Well-Being of Lesbian, Gay, Bisexual Youth: The Influence of Rural and Urban Contexts on the Process of Building Identity and Disclosure

The study investigates how the territorial community can influence the individual and social well-being of lesbian, gay, bisexual (LGB) youth and especially the recognition of their feelings and the construction of their own identity as well as their needs to be socially recognized. This research focuses on the experiences of 30 LGB individuals (23 males and 7 females), with a mean age of 25.07 years (SD = 4,578), living in urban and rural areas of Southern Italy. Focalized open interviews were conducted, and the Grounded Theory Methodology, supported by the Atlas.ti 8.0 software, was used for data analysis. The textual material was first coded, and then codes were grouped into five macro-categories: Freedom of identity expression in the urban and rural context, identity construction and acceptance process, need of aggregation and identification with the LGB community, role of the interpersonal relationship in the process of identity acceptance, socio-cultural context, and LGB psychological well-being. The results showed a condition common to the two contexts that we can define as “ghettoization.” The young LGB is alone in the rural area due to a lack of places and people to identify with and greater social isolation. On the contrary, although there are more opportunities in the urban area, young people feel stigmatized and ghettoized because “their places” are frequented exclusively by the lesbian, gay, bisexual, transexual, queer (LGBTQ) community. The work will extensively discuss the limitations of the research, future proposals, and the practical implications of the results

Psychological and overall well-being of Italian young adults in transition to adulthood: Evidence from a sequential explanatory mixed-methods study

YesTransitioning to adulthood is a fundamental yet challenging phase in human development. Despite its relevance, the literature has paid little attention to the impact that transitioning to adulthood has on multiple aspects of individual well-being. This is one of the few attempts that has employed a sequential explanatory mixed-methods study to explore how the path to adulthood impacted on the psychological and overall well-being of Italian young adults. In the quantitative phase, we employed two-step cluster analysis to assign 45 young adults, who had completed the Italian I COPPE scale of multidimensional well-being, to three clusters based on their level of change in psychological and overall well-being. Results from mixed design ANCOVA revealed that participants belonging to the high change cluster showed the highest significant positive change over time with respect to well-being. In the qualitative phase, 17 individuals took part in focalized narrative interviews, which aimed at exploring significant events, barriers, and facilitators, which had impacted on their well-being during the transition to adulthood. The textual material was analysed through the Grounded Theory Methodology. It emerged that the state of transition was associated with some triggering events and a temporary negative impact on the interviewees' well-being. However, findings also revealed that people assigned to different clusters had put in place or learned several resources, skills, and copying strategies, which led to different transitional and well-being outcomes

Contextual influences on italian university students during the covid-19 lockdown: Emotional responses, coping strategies and resilience

none17Based on an ecological perspective on the COVID-19 lockdown experience, this study describes psychological responses among Italian university students. Our study considers three zones of the country that have differed in the intensity of the COVID-19 pandemic. Specifically, this research explores whether differences in pandemic conditions can account for their divergent psychological outcomes. The participants were 792 university students from seven different Italian universities. Students were asked to express their emotions and describe meaningful events during the lockdown in writing. Based on the grounded theory approach, this study conducted qualitative data analysis using ATLAS.ti 8.0. The core emerged categories are emotions, emotional moods and state of mind, coping strategies, and resilience. The results describing these emergent factors in relation to environmental variables highlight differences in the feeling of anxiety among individuals: anxiety was more self-focused in zones that were more affected by the lockdown, while such anxiety was more related to family and friends in less-affected zones. In addition to identifying the negative repercussions that this emergency has had, this study describes some positive outcomes, such as the elaboration of new personal perspectives that help foster individual growth and allow individuals to gain new awareness of themselves and others. The confinement due to the COVID-19 emergency measures has been a very unique experience for people, and further research is needed to understand the long-term effects of the different coping responses activated by participants during and after the lockdown.mixedLaura Migliorini; Norma De Piccoli; Paola Cardinali; Chiara Rollero; Daniela Marzana; Caterina Arcidiacono; Elisa Guidi; Ciro Esposito; Cinzia Novara; Angela Fedi; Elena Marta; Andrea Guazzini; Patrizia Meringolo; Maria Grazia Monaci; Barbara Agueli; Fortuna Procentese; Immacolata Di NapoliMigliorini, Laura; De Piccoli, Norma; Cardinali, Paola; Rollero, Chiara; Marzana, Daniela; Arcidiacono, Caterina; Guidi, Elisa; Esposito, Ciro; Novara, Cinzia; Fedi, Angela; Marta, Elena; Guazzini, Andrea; Meringolo, Patrizia; Grazia Monaci, Maria; Agueli, Barbara; Procentese, Fortuna; Di Napoli, Immacolat

Remote working during the COVID-19 pandemic: A study on the emotional and relational experience and on the well-being of Italian university scholars and clerks

The COVID-19 pandemic has had significant effects on people's well-being and also on their ordinary work activities. This study aims to investigate the emotions, relational experience and well-being of academic personnel who continued their activities in remote working, during the Italian lockdown period in the months of March and April 2020. For this purpose, 87 workers (55 % scholars and 45 % university clerks) filled out an online quali-quantitative questionnaire about their experiences of being in lockdown and doing their work remotely. Qualitative data were analyzed through Grounded Theory Methodology using the ATLAS.ti 8.4 software. From the coding process, the following macro-categories emerged: remote working, affects and interpersonal relationships in lockdown. Then cross-tabs intertwined the code groups emerged with work role and gender of participants. In the end, the frequencies in the cross-tabs were analyzed by Chi square test. Quantitative data were analyzed through Univariate Analysis of Variance (ANOVA) using SPSS 22 software. The results showed that scholars consider remote working mainly as a critical issue, while clerks see it more as a resource. Moreover, clerks more frequently report negative feelings of anxiety, fear and anger, while scholars more frequently report loneliness. Regarding interpersonal relationships, no significant differences between scholars and clerks were observed. Neither affects nor interpersonal relationships were associated with gender differences. Moreover, clerks reported lower economic and overall well-being compared to scholars. Finally, results highlighted the importance of the mattering role of human relations in work activities; this core category gives some practical implications that will be discussed extensively

