46 research outputs found
A novel inhibitor of p75-neurotrophin receptor improves functional outcomes in two models of traumatic brain injury.
The p75 neurotrophin receptor is important in multiple physiological actions including neuronal survival and neurite outgrowth during development, and after central nervous system injury. We have discovered a novel piperazine-derived compound, EVT901, which interferes with p75 neurotrophin receptor oligomerization through direct interaction with the first cysteine-rich domain of the extracellular region. Using ligand binding assays with cysteine-rich domains-fused p75 neurotrophin receptor, we confirmed that EVT901 interferes with oligomerization of full-length p75 neurotrophin receptor in a dose-dependent manner. Here we report that EVT901 reduces binding of pro-nerve growth factor to p75 neurotrophin receptor, blocks pro-nerve growth factor induced apoptosis in cells expressing p75 neurotrophin receptor, and enhances neurite outgrowth in vitro Furthermore, we demonstrate that EVT901 abrogates p75 neurotrophin receptor signalling by other ligands, such as prion peptide and amyloid-ÎČ. To test the efficacy of EVT901 in vivo, we evaluated the outcome in two models of traumatic brain injury. We generated controlled cortical impacts in adult rats. Using unbiased stereological analysis, we found that EVT901 delivered intravenously daily for 1 week after injury, reduced lesion size, protected cortical neurons and oligodendrocytes, and had a positive effect on neurological function. After lateral fluid percussion injury in adult rats, oral treatment with EVT901 reduced neuronal death in the hippocampus and thalamus, reduced long-term cognitive deficits, and reduced the occurrence of post-traumatic seizure activity. Together, these studies provide a new reagent for altering p75 neurotrophin receptor actions after injury and suggest that EVT901 may be useful in treatment of central nervous system trauma and other neurological disorders where p75 neurotrophin receptor signalling is affected
Evaluation of the CloudSat surface snowfall product over Antarctica using ground-based precipitation radars
In situ observations of snowfall over the Antarctic Ice Sheet are scarce.
Currently, continent-wide assessments of snowfall are limited to information
from the Cloud Profiling Radar on board the CloudSat satellite, which has not been evaluated up to now. In this study,
snowfall derived from CloudSat is evaluated using three ground-based
vertically profiling 24 GHz precipitation radars (Micro Rain Radars: MRRs).
Firstly, using the MRR long-term measurement records, an assessment of the
uncertainty caused by the low temporal sampling rate of CloudSat (one revisit
per 2.1Â to 4.5Â days) is performed. The 10â90th-percentile temporal sampling
uncertainty in the snowfall climatology varies between 30 % and 40 %
depending on the latitudinal location and revisit time of CloudSat. Secondly,
an evaluation of the snowfall climatology indicates that the CloudSat
product, derived at a resolution of 1â latitude by 2â
longitude, is able to accurately represent the snowfall climatology at the
three MRR sites (biases < 15 %), outperforming ERA-Interim. For coarser
and finer resolutions, the performance drops as a result of higher omission
errors by CloudSat. Moreover, the CloudSat product does not perform well in
simulating individual snowfall events. Since the difference between the MRRs
and the CloudSat climatology are limited and the temporal uncertainty is
lower than current Climate Model Intercomparison Project Phase 5 (CMIP5)
snowfall variability, our results imply that the CloudSat product is valuable
for climate model evaluation purposes.</p
Performance of Pyridylthiourea-Polyethylenimine Polyplex for siRNA-Mediated Liver Cancer Therapy in Cell Monolayer, Spheroid, and Tumor Xenograft Models
Medical application of siRNAs relies on methods for delivering nucleic acids into the cytosol. Synthetic carriers, which assemble with nucleic acids into delivery systems, show promises for cancer therapy but efficiency remains to be improved. In here, the effectiveness of pyridylthioureaâpolyethylenimine (ÏPEI), a siRNA carrier that favors both polyplex disassembly and endosome rupture upon sensing the acidic endosomal environment, in 3 experimental models of hepatocellular cancer is tested. The ÏPEIâassisted delivery of a siRNA targeting the poloâlike kinase 1 into Huhâ7 monolayer produces a 90% cell death via a demonstrated RNA interference mechanism. Incubation of polyplex with Huhâ7 spheroids leads to siRNA delivery into the superficial first cell layer and a 60% reduction in spheroid growth compared to untreated controls. Administration of polyplexes into mice bearing subcutaneous implanted Huhâ7Luc tumors results in a reduced tumor progression, similar to the one observed in the spheroid model. Altogether, these results support the in vivo use of synthetic and dedicated polymers for increasing siRNAâmediated gene knockdown, and their clinical promise in cancer therapeutics
