XML in Motion from Genome to Drug

Information technology (IT) has emerged as a central to the solution of contemporary genomics and drug discovery problems. Researchers involved in genomics, proteomics, transcriptional profiling, high throughput structure determination, and in other sub-disciplines of bioinformatics have direct impact on this IT revolution. As the full genome sequences of many species, data from structural genomics, micro-arrays, and proteomics became available, integration of these data to a common platform require sophisticated bioinformatics tools. Organizing these data into knowledgeable databases and developing appropriate software tools for analyzing the same are going to be major challenges. XML (eXtensible Markup Language) forms the backbone of biological data representation and exchange over the internet, enabling researchers to aggregate data from various heterogeneous data resources. The present article covers a comprehensive idea of the integration of XML on particular type of biological databases mainly dealing with sequence-structure-function relationship and its application towards drug discovery. This e-medical science approach should be applied to other scientific domains and the latest trend in semantic web applications is also highlighted

Sensor Network Infrastructure for AMI in Smart Grid

AbstractUtility companies incur financial loss due to transmission losses as well as non technical losses like power theft and billing irregularities. Smart meters with the required functionalities along with an intelligent grid form an Automated Meter Reading (AMR) infrastructure which can improve the distribution efficiency. AMR infrastructure can be expanded by allowing for bi-directional communication between the grid components to form an Advanced Metering Infrastructure (AMI). This helps for the smooth interaction between grid components to load and off-load the distributed energy sources while supporting an efficient Demand Side Management of power. Communication technologies play a vital role in the implementation of smart grid and Wireless Sensor Networks are gaining attraction in this field. Routing protocols with high reliability, low latency and that can co-exist with the established protocols are the need of the hour. Routing Protocol for Low Power Lossy Networks (RPL) fit into this space well. An attempt to build an automated infrastructure using RPL for collecting the metering information, which at a later stage can be easily upgraded to an AMI setup by simply adding the supporting end devices for a much more intelligent smart grid, is carried out in this pape

In vitro studies on effects of plant growth regulators on callus and suspension culture biomass yield from Gymnema sylvestre R.Br

Callus cultures were initiated from nodal segments and leaf explants of Gymnema sylvestre on Murashige and Skoog (1962) medium containing basic salts and 30 g/l sucrose supplemented with different concentrations (0.10, 0.25, 0.5, 1.0, 2.0 and 5.0 mg/l) of 2,4-dichlorophenoxy acetic acid (2,4-D), -naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), kinetin (KN) and 6-benzyladenine (BA). Callus induction was observed in 0.5 mg/l of 2, 4-D supplemented medium for both explants. At the initial stage, some parts of explants enlarged and gave raise to pale yellowish calli after 2-3 weeks of incubation. The harvested cell biomass was subjected to extraction of active principles. In this study, cell biomass extracts were compared with extracts from leaves of naturally growing gymnema plants. HPLC analysis of these extracts showed that the main components of the active principles namely gymnemic acids and gymnemagenin were present in sufficiently large amounts in the cultured undifferentiated cell

In vitro production of growth regulators and phosphatase activity by phosphate solubilizing bacteria

Crops absorb phosphorous in the form of soluble orthophosphate ion. The solubility of phosphate is inhibited by the presence of iron and aluminium in acidic soils and calcium in neutral and alkaline soils. This leads to fixation of phosphorous, making it not available to crop plants. The phosphate solubilizing bacteria (phosphobacteria) secretes some kinds of organic acids which act on insoluble phosphates and convert the same into soluble form, thus providing phosphorous to plant. An experiment was conducted to enumerate the population density of phosphobacteria in the rhizosphere soils of brinjal, chilly, cotton, green grain, groundnut, maize, paddy, ragi, sorghum and turmeric using Ketznelson and Bose medium following dilution plate technique. Efforts have been made to isolate phosphobacteria from these soils and isolated strains were inoculated in specific media containing specific substrates to produce growth regulating substances such as IAA and GA3 and phosphatase enzyme. The result showed that the population levels of phosphobacteria were higher in the rhizosphere soil of groundnut plant. Further, all the strains of phosphobacteria were able to produce phytohormones and phosphatase enzyme under in vitro conditions.Keywords: In vitro, phosphobacteria, growth regulator

