Modern carbonate mound systems

Carbonate mounds are prominent features throughout the geological record. In many hydrocarbon provinces, they form prime reservoir structures. But recent investigations have increasingly reported occurrences of large mound clusters at the surface of the seabed, or buried at shallow depth on modern ocean margins, and in particular in basins rich in hydrocarbons. Such exciting new observations along the West-European margin are promising for elucidating the setting and environment of modern carbonate mounds, but at the same time they confront us with puzzling or sometimes contradictory observations in the quest for their genesis.Spectacular cold-water coral communities have colonized such mounds, but convincing arguments for recognizing them as prime builders are still lacking. The geological record provides ample evidence of microbial mediation in mound build-up and stabilisation, but as long as mound drilling is lacking, we have no opportunity to verify the role of such processes and identify the key actors in the earliest stage of onset and development of modern mounds. Some evidence from the past record and from present very-high resolution observations in the shallow seabed suggest an initial control by fluid venting, and fluid migration pathways have been imaged or are tentatively reconstructed by modelling in the concerned basins, but the ultimate link in the shallow subsurface seems still to elude a large part of our efforts. Surface sampling and analyses of both corals and surface sediments have largely failed in giving any conclusive evidence of present-day or recent venting in the considered basins. But on the other hand, applying rigourously the interpretational keys derived from e.g. Porcupine Seabight settings off NW Ireland on brand new prospective settings e.g. on the Moroccan margin have resulted in the discovery of totally new mound settings, in the middle of a field of giant, active mud volcanoes. Keys are apparently working, but we still do not understand how or why. We are no doubt facing complex systems at the interface between the Biosphere and the Geosphere, owing their genesis and spectacular growth to a complex woven of internal and external controls, feedback and process relay processes

The Pen Duick Escarpment off Morocco: A promising biogeochemically active carbonate mound laboratory (MiCROSYSTEMS)

Carbonate mud mounds, found in marine environments from shallow- to deep-water settings, span from Proterozoic to recent times. During the past decades, numerous active venting fields were discovered in deep marine environments and became a subject of extensive study for marine scientists. Mound building seems to be a fundamental but still enigmatic strategy for life. Various arguments suggest that microorganisms are playing a major role in the reef development, mound formation and biodiversity. Therefore, it is important to evaluate the microbial mediated processes of carbonate precipitation.Cold-water coral reefs thriving on carbonate mounds were discovered in the late 1990’s off western Ireland. An exploratory cruise of RV Belgica in 2002 off Morocco has led to the discovery of apparently juvenile mounds in water depths of 500-600 m, topping a cliff - the Pen Duick escarpment - flanked by giant mud volcanoes. Subsequent cruises have confirmed the colonization by deep-water corals and have unveiled extensive fields of seep-related carbonate crusts in the off-reef regions. Long cores taken in 2004 indicate that the ‘Pen Duick’ mounds, in which microbial action was demonstrated by a strong emission of hydrogen sulphide, may be considered as giant biogeochemical reactors. The mound sediments were dated 2 kyrs B.P. at the surface and 20 kyrs B.P. in a depth of 6 mbsf.A 450 cm long gravity core, coming from one of these juvenile mounds, was sampled and analyzed for mineralogy, stable isotopes composition, geochemistry, and microbial communities. Most of the sediment consists of calcite (coccoliths), quartz and dolomite. At a depth of 4 mbsf, we found hardened nodule-like structures, embedded in grey mud containing cold-water coral pieces. The presence of 20-30% of dolomite, in the carbonate phase, suggests a microbial influence during mineral formation. Preliminary results of the pore water geochemistry indicate a reactive sulphate – methane interface at 3.8 mbsf. In this layer we focused our studies on the microbial communities, such as methanogens, methanotrophs and sulphate reducers. The trend of the d13C values in digenetic carbonate supports the assumption of microbial activity in this section of the core. In order to define the primary microbial community involved in carbonate precipitation, we did direct culturing, DNA isolation and PCR analysis of three functional genes, the a subunit (mcrA) of the methyl-coenzyme M reductase (MCR), the a subunit (pmoA) of the particulate methane monooxygenase (MMO) and the a and ß subunits (dsrA and dsrB, respectively) of the dissimilatory sulfite reductase (DSR). These enzymes are involved in methanogenesis, methanotrophy and sulphate reduction biochemical pathways, respectively. In summary, our initial results demonstrate that the Pen Duick carbonate mound can be considered as a natural laboratory in which to study cold-water coral ecosystems associated with microbial activity

Vif is a RNA chaperone that could temporally regulate RNA dimerization and the early steps of HIV-1 reverse transcription

