Ponderea unor profiluri ale metilării promotorului genei p15 (locus: CDKN2B) la pacienții cu cardiopatie ischemică de origine aterosclerotică

Studiul a avut ca scop stabilirea ponderii unor profiluri ale metilării promotorului genei p15 (locus CDKN2B) în celulele sanguine ale pacienților cu cardiopatie ischemică de origine aterosclerotică prin amplifi carea porţiunilor genice cu ajutorul perechilor de praimeri nonmetil-specifici şi metil-specifici (tehnica MSP). Obiectul studiului l-au constituit 35 de pacienţi și 35 persoane sănătoase. Concluziile: 1) A fost stabilită corespunderea fenotipului cardiopatie ischemică cu metilarea ADN, la nivelul promotorului genei p15 (profilul MM); 2) Celulele analizate UM, au reprezentat un amestec, în care, cele mai multe au conținut ambele molecule de ADN metilate (MM), iar un număr mai mic de celule au inclus fie ambele molecule de ADN nemetilate (UU), fie o moleculă ADN metilată, iar cealaltă – nemetilată (UM)

Utilitatea potenţială a profilurilor metilice ale ADN pentru procesul de promovare a markerilor molecularepigenetici în diagnosticul diferenţial al leucemiilor acute

În cadrul studiului dat au fost obţinute date preliminare privind ponderea grupurilor de pacienţi cu diferite subtipuri de leucemii acute în lotul specifi c de profi l metilic ADN. Am conchis că moleculele nemetilate de ADN, la nivelul promotorului genei p15, prezintă potenţial de marker molecular-epigenetic al leucemiei acute monoblastice la om. Pentru prelucrarea statistică a datelor a fost utilizat testul „U-Fisher”, iar valoarea p≤0,1 a fost interpretată ca tendinţă statistică а diferenţelor dintre grupurile comparate

Endothelial nitric oxide synthase gene polymorphism in patients with acute coronary syndrome

Laboratorul de Genetică USMF „Nicolae Testemiţanu”, IMSP Institutul de CardiologieThe aim was to examine the effect of polymorphism of the endothelial nitric oxide synthase (eNOS) gene, located in the 4a/4b VNTR (variable number of tandem repeats) minisatelit marker of eNOS in patients with acute coronary syndrome. The career of the intron- 4a/4b and 4b/4b polymorphism of the eNOS gene was associated with atherosclerotic coronary lesion and increased risk of myocardial infarction. Larger studies are needed to understand the importance of eNOS gen in the coronary heart disease. Scopul studiului a fost examinarea rolului polimorfismului genei sintetazei endoteliale de oxid nitric (eNOS), localizate pe markerul polimorf minisatelit eNOS-4a/4b VNTR (variable number of tandem repeats) la pacienţii cu sindrom coronarian acut (SCA). Portajul de alelele intron-4a/4b şi 4b/4b VNTR ale eNOS s-a asociat cu lezare aterosclerotică coronariană şi risc crescut de infarct miocardic. Sunt necesare studii largi pentru a înţelege importanţa polimorfismului genei eNOS în boala coronariană

Mapping our Universe in 3D with MITEoR

Mapping our universe in 3D by imaging the redshifted 21 cm line from neutral hydrogen has the potential to overtake the cosmic microwave background as our most powerful cosmological probe, because it can map a much larger volume of our Universe, shedding new light on the epoch of reionization, inflation, dark matter, dark energy, and neutrino masses. We report on MITEoR, a pathfinder low-frequency radio interferometer whose goal is to test technologies that greatly reduce the cost of such 3D mapping for a given sensitivity. MITEoR accomplishes this by using massive baseline redundancy both to enable automated precision calibration and to cut the correlator cost scaling from N^2 to NlogN, where N is the number of antennas. The success of MITEoR with its 64 dual-polarization elements bodes well for the more ambitious HERA project, which would incorporate many identical or similar technologies using an order of magnitude more antennas, each with dramatically larger collecting area.Comment: To be published in proceedings of 2013 IEEE International Symposium on Phased Array Systems & Technolog

CMB-S4 Science Book, First Edition

This book lays out the scientific goals to be addressed by the next-generation ground-based cosmic microwave background experiment, CMB-S4, envisioned to consist of dedicated telescopes at the South Pole, the high Chilean Atacama plateau and possibly a northern hemisphere site, all equipped with new superconducting cameras. CMB-S4 will dramatically advance cosmological studies by crossing critical thresholds in the search for the B-mode polarization signature of primordial gravitational waves, in the determination of the number and masses of the neutrinos, in the search for evidence of new light relics, in constraining the nature of dark energy, and in testing general relativity on large scales

