The Standardized Fish Bioassay Procedure for Detecting and Culturing Actively Toxic Pfiesteria, Used by Two Reference Laboratories for Atlantic and Gulf Coast States

In the absence of purified standards of toxins from Pfiesteria species, appropriately conducted fish bioassays are the gold standard that must be used to detect toxic strains of Pfiesteria slop. from natural estuarine water or sediment samples and to culture actively toxic Pfiesteria. In this article, we describe the standardized steps of our fish bioassay as an abbreviated term for a procedure that includes two sets of trials with fish, following the Henle-Koch postulates modified for toxic rather than infectious agents. This procedure was developed in 1991, and has been refined over more than 12 years of experience in research with toxic Pfiesteria. The steps involve isolating toxic strains of Pfiesteria (or other potentially, as-yet-undetected, toxic Pfiesteria or Pfiesteria-like species) from fish-killing bioassays with natural samples; growing the clones with axenic algal prey; and retesting the isolates in a second set of fish bioassays. The specific environmental conditions used (e.g., temperature, salinity, light, other factors) must remain flexible, given the wide range of conditions from which natural estuarine samples are derived. We present a comparison of information provided for fish culture conditions, reported in international science journals in which such research is routinely published, and we provide information from more than 2,000 fish bioassays with toxic Pfiesteria, along with recommendations for suitable ranges and frequency of monitoring of environmental variables. We present data demonstrating that algal assays, unlike these standardized fish bioassays, should not be used to detect toxic strains of Pfiesteria spp. Finally, we recommend how quality control/assurance can be most rapidly advanced among laboratories engaged in studies that require research-quality isolates of toxic Pfiesteria spp

Sensors for ceramic components in advanced propulsion systems

This report includes: (1) a survey of the current methods for the measurement of surface temperature of ceramic materials suitable for use as hot section flowpath components in aircraft gas turbine engines; (2) analysis and selection of three sensing techniques with potential to extend surface temperature measurement capability beyond current limits; and (3) design, manufacture, and evaluation of the three selected techniques which include the following: platinum rhodium thin film thermocouple on alumina and mullite substrates; doped silicon carbide thin film thermocouple on silicon carbide, silicon nitride, and aluminum nitride substrates; and long and short wavelength radiation pyrometry on the substrates listed above plus yttria stabilized zirconia. Measurement of surface emittance of these materials at elevated temperature was included as part of this effort

Laboratory evaluation of the effect of nitric acid uptake on frost point hygrometer performance

Chilled mirror hygrometers (CMH) are widely used to measure water vapour in the troposphere and lower stratosphere from balloon-borne sondes. Systematic discrepancies among in situ water vapour instruments have been observed at low water vapour mixing ratios (<5 ppm) in the upper troposphere and lower stratosphere (UT/LS). Understanding the source of the measurement discrepancies is important for a more accurate and reliable determination of water vapour abundance in this region. We have conducted a laboratory study to investigate the potential interference of gas-phase nitric acid (HNO<sub>3</sub>) with the measurement of frost point temperature, and consequently the water vapour mixing ratio, determined by CMH under conditions representative of operation in the UT/LS. No detectable interference in the measured frost point temperature was found for HNO<sub>3</sub> mixing ratios of up to 4 ppb for exposure times up to 150 min. HNO<sub>3</sub> was observed to co-condense on the mirror frost, with the adsorbed mass increasing linearly with time at constant exposure levels. Over the duration of a typical balloon sonde ascent (90–120 min), the maximum accumulated HNO<sub>3</sub> amounts were comparable to monolayer coverage of the geometric mirror surface area, which corresponds to only a small fraction of the actual frost layer surface area. This small amount of co-condensed HNO<sub>3</sub> is consistent with the observed lack of HNO<sub>3</sub> interference in the frost point measurement because the CMH utilizes significant reductions (>10%) in surface reflectivity by the condensate to determine H<sub>2</sub>O

A search for Low Surface Brightness galaxies in the near-infrared I. Selection of the sample

