yet correspond with her reality of how events unfolded. Objective: To evaluate properties of an instrument designed to measure birth satisfaction in a Greek population of postnatal women. Study design: We assessed factor structure, internal consistency, divergent validity and known-groups discriminant validity of the 30-item Greek Birth Satisfaction Scale – Long Form (30-item G-BSS-LF) and its revised version the 10-item Greek-BSS-Revised (10-item-G-BSS-R), using survey data collected in Athens. Participants: A convenience sample of healthy Greek postnatal women (n = 162) aged 22–46 years who had delivered between 34 and 42 weeks’ gestation. Results: The 30-item-G-BSS-LF performed poorly in terms of factor structure. The short-form 10-item-G-BSS-R performed well in terms of measurement replication of the English equivalent version as a multidimensional instrument. The short-form 10-item-G-BSS-R comprises three subscales which measure distinct but correlated domains of: (1) quality of care provision (4 items), (2) women’s personal attributes (2 items), and (3) stress experienced during labour (4 items). Key conclusions: The 10-item-G-BSS-R is a valid and reliable multidimensional psychometric instrument for measuring birth satisfaction in Greek postnatal women

The barriers to and enablers of providing reasonably adjusted health services to people with intellectual disabilities in acute hospitals: evidence from a mixed-methods study.

OBJECTIVE: To identify the factors that promote and compromise the implementation of reasonably adjusted healthcare services for patients with intellectual disabilities in acute National Health Service (NHS) hospitals. DESIGN: A mixed-methods study involving interviews, questionnaires and participant observation (July 2011-March 2013). SETTING: Six acute NHS hospital trusts in England. METHODS: Reasonable adjustments for people with intellectual disabilities were identified through the literature. Data were collected on implementation and staff understanding of these adjustments. RESULTS: Data collected included staff questionnaires (n=990), staff interviews (n=68), interviews with adults with intellectual disabilities (n=33), questionnaires (n=88) and interviews (n=37) with carers of patients with intellectual disabilities, and expert panel discussions (n=42). Hospital strategies that supported implementation of reasonable adjustments did not reliably translate into consistent provision of such adjustments. Good practice often depended on the knowledge, understanding and flexibility of individual staff and teams, leading to the delivery of reasonable adjustments being haphazard throughout the organisation. Major barriers included: lack of effective systems for identifying and flagging patients with intellectual disabilities, lack of staff understanding of the reasonable adjustments that may be needed, lack of clear lines of responsibility and accountability for implementing reasonable adjustments, and lack of allocation of additional funding and resources. Key enablers were the Intellectual Disability Liaison Nurse and the ward manager. CONCLUSIONS: The evidence suggests that ward culture, staff attitudes and staff knowledge are crucial in ensuring that hospital services are accessible to vulnerable patients. The authors suggest that flagging the need for specific reasonable adjustments, rather than the vulnerable condition itself, may address some of the barriers. Further research is recommended that describes and quantifies the most frequently needed reasonable adjustments within the hospital pathways of vulnerable patient groups, and the most effective organisational infrastructure required to guarantee their use, together with resource implications

Groundwater residence time in the Kulnura-Mangrove Mountain Plateau (Gosford, NSW, Australia)

