Subacute Oral Toxicity Assessment of Alchornea cordifolia (Schumach and Thonn) Müll Arg (Euphorbiaceae) Extract in Rats

Purpose: To assess Alchornea cordifolia, a medicinal plant with numerous biological actions and uses in traditional medicine, for possible toxicity in rats.Methods: The probable effect of the ethanol extract of Alchornea cordifolia (250 - 2000 mg/kg, p.o.) by gavage was evaluated on blood cellular elements and chemistry, as well as on the weight and histology of vital organs of male adult Spraque-Dawley rats.Results: Daily administration of the extract for two weeks did not cause significant changes in most haematological indices and blood chemistry. However, a dose-dependent increase (p < 0.01) in neutrophils was observed. Relative organ weights were comparable in control and treated groups. Histopathological assessment of liver sections of treated-rats showed normal architecture at doses < 1000 mg/kg. However, in animals treated with 1000 and 2000 mg/kg, cloudy swelling of hepatocytes with vacuolar and hydropic degeneration were evident. Kidney architecture at all dose levels was normal.Conclusion: The results of the study show that administration of the ethanol extract of Alchornea cordifolia to male adult rats by gavage evoked histopathologic changes in the liver at doses > 1000 mg/kg. These findings call for caution in the use of Alchornea cordifolia especially in high doses.Keywords: Alchornea cordifolia, Rats, Subacute oral toxicity, Neutrophils, Hepatocytes, Hydropic denegeratio

The 5-HT2A Receptor Antagonist M100907 Produces Antiparkinsonian Effects and Decreases Striatal Glutamate

5-HT plays a regulatory role in voluntary movements of the basal ganglia and has a major impact on disorders of the basal ganglia such as Parkinson's disease (PD). Clinical studies have suggested that 5-HT2 receptor antagonists may be useful in the treatment of the motor symptoms of PD. We hypothesized that 5-HT2A receptor antagonists may restore motor function by regulating glutamatergic activity in the striatum. Mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) exhibited decreased performance on the beam-walking apparatus. Peripheral administration of the 5-HT2A receptor antagonist M100907 improved performance of MPTP-treated mice on the beam-walking apparatus. In vivo microdialysis revealed an increase in striatal extracellular glutamate in MPTP-treated mice and local perfusion of M100907 into the dorsal striatum significantly decreased extracellular glutamate levels in saline and MPTP-treated mice. Our studies suggest that blockade of 5-HT2A receptors may represent a novel therapeutic target for the motor symptoms of PD

Review of critical success factors for the implementation of total quality management in the construction industry

Abstract: Total Quality Management (TQM) is a philosophy that involves every organization in the industry in the effort to improve performance. The concept is an integrative management principle for continuously improving the quality of products and processes to achieve customer satisfaction. To achieve this concept required a clear establishment of Critical Success Factors (CSFs). However, lack of clear establishment of CSFs for the implementation of TQM in the construction industry has led to failure of the system. In the pursuit of performance excellence in the construction industry and with an increasing awareness of construction quality, construction firms have no option than to implement TQM with clear CSFs

Antibiotic stewardship and its impact on antibiotic use at the child health directorate of a teaching hospital in Ghana

Purpose: The study was intended to ascertain the existence of an antibiotic stewardship programme at the Child Health Directorate of Komfo Anokye Teaching Hospital (KATH) and assess the knowledge of health professionals about the importance of the antimicrobial stewardship programme. Again, it sort to assess the experience of participants with pharmacotherapy of infections and patient outcomes with generic and innovative brands of antibiotics. The third was to assess reporting on adverse reactions and storage conditions of the antibiotics.Design/Methodology/ Approach: This was a cross-sectional study involving health professionals who participated in the provision of infectious disease management services. Eighty-eight (88) professionals were involved in the study, comprising nurses (n=51), medical doctors (n=21), pharmacists (n=8), biomedical scientists (n=5) and public health officers (n=3).Findings: The majority (75 %, n=66) reported treatment failure with generic brands of antibiotics compared to innovator brands. Thirty-four percent of the participants reported adverse drug reactions (ADRs) on antibiotic therapy to superiors instead of filling out ADR forms.Research Limitations: The study was done in one Directorate in the hospital and thus cannot be generalized to reflect the situation in all teaching hospitals in Ghana.Practical implication: The evidence obtained highlighted the need for pragmatic antimicrobial stewardship (AMS) at the directorate to help optimize the management of childhood infections and minimize the emergence and spread of antibiotic resistance.Originality/Value. This was an original project designed to generate evidence to inform interventions to promote the responsible use of antimicrobials in children