Computational estimation of scene structure through texture gradient cues

Analyzing the depth structure implied in two-dimensional images is one of the most active research areas in computer vision. Here, we propose a method of utilizing texture within an image to derive its depth structure. Though most approaches for deriving depth from a single still image utilize luminance edges and shading to estimate scene structure, relatively little work has been done to utilize the abundant texture information in images. Our new approach begins by analyzing the two cues of local spatial frequency and orientation distributions of the textures within an image, which are used to compute the local slant information across the image. The slant and frequency information are merged to create a unified depth map, providing an important channel for image structure information that can be combined with other available cues. The capabilities of the algorithm are illustrated for a variety of images of planar and curved surfaces under perspective projection, in most of which the depth structure is effortlessly perceived by human observers. Since these operations are readily implementable in neural hardware in early visual cortex, they therefore represent a model of the human perception of the depth structure of images from texture gradient cues

In vitro multiplication of Ocimum gratissimum L. through direct regeneration

The objective of this study was to develop a rapid system for regeneration of the important medicinal plant, Ocimum gratissimum L, from nodal explant. Single node explants were inoculated on basal MS (Murashige and Skoog, 1962) medium containing 3% (w/v) sucrose, supplemented with differentconcentrations and combinations of 6-benzylaminopurine (BAP), kinetin (KN), indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) for direct plant regeneration. Maximum numbers of shoot (14.3±1.5) were observed on the medium containing 0.5 mg/l BAP and 0.25 mg/l IAA after four weeks of culture. Regenerated shoots were separated and rooted on same half strength MS medium supplemented with 0.5 mg/l of IAA alone for three weeks. Well-developed complete plantlets were transferred on to speciallymade plastic cup containing soilrite. Acclimatized plantlets were successfully grown in garden soi

Efficacy of certain fungicides against Phomopsis theae under in vitro conditions

Four contact fungicides, Blitox (copper oxychloride), Kocide (copper hydroxide), Mancozeb (dithane M-45) and Bordeaux mixture and four systemic fungicides, Baycor (bitertanol), Calixin (tridemorph), Contaf (hexaconazole) and Bavistin (carbendazim) were evaluated against Phomopsis theae under in vitro condition. The results indicated that carbendazim was found to be the most effective in suppressing the growth followed by dithane M-45. Among the different concentrations tested, carbendazim at 4 ppm and dithane M-45 at 8 ppm were found to be optimum for the control of pathogen’s growth.Keywords: Phomopsis theae, fungicides, in vitro screening, phomopsis canke

Permanent His-bundle Pacing in Pediatrics and Congenital Heart Disease

Permanent His-bundle pacing has been gaining popularity in the adult population requiring cardiac resynchronization therapy. Initial procedural challenges are being overcome, and this method of pacing has been shown to improve left ventricular function and heart failure symptoms secondary to ventricular dyssynchrony. Though the etiologies of ventricular dyssynchrony may differ in children and those with congenital heart disease than in adults with structurally normal hearts, His-bundle pacing may also be a preferred option in these groups to restore more physiologic electric conduction and improve ventricular function. We present a review of the current literature and suggested directions involving deploying permanent His-bundle pacing in the pediatric and congenital heart disease population

Endothelial Dysfunction and Diabetes: Effects on Angiogenesis, Vascular Remodeling, and Wound Healing

Diabetes mellitus (DM) is a chronic metabolic disorder characterized by inappropriate hyperglycemia due to lack of or resistance to insulin. Patients with DM are frequently afflicted with ischemic vascular disease or wound healing defect. It is well known that type 2 DM causes amplification of the atherosclerotic process, endothelial cell dysfunction, glycosylation of extracellular matrix proteins, and vascular denervation. These complications ultimately lead to impairment of neovascularization and diabetic wound healing. Therapeutic angiogenesis remains an attractive treatment modality for chronic ischemic disorders including PAD and/or diabetic wound healing. Many experimental studies have identified better approaches for diabetic cardiovascular complications, however, successful clinical translation has been limited possibly due to the narrow therapeutic targets of these agents or the lack of rigorous evaluation of pathology and therapeutic mechanisms in experimental models of disease. This paper discusses the current body of evidence identifying endothelial dysfunction and impaired angiogenesis during diabetes