HIV-1 Vif (viral infectivity factor) is associated with the assembly complexes and packaged at low level into the viral particles, and is essential for viral replication in non-permissive cells. Viral particles produced in the absence of Vif exhibit structural defects and are defective in the early steps of reverse transcription. Here, we show that Vif is able to anneal primer tRNALys3 to the viral RNA, to decrease pausing of reverse transcriptase during (–) strand strong-stop DNA synthesis, and to promote the first strand transfer. Vif also stimulates formation of loose HIV-1 genomic RNA dimers. These results indicate that Vif is a bona fide RNA chaperone. We next studied the effects of Vif in the presence of HIV-1 NCp, which is a well-established RNA chaperone. Vif inhibits NCp-mediated formation of tight RNA dimers and hybridization of tRNALys3, while it has little effects on NCp-mediated strand transfer and it collaborates with nucleocapsid (NC) to increase RT processivity. Thus, Vif might negatively regulate NC-assisted maturation of the RNA dimer and early steps of reverse transcription in the assembly complexes, but these inhibitory effects would be relieved after viral budding, thanks to the limited packaging of Vif in the virions

Boolean delay equations on networks: An application to economic damage propagation

We introduce economic models based on Boolean Delay Equations: this formalism makes easier to take into account the complexity of the interactions between firms and is particularly appropriate for studying the propagation of an initial damage due to a catastrophe. Here we concentrate on simple cases, which allow to understand the effects of multiple concurrent production paths as well as the presence of stochasticity in the path time lengths or in the network structure. In absence of flexibility, the shortening of production of a single firm in an isolated network with multiple connections usually ends up by attaining a finite fraction of the firms or the whole economy, whereas the interactions with the outside allow a partial recovering of the activity, giving rise to periodic solutions with waves of damage which propagate across the structure. The damage propagation speed is strongly dependent upon the topology. The existence of multiple concurrent production paths does not necessarily imply a slowing down of the propagation, which can be as fast as the shortest path.Comment: Latex, 52 pages with 22 eps figure

Pharmacological conditioning for juvenile idiopathic arthritis:a potential solution to reduce methotrexate intolerance

Background: Methotrexate (MTX) therapy has proven to be a successful and safe treatment for Juvenile Idiopathic Arthritis (JIA). Despite the high efficacy rates of MTX, treatment outcomes are often complicated by burdensome gastro-intestinal side effects. Intolerance rates for MTX in children are high (approximately 50%) and thus far no conclusive effective treatment strategies to control for side effects have been found. To address this need, this article proposes an innovative research approach based on pharmacological conditioning, to reduce MTX intolerance. Presentation of the hypothesis: A collaboration between medical psychologists, pediatric rheumatologists, pharmacologists and patient groups was set up to develop an innovative research design that may be implemented to study potential improved control of side effects in JIA, by making use of the psychobiological principles of pharmacological conditioning. In pharmacological conditioning designs, learned positive associations from drug therapies (conditioning effects) are integrated in regular treatment regimens to maximize treatment outcomes. Medication regimens with immunosuppressant drugs that made use of pharmacological conditioning principles have been shown to lead to optimized therapeutic effects with reduced drug dosing, which might ultimately cause a reduction in side effects. Testing the hypothesis: This research design is tailored to serve the needs of the JIA patient group. We developed a research design in collaboration with an interdisciplinary research group consisting of patient representatives, pediatric rheumatologists, pharmacologists, and medical psychologists. Implications of the hypothesis: Based on previous experimental and clinical findings of pharmacological conditioning with immune responses, we propose that the JIA patient group is particularly suited to benefit from a pharmacological conditioning design. Moreover, findings from this study may potentially also be promising for other patient groups that endure long-lasting drug therapies

Impact of Dose Adaptations Following Voriconazole Therapeutic Drug Monitoring in Pediatric Patients

This manuscript was derived from chapter 9 of the PhD thesis by Vincent Lempers done at Radboudumc, which can be found at: https://repository.ubn.ru.nl/bitstream/handle/2066/157075/157075.pdf?sequence=1 AW is supported by the Wellcome Trust Strategic Award (grant 097377), and the MRC Centre for Medical Mycology (grant MR/N006364/1) at the University of Aberdeen. Funding: this study was funded by the Department of Pharmacy RadboudumcPeer reviewedPostprin

Limit on suppression of ionization in metastable neon traps due to long-range anisotropy