Trichilia monadelpha Bark Extracts Inhibit Carrageenan-Induced Foot-Oedema in the 7-Day Old Chick and the Oedema Associated with Adjuvant-Induced Arthritis in Rats

Trichilia monadelpha (Thonn) JJ De Wilde (Meliaceae) bark extract is used in African traditional medicine for the management of various disease conditions including inflammatory disorders such as arthritis. The present study was undertaken to evaluate the anti-inflammatory properties of aqueous (TWE), alcoholic (TAE) and petroleum ether extract (TPEE) of T. monadelpha using the 7-day old chick-carrageenan footpad oedema (acute inflammation) and the adjuvant-induced arthritis model in rats (chronic inflammation). TWE and TPEE significantly inhibited the chick-carrageenan footpad oedema with maximal inhibitions of 57.79±3.92 and 63.83±12 respectively, but TAE did not. The reference anti-inflammatory drugs (diclofenac and dexamethasone) inhibited the chick-carrageenan-induced footpad oedema, with maximal inhibitions of 64.92±2.03 and 71.85±15.34 respectively. Furthermore, all the extracts and the reference anti-inflammatory agents (diclofenac, dexamethasone, methotrexate) inhibited the inflammatory oedema associated with adjuvant arthritis with maximal inhibitions of 64.41±5.56, 57.04±8.57, 62.18±2.56%, for TWE, TAE and TPEE respectively and 80.28±5.79, 85.75±2.96, 74.68±3.03% for diclofenac, dexamethasone and methotrexate respectively. Phytochemical screening of the plant bark confirmed the presence of a large array of plant constituents such as alkaloids, glycosides, flavonoids, saponins, steroids, tannins and terpenoids, all of which may be potential sources of phyto-antiinflammatory agents. In conclusion, our work suggests that T. monadelpha is a potential source of antiinflammatory agents.Keywords: Antiinflammatory, Arthritis, Trichilia monadelpha, chick-carrageenan, phyto-antiinflammatory

Involvement of young people in a qualitative study that seeks to explore experiences of renal replacement therapy choice selection

Introduction: Patient and Public involvement (PPI) has become one of the drivers in health care and seen as an integral part of the research process. PPI promotes research questions that reflect the priorities and concerns of the relevant population and develops outcomes that are important to patients (INVOLVE, 2012; Evans et al, 2014; Hickey et al, 2018). Young adults with end-stage kidney disease are supported by clinicians and their families to make decisions about their preferred renal replacement therapy (NICE, 2018). However, they can become overwhelmed with the burden of long-term chronic illness and often struggle to accept diagnosis and prognosis and cope with the complex management of their condition (Coulter and Collins, 2011; Ritchie et al., 2012). They can consequently become disengaged with the service and less involved in their care (Ritchie et al, 2012; Robinski et al, 2014). The current study explores young people’s experiences of renal replacement therapy choice selection. The study team has involved young people in the study’s development. Aims and objectives: The aim of the involvement group in this study is to involve the young people in all stages of the research process: ensuring that the research question has the correct focus, that the participant information sheet is clear and that the recruitment strategy is appropriate. In due course, the young people’s group will be involved in qualitative data analysis and in dissemination of findings. Method: The involvement group comprised three people with experiences of dialysis and kidney transplant treatment as children and young adults. They were recruited via existing networks in the research team. A role description on what was to be expected of the group members was discussed, alongside what was expected from the research team (training and travel expenses for example). Communication is via face-to face meetings and also by email. This first face-to-face meeting discussed and shaped the research question, and the possible recruitment process. Further email discussions have explored the participant information sheet, consent forms plus the development of the video and poster advert to aid recruitment. Results: The impact of PPI on the research process is already providing valuable insights into how the research design should be conducted. The first face-to-face meeting with the group highlighted the difficulties that young people face when making choice of renal replacement therapy, especially psychosocial impact and mental well-being, so the impact the choice makes on well-being was added as a secondary research question. The acceptability of the recruitment method (via social media) was discussed and a video advert was developed for the recruitment process. Members also contributed to the review of some of the research documents; participant information sheet, consent forms, posters, research proposal and piloted a validation questionnaire for suitability. Impact: Involvement of patients helped situate the study to meet the current needs of young adults diagnosed with end stage renal disease who are faced with choosing dialysis or kidney transplantation