Brute-Force Mapmaking with Compact Interferometers: A MITEoR Northern Sky Map from 128 MHz to 175 MHz

We present a new method for interferometric imaging that is ideal for the large fields of view and compact arrays common in 21 cm cosmology. We first demonstrate the method with the simulations for two very different low-frequency interferometers, the Murchison Widefield Array and the MIT Epoch of Reionization (MITEoR) experiment. We then apply the method to the MITEoR data set collected in 2013 July to obtain the first northern sky map from 128 to 175 MHz at ∼2° resolution and find an overall spectral index of −2.73 ± 0.11. The success of this imaging method bodes well for upcoming compact redundant low-frequency arrays such as Hydrogen Epoch of Reionization Array. Both the MITEoR interferometric data and the 150 MHz sky map are available at http://space.mit.edu/home/tegmark/omniscope.html.National Science Foundation (U.S.) (AST-0908848)National Science Foundation (U.S.) (AST-1105835)National Science Foundation (U.S.) (AST-1440343

Mapping our universe in 3D with MITEoR

Mapping our universe in 3D by imaging the redshifted 21 cm line from neutral hydrogen has the potential to overtake the cosmic microwave background as our most powerful cosmological probe, because it can map a much larger volume of our Universe, shedding new light on the epoch of reionization, inflation, dark matter, dark energy, and neutrino masses. We report on MITEoR, a pathfinder low-frequency radio interferometer whose goal is to test technologies that greatly reduce the cost of such 3D mapping for a given sensitivity. MITEoR accomplishes this by using massive baseline redundancy both to enable automated precision calibration and to cut the correlator cost scaling from N[superscript 2] to N log N, where N is the number of antennas. The success of MITEoR with its 64 dual-polarization elements bodes well for the more ambitious HERA project, which incorporates many identical or similar technologies using an order of magnitude more antennas, each with dramatically larger collecting area.National Science Foundation (U.S.) (Grant AST-0908848)National Science Foundation (U.S.) (Grant AST-1105835)MIT Kavli Instrumentation FundMassachusetts Institute of Technology. Undergraduate Research Opportunities Progra

Receiver development for BICEP Array, a next-generation CMB polarimeter at the South Pole

A detection of curl-type (B-mode) polarization of the primary CMB would be direct evidence for the inflationary paradigm of the origin of the Universe. The Bicep/Keck Array (BK) program targets the degree angular scales, where the power from primordial B-mode polarization is expected to peak, with ever-increasing sensitivity and has published the most stringent constraints on inflation to date. Bicep Array (BA) is the Stage-3 instrument of the BK program and will comprise four Bicep3-class receivers observing at 30/40, 95, 150 and 220/270 GHz with a combined 32,000+ detectors; such wide frequency coverage is necessary for control of the Galactic foregrounds, which also produce degree-scale B-mode signal. The 30/40 GHz receiver is designed to constrain the synchrotron foreground and has begun observing at the South Pole in early 2020. By the end of a 3-year observing campaign, the full Bicep Array instrument is projected to reach σr between 0.002 and 0.004, depending on foreground complexity and degree of removal of B-modes due to gravitational lensing (delensing). This paper presents an overview of the design, measured on-sky performance and calibration of the first BA receiver. We also give a preview of the added complexity in the time-domain multiplexed readout of the 7,776-detector 150 GHz receiver

Analysis of Temperature-to-Polarization Leakage in BICEP3 and Keck CMB Data from 2016 to 2018

The Bicep/Keck Array experiment is a series of small-aperture refracting telescopes observing degree-scale Cosmic Microwave Background polarization from the South Pole in search of a primordial B-mode signature. As a pair differencing experiment, an important systematic that must be controlled is the differential beam response between the co-located, orthogonally polarized detectors. We use high-fidelity, in-situ measurements of the beam response to estimate the temperature-to-polarization (T → P) leakage in our latest data including observations from 2016 through 2018. This includes three years of Bicep3 observing at 95 GHz, and multifrequency data from Keck Array. Here we present band-averaged far-field beam maps, differential beam mismatch, and residual beam power (after filtering out the leading difference modes via deprojection) for these receivers. We show preliminary results of "beam map simulations," which use these beam maps to observe a simulated temperature (no Q/U) sky to estimate T → P leakage in our real data