Mathematical modelling of wave–structure interactions with application to wave energy conversion

Energy conversion from ocean waves is one of the core themes of the energy global challenge research for the sustainability of the planet. New trends of wave energytechnologies attempt to explore new paths and intend to bring new answers to the problem of the competitiveness of the produced energy for conventional devices. This thesis provides the reader with mathematical models of wave-structure interactions applied to novel concepts of wave energy converters: a flexible piezoelectric wave energy harvester and a floater blanket wave energy converter. In chapter 1 I present an overview of the field of wave energy, a brief history and descriptions of working principles and technologies of wave energy conversion along with a number of classication schemes. Classical systems as well as new trends in the form of flexible or deformable converters and hybrid systems are presented. [Continues.]</div

Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells

Background Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components. Modern vaccines are becoming increasingly complex, with the incorporation of multiple antigens. Approaches towards developing an HIV vaccine appear to confirm this, with a combination of candidate antigens. Among these, HIV-Nef is considered a promising target for vaccine development because immune responses directed against this viral protein could help to control the initial steps of viral infection and to reduce viral loads and spreading. Two isoforms of Nef protein can be found in cells: a full-length N-terminal myristoylated form (p27, 27 kDa) and a truncated form (p25, 25 kDa). Here we report the expression and purification of HIV Nef from transgenic tobacco. Results We designed constructs to direct the expression of p25 and p27 Nef to either the cytosol or the secretory pathway. We tested these constructs by transient expression in tobacco protoplasts. Cytosolic Nef polypeptides are correctly synthesised and are stable. The same is not true for Nef polypeptides targeted to the secretory pathway by virtue of a signal peptide. We therefore generated transgenic plants expressing cytosolic, full length or truncated Nef. Expression levels were variable, but in some lines they averaged 0.7% of total soluble proteins. Hexahistidine-tagged Nef was easily purified from transgenic tissue in a one-step procedure. Conclusion We have shown that transient expression can help to rapidly determine the best cellular compartment for accumulation of a recombinant protein. We have successfully expressed HIV Nef polypeptides in the cytosol of transgenic tobacco plants. The proteins can easily be purified from transgenic tissue

Human adenocarcinoma cell line sensitivity to essential oil phytocomplexes from pistacia species: A multivariate approach

Principal component analysis (PCA) multivariate analysis was applied to study the cytotoxic activity of essential oils from various species of the Pistacia genus on human tumor cell lines. In particular, the cytotoxic activity of essential oils obtained from P. lentiscus, P. lentiscus var. chia (mastic gum), P. terebinthus, P. vera, and P. integerrima, was screened on three human adenocarcinoma cell lines: MCF-7 (breast), 2008 (ovarian), and LoVo (colon). The results indicate that all the Pistacia phytocomplexes, with the exception of mastic gum oil, induce cytotoxic effects on one or more of the three cell lines. PCA highlighted the presence of different cooperating clusters of bioactive molecules. Cluster variability among species, and even within the same species, could explain some of the differences seen among samples suggesting the presence of both common and species-specific mechanisms. Single molecules from one of the most significant clusters were tested, but only bornyl-acetate presented cytotoxic activity, although at much higher concentrations (IC50 = 138.5 \ub5g/mL) than those present in the essential oils, indicating that understanding of the full biological effect requires a holistic vision of the phytocomplexes with all its constituents

Pharmacogenomic characterization in bipolar spectrum disorders

The holistic approach of personalized medicine, merging clinical and molecular characteristics to tailor the diagnostic and therapeutic path to each individual, is steadily spreading in clinical practice. Psychiatric disorders represent one of the most difficult diagnostic challenges, given their frequent mixed nature and intrinsic variability, as in bipolar disorders and depression. Patients misdiagnosed as depressed are often initially prescribed serotonergic antidepressants, a treatment that can exacerbate a previously unrecognized bipolar condition. Thanks to the use of the patient\u2019s genomic profile, it is possible to recognize such risk and at the same time characterize specific genetic assets specifically associated with bipolar spectrum disorder, as well as with the individual response to the various therapeutic options. This provides the basis for molecular diagnosis and the definition of pharmacogenomic profiles, thus guiding therapeutic choices and allowing a safer and more effective use of psychotropic drugs. Here, we report the pharmacogenomics state of the art in bipolar disorders and suggest an algorithm for therapeutic regimen choice

