Constitutive Association of Tie1 and Tie2 with Endothelial Integrins is Functionally Modulated by Angiopoietin-1 and Fibronectin

Functional cross-talk between Tie2 and Integrin signaling pathways is essential to coordinate endothelial cell adhesion and migration in response to the extracellular matrix, yet the mechanisms behind this phenomenon are unclear. Here, we examine the possibility that receptor cross-talk is driven through uncharacterized Tie-integrin interactions on the endothelial surface. Using a live cell FRET-based proximity assay, we monitor Tie-integrin receptor recognition and demonstrate that both Tie1 and Tie2 readily associate with integrins α5ß1 and αVß3 through their respective ectodomains. Although not required, Tie2-integrin association is significantly enhanced in the presence of the extracellular component and integrin ligand fibronectin. In vitro binding assays with purified components reveal that Tie-integrin recognition is direct, and further demonstrate that the receptor binding domain of the Tie2 ligand Ang-1, but not the receptor binding domain of Ang-2, can independently associate with α5ß1 or αVß3. Finally, we reveal that cooperative Tie/integrin interactions selectively stimulate ERK/MAPK signaling in the presence of both Ang-1 and fibronectin, suggesting a molecular mechanism to sensitize Tie2 to extracellular matrix. We provide a mechanistic model highlighting the role of receptor localization and association in regulating distinct signaling cascades and in turn, the angiogenic switch

Synchronized pulse control of decoherence

We present a new strategy for multipulse control over decoherence. When a two-level system interacts with a reservoir characterized by a specific frequency, we find that the decoherence is effectively suppressed by synchronizing the pulse-train application with the dynamical motion of the reservoir.Comment: 14 pages, 8 figure

The Effect of Hot Gas in WMAP's First Year Data

By cross-correlating templates constructed from the 2 Micron All Sky Survey (2MASS) Extended Source (XSC) catalogue with WMAP's first year data, we search for the thermal Sunyaev-Zel'dovich signature induced by hot gas in the local Universe. Assuming that galaxies trace the distribution of hot gas, we select regions on the sky with the largest projected density of galaxies. Under conservative assumptions on the amplitude of foreground residuals, we find a temperature decrement of -35 $\pm$ 7 $\mu$K ($\sim 5\sigma$ detection level, the highest reported so far) in the $\sim$ 26 square degrees of the sky containing the largest number of galaxies per solid angle. We show that most of the reported signal is caused by known galaxy clusters which, when convolved with the average beam of the WMAP W band channel, subtend a typical angular size of 20--30 arcmins. Finally, after removing from our analyses all pixels associated with known optical and X-ray galaxy clusters, we still find a tSZ decrement of -96 $\pm$ 37 $\mu$K in pixels subtending about $\sim$ 0.8 square degrees on the sky. Most of this signal is coming from five different cluster candidates in the Zone of Avoidance (ZoA), present in the Clusters In the ZoA (CIZA) catalogue. We found no evidence that structures less bound than clusters contribute to the tSZ signal present in the WMAP data.Comment: 10 pages, 4 figures, matches accepted version in ApJ Letter

Asymmetric double-well potential for single atom interferometry

We consider the evolution of a single-atom wavefunction in a time-dependent double-well interferometer in the presence of a spatially asymmetric potential. We examine a case where a single trapping potential is split into an asymmetric double well and then recombined again. The interferometer involves a measurement of the first excited state population as a sensitive measure of the asymmetric potential. Based on a two-mode approximation a Bloch vector model provides a simple and satisfactory description of the dynamical evolution. We discuss the roles of adiabaticity and asymmetry in the double-well interferometer. The Bloch model allows us to account for the effects of asymmetry on the excited state population throughout the interferometric process and to choose the appropriate splitting, holding and recombination periods in order to maximize the output signal. We also compare the outcomes of the Bloch vector model with the results of numerical simulations of the multi-state time-dependent Schroedinger equation.Comment: 9 pages, 6 figure

Redshifts and Velocity Dispersions of Galaxy Clusters in the Horologium-Reticulum Supercluster