Manure characteristics of small ruminants fed agro by-products in the guinea savannah agro-ecological zone of Ghana

A 90 day study on evaluating quantity and quality of manure from small ruminants in the Guinea Savannah agro-ecological zone of Ghana was conducted. 36 goats and 36 sheep were random­ly allotted to three dietary treatments - Basal diets of maize stover, cassava and yam peels as Treatment one (T1), T1 supplemented with cowpea residues as Treatment two (T2) and T1 supplemented with groundnut residues as Treatment three (T3) in a randomized complete block design. Feed composition and intake and manure characteristics were assessed and data ana­lyzed statistically using Generalized Linear Model procedures of SAS at 5% significance level. The Chemical composition of feed resources ranged from 3.2-17.3% crude protein, 82-97.2% organic matter and 2.8-18% ash. Total feed intake ranged from 519.55-659.72 g/day and 331.84-420.17 g/day for sheep and goats respectively. Sheep manure weighed 319.17-423.33 g/day and contained 20.74-29.01% carbon, 1.87-2.32% nitrogen, 0.77-3.00 potassium, 9.52-26.50% ash, 0.44-0.77 pH and C:N ratio of 10.98-15.53. Goat manure weighed 178.67-216.17 g/day with 19.58-30.61%, 1.56-2.21% nitrogen, 0.82-2.33% potassium, 10.75-19.80% ash, 0.37-1.47 pH and C:N ratio of 9.49-19.92. Results indicated that manure from small ruminants could serve as alternative source of fertilizer in Ghana since its characteristics compare well with chemical fertilizers. Keywords: sheep; goats; feed resources; manure; fertilizer

Anti-inflammatory and antioxidant properties of the root extract of Carissa edulis (forsk.) Vahl (apocynaceae)

Root extracts of Carissa edulis (Forsk.) Vahl (Apocynaceae) are used for the treatment several pathological states including inflammatory disorders. The present study was undertaken to evaluate the effect of an alcoholic extract of C. edulis (CEE) on carrageenan-induced foot oedema in chicks. Also since free radicals and reactive oxygen species are implicated in inflammatory diseases, the anti-oxidant potential of extract was investigated in in vitro experimental models. Oral administration of CEE (30- 300 mg kg-1 p.o.) significantly inhibited carrageenan-induced foot oedemas with a maximal inhibition of 53.8±8.2%. Similarly, the NSAID diclofenac (10-100 mg kg-1, i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3-3 mg kg-1, i.p ) reduced the total oedema with a maximal inhibition of 62.7±9.1% and 66.4±7.8% respectively. The extract also scavenged DPPH and prevented lipid peroxidation in rat brain homogenates. These results suggest that alcoholic extract of C. edulis exerts in vivo antiinflammatory activity after oral administration and also has antioxidant properties which may contribute to its activity.Journal of Science & Technology (Ghana) Vol. 27 (2) 2007: pp. 6-1