A sample of about 3,800 Low Surface Brightness (LSB) galaxies was selected using the all-sky near-infrared (J, H and K_s-band) 2MASS survey. The selected objects have a mean central surface brightness within a 5 arcsec radius around their centre fainter than 18 mag/sq.arcsec in the K_s band, making them the lowest surface brightness galaxies detected by 2MASS. A description is given of the relevant properties of the 2MASS survey and the LSB galaxy selection procedure, as well as of basic photometric properties of the selected objects. The latter properties are compared to those of other samples of galaxies, of both LSBs and `classical' high surface brightness (HSB) objects, which were selected in the optical. The 2MASS LSBs have a (B_T_c)-(K_T) colour which is on average 0.9 mag bluer than that of HSBs from the NGC. The 2MASS sample does not appear to contain a significant population of red objects.Comment: accepted for publication in Astronomy and Astrophysics on 24/2/2003; 62 page

Fine material in grain

Fine material in grain: an overview / Richard Stroshine -- Factors that affect the costs of fines in the corn export market / Lowell D. Hill, Mack Leath -- Effects of fine material on mold growth in grain / David B. Sauer, Richard A. Meronuck, John Tuite -- Effects of fine material on insect infestation: a review / Paul W. Flinn, William H. McGaughey, Wendell E. Burkholder -- Reducing or controlling damage to grain from handling: a review / Charles R. Martin, George H. Foster -- Evaluating grain for potential production of fine material - breakage susceptibility testing / Steven R. Eckhoff -- Genotypic differences in breakage susceptibility of corn and soybeans -- M. R. Paulsen, L. L. Darrah, R. L. Stroshin

Limit theorems for von Mises statistics of a measure preserving transformation

For a measure preserving transformation $T$ of a probability space $(X,\mathcal F,\mu)$ we investigate almost sure and distributional convergence of random variables of the form $$x \to \frac{1}{C_n} \sum_{i_1<n,...,i_d<n} f(T^{i_1}x,...,T^{i_d}x),\, n=1,2,...,$$ where $f$ (called the \emph{kernel}) is a function from $X^d$ to $\R$ and $C_1, C_2,...$ are appropriate normalizing constants. We observe that the above random variables are well defined and belong to $L_r(\mu)$ provided that the kernel is chosen from the projective tensor product $$L_p(X_1,\mathcal F_1, \mu_1) \otimes_{\pi}...\otimes_{\pi} L_p(X_d,\mathcal F_d, \mu_d)\subset L_p(\mu^d)$$ with $p=d\,r,\, r\ \in [1, \infty).$ We establish a form of the individual ergodic theorem for such sequences. Next, we give a martingale approximation argument to derive a central limit theorem in the non-degenerate case (in the sense of the classical Hoeffding's decomposition). Furthermore, for $d=2$ and a wide class of canonical kernels $f$ we also show that the convergence holds in distribution towards a quadratic form $\sum_{m=1}^{\infty} \lambda_m\eta^2_m$ in independent standard Gaussian variables $\eta_1, \eta_2,...$. Our results on the distributional convergence use a $T$--\,invariant filtration as a prerequisite and are derived from uni- and multivariate martingale approximations

1,2-Dichlorohexafluoro-Cyclobutane (1,2-c-C4F6Cl2, R-316c) a Potent Ozone Depleting Substance and Greenhouse Gas: Atmospheric Loss Processes, Lifetimes, and Ozone Depletion and Global Warming Potentials for the (E) and (Z) stereoisomers

