205 research outputs found
Dynamic PRA: an Overview of New Algorithms to Generate, Analyze and Visualize Data
State of the art PRA methods, i.e. Dynamic PRA
(DPRA) methodologies, largely employ system
simulator codes to accurately model system dynamics.
Typically, these system simulator codes (e.g., RELAP5 )
are coupled with other codes (e.g., ADAPT,
RAVEN that monitor and control the simulation. The
latter codes, in particular, introduce both deterministic
(e.g., system control logic, operating procedures) and
stochastic (e.g., component failures, variable uncertainties)
elements into the simulation. A typical DPRA analysis is
performed by:
1. Sampling values of a set of parameters from the
uncertainty space of interest
2. Simulating the system behavior for that specific set of
parameter values
3. Analyzing the set of simulation runs
4. Visualizing the correlations between parameter values
and simulation outcome
Step 1 is typically performed by randomly sampling
from a given distribution (i.e., Monte-Carlo) or selecting
such parameter values as inputs from the user (i.e.,
Dynamic Event Tre
Assessment of the angiogenic potential of 2-deoxy-D-ribose using a novel in vitro 3D dynamic model in comparison with established in vitro assays
Angiogenesis is a highly ordered physiological process regulated by the interaction of endothelial cells with an extensive variety of growth factors, extracellular matrix components and mechanical stimuli. One of the most important challenges in tissue engineering is the rapid neovascularization of constructs to ensure their survival after transplantation. To achieve this, the use of pro-angiogenic agents is a widely accepted approach. The study of angiogenesis has gained momentum over the last two decades. Although there are various in vitro, ex vivo, and in vivo angiogenesis models that enable testing of newly discovered pro-angiogenic agents, the problem with researching angiogenesis is the choice of the most appropriate assay. In vivo assays are the most representative and reliable models, but they are expensive, time-consuming and can cause ethical concerns whereas in vitro assays are relatively inexpensive, practical, and reproducible, but they are usually lack of enabling the study of more than one aspect of angiogenesis, and they do not fully represent the complexity of physiological angiogenesis. Therefore, there is a need for the development of an angiogenesis model that allows the study of angiogenesis under physiologically more relevant, dynamic conditions without causing ethical concerns. Accordingly, in this study, we developed 3D in vitro dynamic angiogenesis model, and we tested the angiogenic potential of 2-deoxy-D-ribose (2dDR) in comparison with vascular endothelial growth factor (VEGF) using newly developed in vitro 3D dynamic model and well-established in vitro models. Our results obtained using conventional in vitro assays demonstrated that 2dDR promoted proliferation, migration and tube formation of human aortic endothelial cells (HAECs) in a dose-dependent manner. Then, the angiogenic activity of 2dDR was further assessed using the newly developed 3D in vitro model, which enabled the monitoring of cell proliferation and infiltration simultaneously under dynamic conditions. Our results showed that the administration of 2dDR and VEGF significantly enhanced the outgrowth of HAECs and the cellular density under either static or dynamic conditions
Preparation of interconnected Pickering polymerized high internal phase emulsions by arrested coalescence
Emulsion templating is a method that enables the production of highly porous and interconnected polymer foams called polymerized high internal phase emulsions (PolyHIPEs). Since emulsions are inherently unstable systems, they can be stabilized either by surfactants or by particles (Pickering HIPEs). Surfactant-stabilized HIPEs form materials with an interconnected porous structure, while Pickering HIPEs typically form closed pore materials. In this study, we describe a system that uses submicrometer polymer particles to stabilize the emulsions. Polymers fabricated from these Pickering emulsions exhibit, unlike traditional Pickering emulsions, highly interconnected large pore structures, and we related these structures to arrested coalescence. We describe in detail the morphological properties of this system and their dependence on different production parameters. This production method might provide an interesting alternative to poly-surfactant-stabilized-HIPEs, in particular where the application necessitates large pore structures
In vivo bone regeneration capacity of multiscale porous polycaprolactone-based high internal phase emulsion (PolyHIPE) scaffolds in a rat calvarial defect model