Localization of HSP70, Cdc2, and cyclin B in sea urchin oocytes in non-stressed conditions

In Paracentrotus lividus embryos, a Mediterranean sea urchin species, HSP70 is present in all the cells. During cell division it localizes under normal growth conditions on the centrosomes and on the whole isolated mitotic apparatus. Now, in situ hybridization, Western blot analyses, and immunohistochemistry show that the HSP70 mRNA is present in both small and large P. lividus oocytes, that all four isoforms of HSP70 can be found also in the oocytes, and that a certain amount of HSP70 localizes on asters and spindles during polar body formation. Moreover, two representative cell-cycle related proteins, cyclin B, and Cdc2, are present both in small and large oocytes, concentrating in the germinal vesicles before its breaking down. Cdc2 has been found in the cytoplasm of small oocytes and in the germinal vesicles of the large ones and then together with HSP70 on the mitotic apparatus of the dividing oocytes. \ua9 2003 Elsevier Inc. All rights reserved

Well-Being and the COVID-19 Pandemic: A Community Psychology Systematic Review

The purpose of this systematic review is to analyze the COVID-19 pandemic s effects through the lens of community psychology. Our study applied PRISMA methodology, and it was carried out using the PsycInfo, PubMed, Embase, and EBSCO databases utilizing the keywords "connectedness," "resilience," "shared action," "social action," "trust," "individual well-being," and "social well-being." We selected 12 of 216 eligible publications based on the inclusion criteria. The results indicated that the role of trust in institutions and others is a protective factor for communities that experience crises, such as the COVID-19 pandemic. Moreover, developing social actions promoted connections and social support and that, in the absence of in-person communication, online communication promoted connections and reduced psychological distress and isolation. Finally, the study revealed that some psychosocial factors stem from declines in people s well-being due to the pandemic and subsequent lockdowns. Furthermore, our findings highlighted the role of technology as a positive communication tool for promoting connectedness and social support, as well as the social function of trust in institutions. Our results suggested that community interactions at the local and virtual levels should be viewed as positive actions to support institutions in detecting the most effective social intervention measures to stimulate individual and community well-being during the pandemic. These findings may serve as a guide for health policymakers in their efforts to face the effects of the COVID-19 pandemic supporting beneficial strategies for social planning and effective public policies. Finally, both the strengths and limitations of the research will be discussed in depth

Psychological and overall well-being of Italian young adults in transition to adulthood: Evidence from a sequential explanatory mixed-methods study

Transitioning to adulthood is a fundamental yet challenging phase in human development. Despite its relevance, the literature has paid little attention to the impact that transitioning to adulthood has on multiple aspects of individual well-being. This is one of the few attempts that has employed a sequential explanatory mixed-methods study to explore how the path to adulthood impacted on the psychological and overall well-being of Italian young adults. In the quantitative phase, we employed two-step cluster analysis to assign 45 young adults, who had completed the Italian I COPPE scale of multidimensional well-being, to three clusters based on their level of change in psychological and overall well-being. Results from mixed design ANCOVA revealed that participants belonging to the high change cluster showed the highest significant positive change over time with respect to well-being. In the qualitative phase, 17 individuals took part in focalized narrative interviews, which aimed at exploring significant events, barriers, and facilitators, which had impacted on their well-being during the transition to adulthood. The textual material was analysed through the Grounded Theory Methodology. It emerged that the state of transition was associated with some triggering events and a temporary negative impact on the interviewees' well-being. However, findings also revealed that people assigned to different clusters had put in place or learned several resources, skills, and copying strategies, which led to different transitional and well-being outcomes

A constitutive 70 kDa heat-shock protein is localized on the fibres of spindles and asters at metaphase in an ATP-dependent manner: a new chaperone role is proposed.

In the present study, double immunofluorescence and immunoblot analysis have been used to show that centrosomes, isolated from Paracentrotus lividus sea urchin embryos at the first mitotic metaphase, contain the constitutive chaperone, heat-shock protein (HSP) 70. More specifically, we demonstrate that centrosomes contain only the HSP70-d isoform, which is one of the four isoforms identified in P. lividus. We also provide evidence that p34(cell division control kinase-2) and t complex polypeptide-1 (TCP-1) alpha, a subunit of the TCP-1 complex, are localized on the centrosomes. Furthermore, inhibition of TCP-1 in vivo, via microinjecting an anti-(TCP-1 alpha) antibody into P. lividus eggs before fertilization, either impaired mitosis or induced severe malformations in more than 50% of embryos. In addition, we have isolated the whole mitotic apparatus and shown that HSP70 localizes on the fibres of spindles and asters, and binds them in an ATP-dependent manner. These observations suggest that HSP70 has a chaperone role in assisting the TCP-1 complex in tubulin folding, when localized on centrosomes, and during the assembling and disassembling of the mitotic apparatus, when localized on the fibres of spindles and asters