The atmospheric processing of (E)- and (Z)-1,2-dichlorohexafluorocyclobutane (1,2-c-C4F6Cl2, R-316c) was examined in this work as the ozone depleting (ODP) and global warming (GWP) potentials of this proposed replacement compound are presently unknown. The predominant atmospheric loss processes and infrared absorption spectra of the R-316c isomers were measured to provide a basis to evaluate their atmospheric lifetimes and, thus, ODPs and GWPs. UV absorption spectra were measured between 184.95 to 230 nm at temperatures between 214 and 296 K and a parametrization for use in atmospheric modeling is presented. The Cl atom quantum yield in the 193 nm photolysis of R- 316c was measured to be 1.90 +/- 0.27. Hexafluorocyclobutene (c-C4F6) was determined to be a photolysis co-product with molar yields of 0.7 and 1.0 (+/-10%) for (E)- and (Z)-R-316c, respectively. The 296 K total rate coefficient for the O(1D) + R-316c reaction, i.e., O(1D) loss, was measured to be (1.56 +/- 0.11) 10(exp 10)cu cm/ molecule/s and the reactive rate coefficient, i.e., R-316c loss, was measured to be (1.36 +/- 0.20) 10(exp 10)cu cm/molecule/s corresponding to a approx. 88% reactive yield. Rate coefficient upper-limits for the OH and O3 reaction with R-316c were determined to be <2.3 10(exp 17) and <2.0 10(exp 22)cu cm/molecule/s, respectively, at 296 K. The quoted uncertainty limits are 2(sigma) and include estimated systematic errors. Local and global annually averaged lifetimes for the (E)- and (Z)-R-316c isomers were calculated using a 2-D atmospheric model to be 74.6 +/- 3 and 114.1 +/-10 years, respectively, where the estimated uncertainties are due solely to the uncertainty in the UV absorption spectra. Stratospheric photolysis is the predominant atmospheric loss process for both isomers with the O(1D) reaction making a minor, approx. 2% for the (E) isomer and 7% for the (Z) isomer, contribution to the total atmospheric loss. Ozone depletion potentials for (E)- and (Z)-R-316c were calculated using the 2-D model to be 0.46 and 0.54, respectively. Infrared absorption spectra for (E)- and (Z)-R-316c were measured at 296 K and used to estimate their radiative efficiencies (REs) and GWPs; 100-year time-horizon GWPs of 4160 and 5400 were obtained for (E)- and (Z)-R-316c, respectively. Both isomers of R-316c are shown in this work to be long-lived ozone depleting substances and potent greenhouse gases

Generalized Huygens principle with pulsed-beam wavelets

Huygens' principle has a well-known problem with back-propagation due to the spherical nature of the secondary wavelets. We solve this by analytically continuing the surface of integration. If the surface is a sphere of radius $R$, this is done by complexifying $R$ to $R+ia$. The resulting complex sphere is shown to be a real bundle of disks with radius $a$ tangent to the sphere. Huygens' "secondary source points" are thus replaced by disks, and his spherical wavelets by well-focused pulsed beams propagating outward. This solves the back-propagation problem. The extended Huygens principle is a completeness relation for pulsed beams, giving a representation of a general radiation field as a superposition of such beams. Furthermore, it naturally yields a very efficient way to compute radiation fields because all pulsed beams missing a given observer can be ignored. Increasing $a$ sharpens the focus of the pulsed beams, which in turn raises the compression of the representation.Comment: 49 pages, 14 figure

Pulsed Feedback Defers Cellular Differentiation

Environmental signals induce diverse cellular differentiation programs. In certain systems, cells defer differentiation for extended time periods after the signal appears, proliferating through multiple rounds of cell division before committing to a new fate. How can cells set a deferral time much longer than the cell cycle? Here we study Bacillus subtilis cells that respond to sudden nutrient limitation with multiple rounds of growth and division before differentiating into spores. A well-characterized genetic circuit controls the concentration and phosphorylation of the master regulator Spo0A, which rises to a critical concentration to initiate sporulation. However, it remains unclear how this circuit enables cells to defer sporulation for multiple cell cycles. Using quantitative time-lapse fluorescence microscopy of Spo0A dynamics in individual cells, we observed pulses of Spo0A phosphorylation at a characteristic cell cycle phase. Pulse amplitudes grew systematically and cell-autonomously over multiple cell cycles leading up to sporulation. This pulse growth required a key positive feedback loop involving the sporulation kinases, without which the deferral of sporulation became ultrasensitive to kinase expression. Thus, deferral is controlled by a pulsed positive feedback loop in which kinase expression is activated by pulses of Spo0A phosphorylation. This pulsed positive feedback architecture provides a more robust mechanism for setting deferral times than constitutive kinase expression. Finally, using mathematical modeling, we show how pulsing and time delays together enable “polyphasic” positive feedback, in which different parts of a feedback loop are active at different times. Polyphasic feedback can enable more accurate tuning of long deferral times. Together, these results suggest that Bacillus subtilis uses a pulsed positive feedback loop to implement a “timer” that operates over timescales much longer than a cell cycle