We present 118 new optical redshifts for galaxies in 12 clusters in the Horologium-Reticulum supercluster (HRS) of galaxies. For 76 galaxies, the data were obtained with the Dual Beam Spectrograph on the 2.3m telescope of the Australian National University at Siding Spring Observatory. After combining 42 previously unpublished redshifts with our new sample, we determine mean redshifts and velocity dispersions for 13 clusters, in which previous observational data were sparse. In six of the 13 clusters, the newly determined mean redshifts differ by more than 750 km/s from the published values. In the case of three clusters, A3047, A3109, and A3120, the redshift data indicate the presence of multiple components along the line of sight. The new cluster redshifts, when combined with other reliable mean redshifts for clusters in the HRS, are found to be distinctly bi-modal. Furthermore, the two redshift components are consistent with the bi-modal redshift distribution found for the inter-cluster galaxies in the HRS by Fleenor et al. (2005).Comment: 13 pages, 3 figures, Accepted to A

Spall And Dynamic Yielding Of Aluminum And Aluminum Alloys At Strain Rates Of 3X10(6) S(-1)

We have explored the role that grain size, impurity particles and alloying in aluminum play in dynamic yielding and spall fracture at tensile strain rates of similar to 3x10(6) We achieved these strain rates shocking the aluminum specimens via laser ablation using the Z-Beamlet Laser at Sandia National Laboratories. The high purity aluminum and 1100 series aluminum alloy produced very different spall strengths and nearly the same yield strengths. In contrast, various grain-sized Al + 3 wt. % Mg specimens presented the lowest spall strength, but the greatest dynamic yield strength. Fracture morphology results and particle analysis are presented along with hydrodynamic simulations to put these results in context. Impurity particles appeared to play a vital role in spall fracture at these fast strain rates. Alloying elements such as Mg seem to be the dominant factor in the dynamic yield results.Mechanical Engineerin

The rodent research animal holding facility as a barrier to environmental contamination

The rodent Research Animal Holding Facility (RAHF), developed by NASA Ames Research Center (ARC) to separately house rodents in a Spacelab, was verified as a barrier to environmental contaminants during a 12-day biocompatibility test. Environmental contaminants considered were solid particulates, microorganisms, ammonia, and typical animal odors. The 12-day test conducted in August 1988 was designed to verify that the rodent RAHF system would adequately support and maintain animal specimens during normal system operations. Additional objectives of this test were to demonstrate that: (1) the system would capture typical particulate debris produced by the animal; (2) microorganisms would be contained; and (3) the passage of animal odors was adequately controlled. In addition, the amount of carbon dioxide exhausted by the RAHF system was to be quantified. Of primary importance during the test was the demonstration that the RAHF would contain particles greater than 150 micrometers. This was verified after analyzing collection plates placed under exhaust air ducts and rodent cages during cage maintenance operations, e.g., waste tray and feeder changeouts. Microbiological testing identified no additional organisms in the test environment that could be traced to the RAHF. Odor containment was demonstrated to be less than barely detectable. Ammonia could not be detected in the exhaust air from the RAHF system. Carbon dioxide levels were verified to be less than 0.35 percent

COVID-19 and Preventing Harm to Vulnerable Children

Although COVID-19 mercifully seems to affect children less severely than adults, children are far from immune from the impacts of the virus. Public health orders closing schools and businesses, cancelling events, and keeping children at home have been disruptive and distressing to many children and families. But for children who rely on government entities for protection, care, custody, and services, the effects of the public health orders can be devastating. COVID-19 and the response to it has serious implications for the safety, well-being, and development of these vulnerable children—those within the child welfare, juvenile justice, and special education systems. All three groups consist of children to whom the state has legal obligations. Additionally, all three groups consist disproportionately of children of color, a reality being brought to the forefront in the context of this pandemic and beyond. As these child-serving systems adapt to the new realities defined by public health limitations, there exists an opportunity to address both immediate challenges as well as enduring concerns within these complex structures. This Article explores the current state of child rights within the child welfare, juvenile justice, and special education systems, highlighting concerns that pre-date COVID-19 as well as recent legal implications of the pandemic. Each section examines the particular repercussions of the pandemic and the response to it on children and proposes potential remedies. It also offers perspective on how meeting today’s critical challenges can result in long-term systemic improvements