Essential oil phytocomplex activity, a review with a focus on multivariate analysis for a network pharmacology-informed phytogenomic approach

Thanks to omic disciplines and a systems biology approach, the study of essential oils and phytocomplexes has been lately rolling on a faster track. While metabolomic fingerprinting can provide an effective strategy to characterize essential oil contents, network pharmacology is revealing itself as an adequate, holistic platform to study the collective effects of herbal products and their multi-component and multi-target mediated mechanisms. Multivariate analysis can be applied to analyze the effects of essential oils, possibly overcoming the reductionist limits of bioactivity-guided fractionation and purification of single components. Thanks to the fast evolution of bioinformatics and database availability, disease-target networks relevant to a growing number of phytocomplexes are being developed. With the same potential actionability of pharmacogenomic data, phytogenomics could be performed based on relevant disease-target networks to inform and personalize phytocomplex therapeutic application

Spotlight on measles 2010: Increased measles transmission in Ferrara, Italy, despite high vaccination coverage, March to May 2010

We report an increase in the incidence of measles in a population with consistently high and improving immunisation coverage in Ferrara province, northern Italy. During the first six months of 2010, 19 cases were confirmed, 10 of which were hospitalised. General practitioners, paediatricians and local healthcare authorities were alerted about the outbreak and asked to notify all suspected cases. We need to further increase immunisation coverage and to maintain and implement the monitoring system

Neuromuscular Taping Application in Counter Movement Jump: Biomechanical Insight in a Group of Healthy Basketball Players.

Kinesiologic elastic tape is widely used for both clinical and sport applications although its efficacy in enhancing agonistic performance is still controversial. Aim of the study was to verify in a group of healthy basketball players whether a neuromuscular taping application (NMT) on ankle and knee joints could affect the kinematic and the kinetic parameters of the jump, either by enhancing or inhibiting the functional performance. Fourteen healthy male basketball players without any ongoing pathologies at upper limbs, lower limbs and trunk volunteered in the study. They randomly performed 2 sets of 5 counter movement jumps (CMJ) with and without application of Kinesiologic tape. The best 3 jumps of each set were considered for the analysis. The Kinematics parameters analyzed were: knees maximal flexion and ankles maximal dorsiflexion during the push off phase, jump height and take off velocity. Vertical ground reaction force and maximal power expressed in the push off phase of the jump were also investigated. The NMT application in both knees and ankles showed no statistically significant differences in the kinematic and kinetic parameters and did not interfere with the CMJ performance. Bilateral NMT application in the group of healthy male basketball players did not change kinematics and kinetics jump parameters, thus suggesting that its routine use should have no negative effect on functional performance. Similarly, the combined application of the tape on both knees and ankles did not affect in either way jump performance

Power extraction from floating elastic plates

We present a novel mathematical model to investigate the extraction of wave power by flexible elastic floaters. The model is based on the method of dry modes, coupled with a matched eigenfunction expansion. Our model results compare satisfactorily with preliminary data obtained from a demonstrator device, developed at the University of Groningen. We show that the role of elasticity is to increase the number of resonant frequencies with respect to a rigid body, which has a positive effect on wave power output. The mathematical model is then extended to irregular incident waves, described by a JONSWAP spectrum. Our results show that the peak capture factors decrease in irregular waves, as compared to the monochromatic case. However, the system becomes more efficient at non-resonant frequencies. This work highlights the need to scale-up experimental investigations on flexible wave energy converters, which are still a small minority, compared to those on rigid converters

Power extraction from floating elastic plates

We present a novel mathematical model to investigate the extraction of wave power by flexible elastic floaters. The model is based on the method of dry modes, coupled with a matched eigenfunction expansion. Our model results compare satisfactorily with preliminary data obtained from a demonstrator device, developed at the University of Groningen. We show that the role of elasticity is to increase the number of resonant frequencies with respect to a rigid body, which has a positive effect on wave power output. The mathematical model is then extended to irregular incident waves, described by a JONSWAP spectrum. Our results show that the peak capture factors decrease in irregular waves, as compared to the monochromatic case. However, the system becomes more efficient at non-resonant frequencies. This work highlights the need to scale-up experimental investigations on flexible wave energy converters, which are still a small minority, compared to those on rigid converters