Intrinsic limits to gene regulation by global crosstalk

Gene regulation relies on the specificity of transcription factor (TF) - DNA interactions. In equilibrium, limited specificity may lead to crosstalk: a regulatory state in which a gene is either incorrectly activated due to noncognate TF-DNA interactions or remains erroneously inactive. We present a tractable biophysical model of global crosstalk, where many genes are simultaneously regulated by many TFs. We show that in the simplest regulatory scenario, a lower bound on crosstalk severity can be analytically derived solely from the number of (co)regulated genes and a suitable parameter that describes binding site similarity. Estimates show that crosstalk could present a significant challenge for organisms with low-specificity TFs, such as metazoans, unless they use appropriate regulation schemes. Strong cooperativity substantially decreases crosstalk, while joint regulation by activators and repressors, surprisingly, does not; moreover, certain microscopic details about promoter architecture emerge as globally important determinants of crosstalk strength. Our results suggest that crosstalk imposes a new type of global constraint on the functioning and evolution of regulatory networks, which is qualitatively distinct from the known constraints acting at the level of individual gene regulatory elements

Strain monitoring of tapestries: results of a three-year research project

The outcomes of an interdisciplinary research project between conservators and engineers investigating the strain experienced by different areas of a tapestry are described. Two techniques were used: full-field monitoring using digital image correlation (DIC) and point measurements using optical fibre sensors. Results showed that it is possible to quantify the global strain across a discrete area of a tapestry using DIC; optical fibre and other sensors were used to validate the DIC. Strain maps created by the DIC depict areas of high and low strain and can be overlaid on images of the tapestry, creating a useful visual tool for conservators, custodians and the general public. DIC identifies areas of high strain not obvious to the naked eye. The equipment can be used in situ in a historic house. In addition the work demonstrated the close relationship between relative humidity and strain

Surface plasmon modes and the Casimir energy

We show the influence of surface plasmons on the Casimir effect between two plane parallel metallic mirrors at arbitrary distances. Using the plasma model to describe the optical response of the metal, we express the Casimir energy as a sum of contributions associated with evanescent surface plasmon modes and propagative cavity modes. In contrast to naive expectations, the plasmonic modes contribution is essential at all distances in order to ensure the correct result for the Casimir energy. One of the two plasmonic modes gives rise to a repulsive contribution, balancing out the attractive contributions from propagating cavity modes, while both contributions taken separately are much larger than the actual value of the Casimir energy. This also suggests possibilities to tailor the sign of the Casimir force via surface plasmons.Comment: 4 pages, 3 figures, revtex

Semiclassical Gravity Theory and Quantum Fluctuations

We discuss the limits of validity of the semiclassical theory of gravity in which a classical metric is coupled to the expectation value of the stress tensor. It is argued that this theory is a good approximation only when the fluctuations in the stress tensor are small. We calculate a dimensionless measure of these fluctuations for a scalar field on a flat background in particular cases, including squeezed states and the Casimir vacuum state. It is found that the fluctuations are small for states which are close to a coherent state, which describes classical behavior, but tend to be large otherwise. We find in all cases studied that the energy density fluctuations are large whenever the local energy density is negative. This is taken to mean that the gravitational field of a system with negative energy density, such as the Casimir vacuum, is not described by a fixed classical metric but is undergoing large metric fluctuations. We propose an operational scheme by which one can describe a fluctuating gravitational field in terms of the statistical behavior of test particles. For this purpose we obtain an equation of the form of the Langevin equation used to describe Brownian motion.Comment: In REVTEX. 20pp + 4 figures(not included, available upon request) TUTP-93-

Ultra-high-sensitivity two-dimensional bend sensor

A multicore fibre Fabry-Perot-based strain sensor interrogated with tandem interferometry for bend measurement is described. Curvature in two dimensions is obtained by measuring the difference in strain between three co-located low finesse Fabry-Perot interferometers formed in each core of the fibre by pairs of Bragg gratings. This sensor provides a responsivity enhancement of up to 30 times that of a previously reported fibre Bragg grating based sensor. Strain resolutions of 0.6 n epsilon/Hz(1/2) above 1 Hz are demonstrated, which corresponds to a curvature resolution of similar to 0.012 km(-1)/Hz(1/2)

Stochastic Spacetime and Brownian Motion of Test Particles

The operational meaning of spacetime fluctuations is discussed. Classical spacetime geometry can be viewed as encoding the relations between the motions of test particles in the geometry. By analogy, quantum fluctuations of spacetime geometry can be interpreted in terms of the fluctuations of these motions. Thus one can give meaning to spacetime fluctuations in terms of observables which describe the Brownian motion of test particles. We will first discuss some electromagnetic analogies, where quantum fluctuations of the electromagnetic field induce Brownian motion of test particles. We next discuss several explicit examples of Brownian motion caused by a fluctuating gravitational field. These examples include lightcone fluctuations, variations in the flight times of photons through the fluctuating geometry, and fluctuations in the expansion parameter given by a Langevin version of the Raychaudhuri equation. The fluctuations in this parameter lead to variations in the luminosity of sources. Other phenomena which can be linked to spacetime fluctuations are spectral line broadening and angular blurring of distant sources.Comment: 15 pages, 3 figures. Talk given at the 9th Peyresq workshop, June 200

Groups of two galaxies in SDSS: implications of colours on star formation quenching time-scales

We have devised a method to select galaxies that are isolated in their dark matter halo (N=1 systems) and galaxies that reside in a group of exactly two (N=2 systems). Our N=2 systems are widely-separated (up to $\sim$\,200\,$h^{-1}$\,kpc), where close galaxy-galaxy interactions are not dominant. We apply our selection criteria to two volume-limited samples of galaxies from SDSS DR6 with $M_{r}-5 \log_{10} h \leq$ -19 and -20 to study the effects of the environment of very sparse groups on galaxy colour. For satellite galaxies in a group of two, we find a red excess attributed to star formation quenching of 0.15\,$\pm$\,0.01 and 0.14\,$\pm$\,0.01 for the -19 and -20 samples, respectively, relative to isolated galaxies of the same stellar mass. Assuming N=1 systems are the progenitors of N=2 systems, an immediate-rapid star formation quenching scenario is inconsistent with these observations. A delayed-then-rapid star formation quenching scenario with a delay time of 3.3 and 3.7\,Gyr for the -19 and -20 samples, respectively, yields a red excess prediction in agreement with the observations. The observations also reveal that central galaxies in a group of two have a slight blue excess of 0.06\,$\pm$\,0.02 and 0.02\,$\pm$\,0.01 for the -19 and -20 samples, respectively, relative to N=1 populations of the same stellar mass. Our results demonstrate that even the environment of very sparse groups of luminous galaxies influence galaxy evolution and in-depth studies of these simple systems are an essential step towards understanding galaxy evolution in general.Comment: 17 pages, 11 figures, accepted to MNRA