This paper investigates the possibility of suppressing the ionization rate in a magnetostatic trap of metastable neon atoms by spin-polarizing the atoms. Suppression of the ionization is critical for the possibility of reaching Bose-Einstein condensation with such atoms. We estimate the relevant long-range interactions for the system, consisting of electric quadrupole-quadrupole and dipole-induced dipole terms, and develop short-range potentials based on the Na_2 singlet and triplet potentials. The auto-ionization widths of the system are also calculated. With these ingredients we calculate the ionization rate for spin-polarized and for spin-isotropic samples, caused by anisotropy of the long-range interactions. We find that spin-polarization may allow for four orders of magnitude suppression of the ionization rate for Ne. The results depend sensitively on a precise knowledge of the interaction potentials, however, pointing out the need for experimental input. The same model gives a suppression ratio close to unity for metastable xenon in accordance with experimental results, due to a much increased anisotropy in this case.Comment: 15 pages including figures, LaTex/RevTex, uses epsfig.st

HIV Types, Groups, Subtypes and Recombinant Forms: Errors in Replication, Selection Pressure and Quasispecies

HIV-1 is a chimpanzee virus which was transmitted to humans by several zoonotic events resulting in infection with HIV-1 groups M P, and in parallel transmission events from sooty mangabey monkey viruses leading to infections with HIV-2 groups A H. Both viruses have circulated in the human population for about 80 years. In the infected patient, HIV mutates, and by elimination of some of the viruses by the action of the immune system individual quasispecies are formed. Along with the selection of the fittest viruses, mutation and recombination after superinfection with HIV from different groups or subtypes have resulted in the diversity of their patterns of geographic distribution. Despite the high variability observed, some essential parts of the HIV genome are highly conserved. Viral diversity is further facilitated in some parts of the HIV genome by drug selection pressure and may also be enhanced by different genetic factors, including HLA in patients from different regions of the world. Viral and human genetic factors influence pathogenesis. Viral genetic factors are proteins such as Tat, Vif and Rev. Human genetic factors associated with a better clinical outcome are proteins such as APOBEC, langerin, tetherin and chemokine receptor 5 (CCR5) and HLA B27, B57, DRB1{*}1303, KIR and PARD3B. Copyright (C) 2012 S. Karger AG, Base

Preliminary statement of the onshore and offshore meso-cenozoic tectonic data in western Belgium and northern France

A first comparison between the onshore and offshore informations leads to a tectonic sketch of the South North-Sea and the Detroit du Pas-de-Calais (Strait of Dover): The more striking feature is that significant structures (North Hinder and Gravelines structures) seem to extend offshore the transverse faults known in the Paleozoic beds of Pas-de-Calais and Boulonnais. This set of deformations can be interpreted as reactions of the thick Meso-Cenozoic cover to fractures acting in the Paleozoic basement. Towards the South, between the Zone de Cisaillement Nord-Artois and the Faille de Montreuil-Bassurelle, along the Weald Artois Axis, where the Meso-Cenozoic cover is thinner, the transverse faults, probably dependent upon the same fractures, have a dextral strike-slip character both in Paleozoic basement and in the Cretaceous strata. The size and significance of this assumed deep faults are discussed in the framework of a model of this part of the Southern North-Sea

Deep Submarine Giant Scours in northern Gulf of Cadiz (offshore SW Iberia): a singular case of sedimentary and tectonic coupling?

Multi-beam swath bathymetry carried out in NW Gulf of Cadiz(offshore SW Iberia - MATESPRO campaign) revealed several intriguing morphologic features, lying at depths between -3900 and - 4700 m., in an area characterized by very shallow general slope gradients (dipping approximately 0.4 degrees). These three dimensional features are characterized by elliptical crescent shapes of kilometric length (major axis around 5 km), displaying internal escarpments up to 100 m high and slopes varying between 6 and 14 degrees.A single channel seismic profile acquired across two of these features shows that they have a sub-surface composite structure. The internal part of the crescent consists of a depression filled up with upslope prograding sedimentary units developing towards the scarp that sharply truncates the sedimentary horizons. The growth processes of these structures appears to be by retrogressive displacement of a morphological scarp and remobilization followed by deposition of the eroded material in front of the scarp, prograding towards it. This process is similar to the development of contourite bodies in which the current direction is parallel to the scarp, whilst in the present case the flow direction may be mainly perpendicular to the scarp.These units are overlain by a sub-horizontal turbidite-like sedimentary unit that partially infills the depression. A deep multi-channel seismic profile across the same structure revealed the existence of an underlying thrust, part of a major structure, the Gulf of Cadiz Accretionary Prism. These singular features are interpreted as the coupled of sedimentary and tectonic processes. It is proposed that the Deep Submarine Giant Scours formed when a local morphologic irregularity, corresponding to the bathymetric expression of movement on an underlying thrust, is enhanced by the erosional activity of turbidity and thermohaline bottom currents driven by the formation of local eddies in front of the scarp. The drift results from the re-deposition of the eroded material in the same location