Effect of high hydrostatic pressure and temperature on carrot peroxidase inactivation

The combined effects of pressure and temperature on the activity of carrot peroxidase (POD) were investigated in the pressure range 0.1-600 MPa and the temperature range 25-45degreesC. At lower pressures (<396 MPa), carrot POD stability increased compared to unpressurized samples. Inactivation of 91% was obtained at 600 MPa and 45degreesC. High hydrostatic pressure (HHP) combined with temperature treatment enhanced the inactivation of carrot POD. Regeneration of POD activity with the combined HHP and temperature treatments followed first order kinetics at 25, 35 and 40degreesC. Regeneration was not observed at 506 MPa and 45degreesC. HHP had no significant effect on the loss of vitamin C or on protein content. HHP combined with mild heat treatment was found to be better than the thermal treatment at high temperatures for inactivation of POD in carrot processing

Utilization of products obtained from copyrolysis of oil shale and plastic

The main objective of the present study was to investigate pyrolysis of oil shale with polyethylene, in terms of yields and properties of the products obtained. A detailed characterization of gas, oil and char from copyrolysis is presented. Although no synergetic effect on the product yield was observed during the copyrolysis, the addition of polyethylene (PE) to oil shale improved fuel properties of shale oil leading to a decrease in the oxygen content of shale oil. As the ratio of polyethylene increased in the blends, the amount of aliphatic compounds in tars increased while that of polar compounds decreased. In addition, the presence of PE in the blend improved the composition of pyrolysis gas leading to an increase in combustible gases. The production of activated carbon from char obtained from oil shale/polyethylene (1/1) was also carried out. Although the surface area of activated carbon was smaller than that of obtained from lignocellulosic materials, it had a notable adsorption capacity for Cr(VI) (55.25 mg/g). © 2009 Estonian Academy Publishers

Removal of some textile dyes from aqueous solution by using a catalase-peroxidase from aeribacillus pallidus (P26)

The textile dyeing wastewater is discharged to the environment in various occasions and causes severe environmental problems. Therefore, more effective treatment methods should be developed to eliminate dyes from the wastewater stream at the source. In this study, catalase-peroxidase enzyme was purified from Aerobacillus pallidus (P26) isolated from the Erzurum-Pasinler spring (Gen Bank NO: EU935591). A catalase-peroxidase (CP) using enzyme was purified by ammonium sulphate precipitation, CM-Sephadex ion chromatography and Sephacryl S 200 gel filtration chromatography changing. Optimum pH and temperature values were determined as 7 and 60 C degrees, respectively. In addition, for each substrate pairs, K-m and V-max values were calculated from Lineweaver-Burk graphs. The effect of some metal ions was also investigated on the activity of CP enzyme. Purified catalase-peroxidase from A. pallidus (P26) is then applied in the decolorization of Reactive Black 5, Fuchsine, Allura Red and Acid Red 37 in waste water. CP presents a potential as a viable alternative in the decolorization of textile wastewaters