A novel antagonist of p75NTR reduces peripheral expansion and CNS trafficking of pro-inflammatory monocytes and spares function after traumatic brain injury
Avulsion dâune dent surnumĂ©raire incluse par dĂ©coupe puis repositionnement de lâĂ©pine nasale antĂ©rieure
Les avulsions de dents incluses sont dâune pratique courante. Le plus frĂ©quemment, le dĂ©gagement osseux nâa pas de consĂ©quences. Dans certains cas, il peut poser problĂšme, sâil concerne un futur site dâimplantation ou des structures anatomiques spĂ©cifiques, notamment dans le prĂ©maxillaire (Sukegawa et al. Case Rep Dent. 2015; 2015: 974169). Pour ces situations, la piĂ©zochirugie apparait comme une technique peu invasive puisque la dĂ©coupe est plus fine et plus prĂ©cise quâavec les instruments rotatifs (PavlıékovaĂ© et al. Int J Oral Maxillofac Surg. 2011 40:451-7). De plus, le fait de dĂ©couper un volet puis de le repositionner en fin dâintervention constitue un moyen de prĂ©server le volume osseux. Le cas rapportĂ© est celui dâune avulsion dâun mesiodens par dĂ©coupe puis repositionnement de lâĂ©pine nasale antĂ©rieure. Une jeune fille de 13 ans a Ă©tĂ© adressĂ©e par son orthodontiste pour avulsion dâun mesiodens avant prise en charge ODF. Un examen cone beam a prĂ©cisĂ© la position de cet odontoĂŻde. Dâune morphologie Ă©voquant une incisive conoĂŻde, la dent surnumĂ©raire se prĂ©sentait en position verticale ; sa couronne faisait saillie dans les fosses nasales, sous le vomer, et sa racine Ă©tait en arriĂšre de celles des incisives centrales. Lors dâune intervention menĂ©e sous anesthĂ©sie gĂ©nĂ©rale, lâENA a Ă©tĂ© dĂ©coupĂ©e par piĂ©zochirugie (incision en V de part et dâautre du plan mĂ©dian) et luxĂ©e vers le haut, sans dĂ©collement de la muqueuse nasale. LâodontoĂŻde a Ă©tĂ© sectionnĂ© au collet. Sa couronne dâabord puis sa racine ont Ă©tĂ© avulsĂ©es. Enfin, lâENA a Ă©tĂ© repositionnĂ©e et fixĂ©e par une vis dâostosynthĂšse positionnĂ©e lĂ©gĂšrement en biais. Les suites ont Ă©tĂ© bonnes. La vis dâostĂ©osynthĂšse a Ă©tĂ© dĂ©posĂ©e un an plus tard. Lâavulsion de cette dent, profondĂ©ment incluse, par les techniques conventionnelles utilisant des instruments rotatifs aurait entraĂźnĂ© un dĂ©labrement important. Compte tenu de la situation inversĂ©e de lâaxe de la dent, il aurait Ă©tĂ© difficile de la retirer par un abord palatin. De plus, son axe vertical compliquait la sĂ©paration couronne-racine, qui nâaurait Ă©tĂ© possible que par un accĂšs nasal. Le choix dâune technique conservatrice de lâos (dĂ©coupe puis repositionnement) semblait judicieux dans la mesure oĂč il autorisait une bonne exposition du site et le morcellement cervical de la dent, tout en Ă©pargnant le volume osseux. Le fait de ne pas avoir dĂ©collĂ© la muqueuse palatine a probablement contribuĂ© Ă la trophicitĂ© et la cicatrisation de lâENA repositionnĂ©e. Les opĂ©rateurs ont pris le soin de ne pas placer la vis dâostĂ©osynthĂšse dans la suture entre les deux parties droite et gauche de lâENA, afin de ne pas risquer de sĂ©parer ces deux os. Ce cas illustre une technique minimalement invasive qui a permis lâavulsion dâune dent surnumĂ©raire profondĂ©ment incluse en position verticale inversĂ©e, sans entraĂźner de lĂ©sion des dents voisines ni de la muqueuse nasale, tout en prĂ©servant le volume osseux de lâENA
Les entreprises nummriques sont-elles responsables? (Are Digital Companies Responsible?)
Antibody affinity disturbance in the immunoglobulin immune response to mucosal antigenic stimulation in lung cancer. The betalactoglobulin model
Severe systemic cytomegalovirus infections in patients with steroid-refractory ulcerative colitis treated by an oral microemulsion form of cyclosporine: report of two cases.
International audienceCMV reactivation is frequently observed in acute flares of ulcerative colitis (UC), particularly those which do not respond to intravenous steroids. Several recent series have suggested that, in most cases, CMV reactivation does not lead to severe complications and resolves spontaneously with the UC flare and discontinuation of immunosuppression. In the present paper, we describe two patients with active UC who developed a severe systemic CMV infection during a treatment with an oral microemulsion form of cyclosporine. This is of concern, particularly in a context of increasing use of immunosuppressive drugs in UC. We propose a prophylactic and curative approach to decrease morbidity related to CMV infection in active UC