Globally, one of the most common tissue transplantation procedures is bone grafting. Lately, we have reported the development of polymerized high internal phase emulsions (PolyHIPEs) made of photocurable polycaprolactone (4PCLMA) and shown their potential to be used as bone tissue engineering scaffolds in vitro. However, it is essential to evaluate the in vivo performance of these scaffolds to investigate their potential in a clinically more relevant manner. Therefore, in this study, we aimed to compare in vivo performances of macroporous (fabricated using stereolithography), microporous (fabricated using emulsion templating), and multiscale porous (fabricated using emulsion templating and perforation) scaffolds made of 4PCLMA. Also, 3D-printed macroporous scaffolds (fabricated using fused deposition modeling) made of thermoplastic polycaprolactone were used as a control. Scaffolds were implanted into a critical-sized calvarial defect, animals were sacrificed 4 or 8 weeks after implantation, and the new bone formation was assessed by micro-computed tomography, dental radiography, and histology. Multiscale porous scaffolds that include both micro- and macropores resulted in higher bone regeneration in the defect area compared to only macroporous or only microporous scaffolds. When one-grade porous scaffolds were compared, microporous scaffolds showed better performance than macroporous scaffolds in terms of mineralized bone volume and tissue regeneration. Micro-CT results revealed that while bone volume/tissue volume (Bv/Tv) values were 8 and 17% at weeks 4 and 8 for macroporous scaffolds, they were significantly higher for microporous scaffolds, with values of 26 and 33%, respectively. Taken together, the results reported in this study showed the potential application of multiscale PolyHIPE scaffolds, in particular, as a promising material for bone regeneration
Cell therapy in pulmonary diseases: are there perspectives?
A terapia celular poderia ser conceituada de forma ampla e genérica como o emprego de células para tratamento de doenças. Apesar de um número não tão expressivo de relatos tendo o pulmão como objeto de estudo na terapia celular em pacientes humanos, há dados consistentes da literatura, tanto em humanos, quanto em modelos animais,que evidenciam a migração de células-tronco da medula óssea para o pulmão,em diferentes situações experimentais. Esses resultados forneceram o embasamento experimental para o emprego de células-tronco na regeneração do tecido pulmonar em modelos animais. Em nosso laboratório, vários projetos de pesquisa têm sido conduzidos com a finalidade de avaliar a resposta pulmonar (morfológica e funcional) ao tratamento com células-tronco adultas em camundongos com doença pulmonar obstrutiva crônica (DPOC) induzida experimentalmente. Os resultados obtidos, aliados à queles de outros grupos de pesquisa, permitem aventar a possibilidade de aplicação, a curto prazo, da terapia celular em pacientes com DPOC. Em outra patologia pulmonar, fibrose cÃstica (FC), cuja abordagem terapêutica com células-tronco apresenta aspectos particulares em relação à s patologias pulmonares crônico-degenerativas, há avanços promissores e potencialmente interessantes; no entanto, os resultados podem ser considerados incipientes e deve-se assinalar, portanto, que a associação da terapia gênica e celular apresenta-se como uma alternativa possÃvel, mas ainda muito distante quanto à sua consolidação e incorporação como opção terapêutica segura e eficaz em FC. Por outro lado, tendo por embasamento os resultados obtidos em modelos experimentais, é possÃvel postular que a terapia celular com células-tronco hematopoéticas (ou de outras fontes) encerra perspectivas consistentes de aplicação em diversas outras patologias pulmonares humanas, especialmente em DPOC.Cell therapy can be briefly described as the use of cells in the treatment of diseases. Although the number of scientific reports involving lung and cell therapy in humans is not expressive, there are consistent data, both in humans and animal models. Experiments show the migration of bone marrow stem cells to the lung, in different situations. These results provide the experimental basis for the use of stem cells in the regeneration of the lung tissue in animal models. In our laboratory, several projects have been conducted aiming to evaluate the pulmonary response (morphological and functional) to treatment with adult stem cells in mice with experimentally induced chronic obstructive pulmonary disease. The results obtained, together with those from other research groups, allow us to consider the possibility of application, in the near future, of cell therapy in chronic obstructive pulmonary disease patients. For another disease, cystic fibrosis, cell therapy shows particular aspects in relation to other chronic degenerative pulmonary diseases. In this pathology, there are interesting and promising advances, however, the results are incipient and, thus, it must be said that the association between genetic and cell therapy appears to be a possibility, but still far from being consolidated and incorporated as a safe and effective therapeutic alternative in cystic fibrosis. On the other hand, based on the results obtained in experimental models, it is possible to postulate that cell therapy with hematopoietic stem cells (or from other sources) brings consistent application perspectives in several other human pulmonary diseases, especially in chronic obstructive pulmonary disease.CNPqFundunesp Fundação BiomaVal