The Kulnura-Mangrove Mountain plateau consists of the catchments of Mangrove, Narara, Mooney Mooney, and Ourimbah Creeks, and Wyong River. Groundwater plays a key role in sustaining stream flow within these catchments. Estimates indicate up to 50% of annual stream flow is derived from baseflow. The local community water supply relies on the groundwater within the elevated Hawkesbury- Narrabeen sandstone plateau. Furthermore, the Gosford-Wyong Councils’ Water Authority (WSA) is the third largest in NSW and utilises many of the streams flowing from the sandstone plateau for municipal water supply. It is anticipated that the WSA will provide municipal water for 319 000 persons by the year 2010. The increasing volumes of groundwater being extracted and changing land use have the potential to cause damage to the fresh water aquifer through contamination and aquifer depletion. A hydrogeochemical survey (2006-2009) has been conducted in NSW Dept of Water and Energy (DWE) monitoring wells across the plateau in order to determine groundwater residence times. Groundwater was analysed for major ions, minor and trace elements, H2O 18O and 2H, 13CDIC, 87Sr/86Sr, 14CDIC, and 3H, and complemented with mineralogical and isotopic information obtained from soil and drill chips collected during well construction. Water stable isotopes confirm the meteoric origin of the groundwater with most values plotting on the local meteoric water line. Localised evaporative trends suggest recharge with evaporated groundwater stored in ponds. Shallow groundwaters have 3H and 14C activities consistent with modern recharge (Fig 1). Carbon “bomb pulse” signatures of up to 116.8 pmC are found in the central areas of the plateau. The thin soils, lack of carbonates in the intensely weathered near-surface Hawkesbury sandstone, and the shallow depth of the water samples is consistent with the 3H results measured, suggesting minimal dilution of the original 14C. Input of this data into a southern hemisphere bomb pulse model [1] suggest potential recharge during the 1990´s, coinciding with sustained wet conditions and above average rainfalls experienced during this period. Fig. 1. 14C vs 3H plot of groundwater samples in the Kulnura- Mangrove Mountain Plateau Deeper groundwaters have lower 14C and 3H activities in some cases close to background level (Fig. 1). The quantifiable 3H suggests residence times of <70 a. However, non-corrected 14C residence times are submodern (>500 a). This apparent discrepancy can be explained by either mixing with older waters or dissolution of carbonates. The good correlation of total dissolved inorganic carbon (TDIC) and Ca (R2=0.8), 13CTDIC in groundwater and mineralogy results from drill chips suggest that dissolution of dispersed carbonates is taking place. The deepest groundwaters show the most difference in residence time across the study area. The eastern and western plateaus yield old groundwater with 14C corrected residence times of around 9 ka and 4 ka respectively. However, the groundwater at equivalent depths in the central plateau was found to be considerably younger with residence times of <70 a

Linking Repetition Priming, Recognition, and Source Memory: A Single-System Signal-Detection Account

We present new behavioral data and modeling that links priming, recognition, and source memory. In four experiments, we found that the magnitude of the priming effect, as measured with identification response time in a gradual clarification task, was (1) greater for studied items receiving correct source decisions than incorrect source decisions, and (2) increased as confidence in the source decision increased. Building on the framework for modeling recognition and priming proposed by Berry, Shanks, Speekenbrink, and Henson (2012), we developed a single-system model in which source memory decisions are driven by the same memory strength signal as recognition and priming. We formally compare the model against a multiple-systems model, in which the (implicit) memory signal driving priming is distinct to the (explicit) one driving recognition and source memory. The single-system model reproduces the qualitative patterns of the association between source memory and priming better than the multiple-systems model. Comparison of the quantitative fits was not as clear-cut, however: the single-system model tended to fit better in Experiments 1 and 2, but not in Experiments 3A and 3B, where the observed association between priming and recognition was weaker. Our investigation is an initial attempt at linking priming, recognition, and source memory in the same modelling framework, and provides a basis for further exploration and refinement

“I can’t skip it”: does free report improve accuracy in false memories?

Strategic monitoring of recognition memory by children and adults was examined using a semantic DRM procedure. Children (7- and 10-year-olds) and adults (overall N?=?393) studied lists of semantically related words either incidentally or intentionally and were tested with old items, new items and critical lures to judge as old or new. Participants either made a decision about every item they saw (forced report), or they had the opportunity to withhold answers they were uncertain about (free report). Children were less likely to withhold an answer than adults. However, 7-year-olds were more able to resist false memories when given the opportunity to withhold an answer compared to 10-year-olds or adults. In contrast, adults were unable to improve false memory accuracy. These data suggest that once semantically induced false memories have been encoded they are amenable to strategic monitoring at retrieval in children but not adults