How to Measure Molecular Forces in Cells: A Guide to Evaluating Genetically-Encoded FRET-Based Tension Sensors

The ability of cells to sense and respond to mechanical forces is central to a wide range of biological processes and plays an important role in numerous pathol- ogies. The molecular mechanisms underlying cellular mech- anotransduction, however, have remained largely elusive because suitable methods to investigate subcellular force propagation were missing. Here, we review recent advances in the development of biosensors that allow molecular force measurements. We describe the underlying principle of currently available techniques and propose a strategy to systematically evaluate new Fo ̈ rster resonance energy trans- fer (FRET)-based biosensor

Predicting Beef Palatability

The search for factors influencing the palatability of beef continues. The relative importance of variables in the USDA quality grading system that currently predicts acceptability and palatability has been questioned. The effect of marbling on palatability is one of the more controversial aspects. Marbling is one of the prime factors in determining the quality grade of a beef carcass and researchers have shown that other variables may play an equal or more important role influencing the eating quality of beef. The purpose of this study was to evaluate the accuracy of commonly-used predictors of palatability and to consider the possible influence of other carcass measures as predictors of palatability. Palatability, as defined in this study, was measured by the subjective taste panel scores for flavor, juiciness and tenderness. Tenderness mas measured objectively with the Warner-Bratzler shear instrument

Estimating the incidence, prevalence and true cost of asthma in the UK: secondary analysis of national stand-alone and linked databases in England, Northern Ireland, Scotland and Wales-a study protocol.

INTRODUCTION: Asthma is now one of the most common long-term conditions in the UK. It is therefore important to develop a comprehensive appreciation of the healthcare and societal costs in order to inform decisions on care provision and planning. We plan to build on our earlier estimates of national prevalence and costs from asthma by filling the data gaps previously identified in relation to healthcare and broadening the field of enquiry to include societal costs. This work will provide the first UK-wide estimates of the costs of asthma. In the context of asthma for the UK and its member countries (ie, England, Northern Ireland, Scotland and Wales), we seek to: (1) produce a detailed overview of estimates of incidence, prevalence and healthcare utilisation; (2) estimate health and societal costs; (3) identify any remaining information gaps and explore the feasibility of filling these and (4) provide insights into future research that has the potential to inform changes in policy leading to the provision of more cost-effective care. METHODS AND ANALYSIS: Secondary analyses of data from national health surveys, primary care, prescribing, emergency care, hospital, mortality and administrative data sources will be undertaken to estimate prevalence, healthcare utilisation and outcomes from asthma. Data linkages and economic modelling will be undertaken in an attempt to populate data gaps and estimate costs. Separate prevalence and cost estimates will be calculated for each of the UK-member countries and these will then be aggregated to generate UK-wide estimates. ETHICS AND DISSEMINATION: Approvals have been obtained from the NHS Scotland Information Services Division's Privacy Advisory Committee, the Secure Anonymised Information Linkage Collaboration Review System, the NHS South-East Scotland Research Ethics Service and The University of Edinburgh's Centre for Population Health Sciences Research Ethics Committee. We will produce a report for Asthma-UK, submit papers to peer-reviewed journals and construct an interactive map

Estimating the Costs of Foundational Public Health Capabilities: A Recommended Methodology

The Institute of Medicine’s 2012 report on public health financing recommended the convening of expert panels to identify the components and costs of a “minimum package of public health services” that should be available in every U.S. community. The report recommended that this minimum package include a core set of public health programs that target specific, high-priority preventable health problems and risks, along with a set of “foundational public health capabilities” that are deemed necessary to support the successful implementation of public health programs and policies. In response to this recommendation, the Robert Wood Johnson Foundation, in collaboration with the US Centers for Disease Control and Prevention and other national professional associations, formed the Public Health Leadership Forum, an expert consensus panel process to identify a recommended set of core programs and foundational capabilities for the nation. The Forum’s initial charge focused on the specification of foundational public health capabilities. The Foundational Capabilities Workgroup was formed as a part of the Forum to identify and define the elements to be included as foundational capabilities for governmental public health agencies at both state and local levels. The Robert Wood Johnson Foundation asked the National Coordinating Center for Public Health Services and Systems Research based at the University of Kentucky to convene a second expert panel workgroup, the Workgroup on Public Health Cost Estimation, to develop a methodology for estimating the resources required to develop and maintain foundational capabilities by governmental public health agencies at both state and local levels. Working in parallel with the Foundational Capabilities Workgroup, this Cost Estimation Workgroup has considered relevant cost-accounting models and cost estimation methodologies, and reviewed related cost estimation studies, in order to make recommendations on an approach for generating first-generation estimates of the costs associated with developing and maintaining foundational capabilities

Empirical Evaluation of the Difficulty of Finding a Good Value of k for the Nearest Neighbor

As an analysis of the classification accuracy bound for the Nearest Neighbor technique, in this work we have studied if it is possible to find a good value of the parmeter k for each example according to their attribute values. Or at least, if there is a pattern for the parameter k in the original search space. We have carried out different approaches based onthe Nearest Neighbor technique and calculated the prediction accuracy for a group of databases from the UCI repository. Based on the experimental results of our study, we can state that, in general, it is not possible to know a priori a specific value of k to correctly classify an unseen example

Recommendations for Urine and Urinary Bladder Collection in Chemical Carcinogenesis Assays with Rodents

This review describes the technical procedures to collect and process urine and urinary bladder samples, during and at the end of urinary bladder carcinogenesis assays with small rodents. The applications, advantages and disadvantages of each method are also mentioned and discussed

Epidemiology of Aortic Aneurysm Repair in the United States from 1993 to 2003

The epidemiology of abdominal aortic aneurysm (AAA) disease has been well described over the preceding 50 years. This disease primarily affects elderly males with smoking, hypertension, and a positive family history contributing to an increased risk of aneurysm formation. The aging population as well as increased screening in high-risk populations has led some to suggest that the incidence of AAAs is increasing. The National Inpatient Sample (1993 2003), a national representative database, was used in this study to determine trends in mortality following AAA repair in the United States. In addition, the impact of the introduction of less invasive endovascular AAA repair was assessed. Overall rates of treated unruptured and ruptured AAAs remained stable (unruptured 12 to 15 100,000; ruptured 1 to 3 100,000). In 2003, 42.7 of unruptured and 8.8 of ruptured AAAs were repaired through an endovascular approach. Inhospital mortality following unruptured AAA repair continues to decline for open repair (5.3 to 4.7 , P 0.007). Mortality after elective endovascular AAA repair also has statistically decreased (2.1 to 1.0 , P 0.024) and remains lower than open repair. Mortality rates for ruptured AAAs following repair remain high (open: 46.5 to 40.7 , P 0.01; endovascular: 40.0 to 35.3 , P 0.823). These data suggest that the numbers of patients undergoing elective AAA repair have remained relatively stable despite the introduction of less invasive technology. A shift in the treatment paradigm is occurring with a higher percentage of patients subjected to elective endovascular AAA repair compared to open repair. This shift, at least in the short term, appears justified as the mortality in patients undergoing elective endovascular AAA repair is significantly reduced compared to patients undergoing open AAA repair.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73855/1/annals.1383.030.pd