A novel bilayer polycaprolactone membrane for guided bone regeneration : combining electrospinning and emulsion templating
Guided bone regeneration is a common dental implant treatment where a barrier membrane (BM) is used between epithelial tissue and bone or bone graft to prevent the invasion of the fast-proliferating epithelial cells into the defect site to be able to preserve a space for infiltration of slower-growing bone cells into the periodontal defect site. In this study, a bilayer polycaprolactone (PCL) BM was developed by combining electrospinning and emulsion templating techniques. First, a 250 µm thick polymerised high internal phase emulsion (polyHIPE) made of photocurable PCL was manufactured and treated with air plasma, which was shown to enhance the cellular infiltration. Then, four solvent compositions were investigated to find the best composition for electrospinning a nanofibrous PCL barrier layer on PCL polyHIPE. The biocompatibility and the barrier properties of the electrospun layer were demonstrated over four weeks in vitro by histological staining. Following in vitro assessment of cell viability and cell migration, cell infiltration and the potential of PCL polyHIPE for supporting blood vessel ingrowth were further investigated using an ex-ovo chick chorioallantoic membrane assay. Our results demonstrated that the nanofibrous PCL electrospun layer was capable of limiting cell infiltration for at least four weeks, while PCL polyHIPE supported cell infiltration, calcium and mineral deposition of bone cells, and blood vessel ingrowth through pores
Experimentação e uso regular de drogas ilÃcitas por estudantes no municÃpio de Bragança, nordeste do Pará
Este estudo determinou a prevalência e os fatores associados ao uso de drogas ilÃcitas entre estudantes de escolas de ensino médio do municÃpio de Bragança, Pará, norte do Brasil. Estudo transversal de base escolar, totalizando cinco escolas públicas e 1.632 estudantes do municÃpio de Bragança. O questionário auto-aplicável foi adaptado de outros estudos epidemiológicos adequados ao grupo populacional, com rigoroso procedimento, garantindo anonimato e sigilo. A prevalência de uso de drogas ilÃcitas entre estudantes foi 4,37%. Diversos fatores de risco ao uso de drogas lÃcitas e ilÃcitas foram detectados, dos quais destacam-se: a ausência de participação dos pais na vida escolar dos estudantes, renda familiar superior a 3 salários mÃnimos, pais, amigos e familiares que usam droga ilÃcita e a falta de incentivo para não usar droga ilÃcita. Em suma, este estudo identificou que o uso regular de drogas ilÃcitas está associada a fatores sociais e econômicos, os quais deverão ser considerados na elaboração de medidas para controle e prevenção ao uso de drogas ilÃcitas no municÃpio de Bragança
Identification of quantitative trait loci associated with iron deficiency chlorosis resistance in groundnut ( Arachis hypogaea )
Iron deficiency chlorosis is an important abiotic stress affecting groundnut production
worldwide in calcareous and alkaline soils with a pH of 7.5–8.5. To identify genomic
regions controlling iron deficiency chlorosis resistance in groundnut, the recombinant
inbred line population from the cross TAG 24 × ICGV 86031 was evaluated for associated
traits like visual chlorosis rating and SPAD chlorophyll meter reading across
three crop growth stages for two consecutive years. Thirty-two QTLs were identified
for visual chlorosis rating (3.9%–31.8% phenotypic variance explained [PVE]) and
SPAD chlorophyll meter reading [3.8%–11% PVE] across three stages over 2 years.
This is the first report of identification of QTLs for iron deficiency chlorosis resistance-
associated traits in groundnut. Three major QTLs (>10% PVE) were identified at
severe stage, while majority of other QTLs were having small effects. Interestingly,
two major QTLs for visual chlorosis rating at 60 days (2013) and 90 days (2014) were
located at same position on LG AhXIII. The identified QTLs/markers after validation
across diverse genetic material could be used in genomics-assisted breeding
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