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In-sewer field-evaluation of an optical fibre-based condition monitoring system
A Fiber Bragg Grating (FBG) based monitoring system for continuous humidity and temperature measurement has been designed and evaluated experimentally in a sewer environment with high corrosion rates, humidity and the presence of gaseous hydrogen sulfide. The monitoring system has been designed specifically for field use, including packaging prepared for the harsh environment and the challenges of the operation. The system is battery powered and has hardware for controlling the interrogation equipment, power management, data logging and 4G connectivity. Results obtained show the long-term performance, over a 6-month period of non-stop monitoring of real-time data using the same probe. The data acquired was compared to the environmental data of temperature and precipitation for this period from the same location, which showed a good correlation between the expected and the measured data values. The data obtained point to the success of the optical fibre-based sensor system for monitoring in these harsh environments over long periods
Thermodynamics of a Colloidal Particle in a Time-Dependent Non-Harmonic Potential
We study the motion of an overdamped colloidal particle in a time-dependent
non-harmonic potential. We demonstrate the first law-like balance between
applied work, exchanged heat, and internal energy on the level of a single
trajectory. The observed distribution of applied work is distinctly
non-Gaussian in good agreement with numerical calculations. Both the Jarzynski
relation and a detailed fluctuation theorem are verified with good accuracy
A numerical approach to large deviations in continuous-time
We present an algorithm to evaluate the large deviation functions associated
to history-dependent observables. Instead of relying on a time discretisation
procedure to approximate the dynamics, we provide a direct continuous-time
algorithm, valuable for systems with multiple time scales, thus extending the
work of Giardin\`a, Kurchan and Peliti (PRL 96, 120603 (2006)).
The procedure is supplemented with a thermodynamic-integration scheme, which
improves its efficiency. We also show how the method can be used to probe large
deviation functions in systems with a dynamical phase transition -- revealed in
our context through the appearance of a non-analyticity in the large deviation
functions.Comment: Submitted to J. Stat. Mec
Stability in chromosome number and DNA content in synthetic tetraploids of Lolium multiflorum after two generations of selection.
Chromosome doubling of Italian ryegrass genotypes ( Lolium multiflorum Lam.) adapted to the brazilian edaphoclimatic conditions is an important strategy used by breeders and aims to obtain more vigorous genotypes with better forage quality and disease resistance. The effectiveness of chromosome doubling can be measured by genetic stability and fertility rates of plants over generations. However, a common problem in the polyploidization process is the regeneration of mixoploid plants that have impaired fertility and genetic stability. The objective of this study was to verify if progenies of recently tetraploidized plants remain stable regarding DNA content and chromosome number, over two generations. Progenies of L. multiflorum plants artificially tetraploidized with colchicine treatment were evaluated. Chromosome counting and estimates of the DNA content were used to evaluate the genetic stability. The percentage of tetraploid plants (4X) increased over generations (18%, 34% and 91% in cycle 0, 1 and 2, respectively). All progenies identified as tetraploid by flow citometry showed variation in chromosome number (mixoploidy), but produced viable seeds. Results showed that stabilization in chromosome number and DNA content in tetraploidized plant progenies requires time and that the success of this procedure depends on a continuous and accurate screening and selection
Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca.
Abstract Analyses carried out with fluorescence in situ hybridization (FISH) in C-metaphases of the Lolium-Festuca complex have shown the occurrence of spontaneous fragile sites (FSs) in 45S rDNA regions. FSs are expressed as gaps but they do not result in breaks or chromosomal fragments in these species. These gaps have high DNA condensation observed as thin chromatin fibers that connect the apparent segments of the fragile chromosome, allowing for genomic stability. Assessing the behavior of these regions in the cell cycle of Lolium and Festuca species may lead to a better understanding of the dynamics that preserve stability during cell division. Furthermore, it is interesting to track the dynamics of chromosomes bearing 45S rDNA sites in the cell cycle as well as to observe the expression of FSs with no effect of the mitotic block. We observed variation in both the number and size of 45S FISH signals from the S/G2 phases of interphase and from prophase to anaphase where gaps in 45S rDNA sites also were observed. The change in the degree of condensation of the 45S site begins in the S/G2 phase and appears to be related to the transcriptional demand. Taking into account that the number of 45S rDNA sites tends to be re-established when cells reach telophase, we suggest that the chromatin fiber goes back to the normal condensation level to the anaphase (after segregation), allowing for the approximation of chromosome segments and ensuring dynamics that favor the genomic stability of these species
Stochastic Physics, Complex Systems and Biology
In complex systems, the interplay between nonlinear and stochastic dynamics,
e.g., J. Monod's necessity and chance, gives rise to an evolutionary process in
Darwinian sense, in terms of discrete jumps among attractors, with punctuated
equilibrium, spontaneous random "mutations" and "adaptations". On an
evlutionary time scale it produces sustainable diversity among individuals in a
homogeneous population rather than convergence as usually predicted by a
deterministic dynamics. The emergent discrete states in such a system, i.e.,
attractors, have natural robustness against both internal and external
perturbations. Phenotypic states of a biological cell, a mesoscopic nonlinear
stochastic open biochemical system, could be understood through such a
perspective.Comment: 10 page
Elasticity model of a supercoiled DNA molecule
Within a simple elastic theory, we study the elongation versus force
characteristics of a supercoiled DNA molecule at thermal equilibrium in the
regime of small supercoiling. The partition function is mapped to the path
integral representation for a quantum charged particle in the field of a
magnetic monopole with unquantized charge.
We show that the theory is singular in the continuum limit and must be
regularised at an intermediate length scale. We find good agreement with
existing experimental data, and point out how to measure the twist rigidity
accurately.Comment: Latex, 4 pages. The figure contains new experimental data, giving a
new determination of the twist rigidit
Origin of chemically distinct discs in the Auriga cosmological simulations
The stellar disc of the Milky Way shows complex spatial and abundance structure that is central to understanding the key physical mechanisms responsible for shaping our Galaxy. In this study, we use six very high resolution cosmological zoom-in simulations of Milky Way-sized haloes to study the prevalence and formation of chemically distinct disc components. We find that our simulations develop a clearly bimodal distribution in the [α/Fe]–[Fe/H] plane. We find two main pathways to creating this dichotomy, which operate in different regions of the galaxies: (a) an early (z > 1) and intense high-[α/Fe] star formation phase in the inner region (R ≲ 5 kpc) induced by gas-rich mergers, followed by more quiescent low-[α/Fe] star formation; and (b) an early phase of high-[α/Fe] star formation in the outer disc followed by a shrinking of the gas disc owing to a temporarily lowered gas accretion rate, after which disc growth resumes. In process (b), a double-peaked star formation history around the time and radius of disc shrinking accentuates the dichotomy. If the early star formation phase is prolonged (rather than short and intense), chemical evolution proceeds as per process (a) in the inner region, but the dichotomy is less clear. In the outer region, the dichotomy is only evident if the first intense phase of star formation covers a large enough radial range before disc shrinking occurs; otherwise, the outer disc consists of only low-[α/Fe] sequence stars. We discuss the implication that both processes occurred in the Milky Way
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