Endotoxin receptor CD14 in PiZ α-1-antitrypsin deficiency individuals

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A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants

The World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost surveillance and easy to implement approach to evaluate antibiotic resistance in wastewater treatment plants (WWTPs) by targeting cefotaxime-resistant (CTX-R) coliforms as indicators. The rationale for this approach was: i) coliform quantification methods are internationally accepted as indicators of fecal contamination in recreational waters and are therefore routinely applied in analytical labs; ii) CTX-R coliforms are clinically relevant, associated with extended-spectrum β-lactamases (ESBLs), and are rare in pristine environments. We analyzed 57 WWTPs in 22 countries across Europe, Asia, Africa, Australia, and North America. CTX-R coliforms were ubiquitous in raw sewage and their relative abundance varied significantly (<0.1% to 38.3%), being positively correlated (p < 0.001) with regional atmospheric temperatures. Although most WWTPs removed large proportions of CTX-R coliforms, loads over 103 colony-forming units per mL were occasionally observed in final effluents. We demonstrate that CTX-R coliform monitoring is a feasible and affordable approach to assess wastewater antibiotic resistance status

Finding the Needles in the Metagenome Haystack

In the collective genomes (the metagenome) of the microorganisms inhabiting the Earth’s diverse environments is written the history of life on this planet. New molecular tools developed and used for the past 15 years by microbial ecologists are facilitating the extraction, cloning, screening, and sequencing of these genomes. This approach allows microbial ecologists to access and study the full range of microbial diversity, regardless of our ability to culture organisms, and provides an unprecedented access to the breadth of natural products that these genomes encode. However, there is no way that the mere collection of sequences, no matter how expansive, can provide full coverage of the complex world of microbial metagenomes within the foreseeable future. Furthermore, although it is possible to fish out highly informative and useful genes from the sea of gene diversity in the environment, this can be a highly tedious and inefficient procedure. Microbial ecologists must be clever in their pursuit of ecologically relevant, valuable, and niche-defining genomic information within the vast haystack of microbial diversity. In this report, we seek to describe advances and prospects that will help microbial ecologists glean more knowledge from investigations into metagenomes. These include technological advances in sequencing and cloning methodologies, as well as improvements in annotation and comparative sequence analysis. More significant, however, will be ways to focus in on various subsets of the metagenome that may be of particular relevance, either by limiting the target community under study or improving the focus or speed of screening procedures. Lastly, given the cost and infrastructure necessary for large metagenome projects, and the almost inexhaustible amount of data they can produce, trends toward broader use of metagenome data across the research community coupled with the needed investment in bioinformatics infrastructure devoted to metagenomics will no doubt further increase the value of metagenomic studies in various environments

Crustal Azimuthal Anisotropy Beneath the Central North China Craton Revealed by Receiver Functions

To characterize crustal anisotropy beneath the central North China Craton (CNCC), we apply a recently developed deconvolution approach to effectively remove near-surface reverberations in the receiver functions recorded at 200 broadband seismic stations and subsequently determine the fast orientation and the magnitude of crustal azimuthal anisotropy by fitting the sinusoidal moveout of the P to S converted phases from the Moho and intracrustal discontinuities. The magnitude of crustal anisotropy is found to range from 0.06 s to 0.54Â s, with an average of 0.25 ± 0.08Â s. Fault-parallel anisotropy in the seismically active Zhangjiakou-Penglai Fault Zone is significant and could be related to fluid-filled fractures. Historical strong earthquakes mainly occurred in the fault zone segments with significant crustal anisotropy, suggesting that the measured crustal anisotropy is closely related to the degree of crustal deformation. The observed spatial distribution of crustal anisotropy suggests that the northwestern terminus of the fault zone probably ends at about 114°E. Also observed is a sharp contrast in the fast orientations between the western and eastern Yanshan Uplifts separated by the North-South Gravity Lineament. The NW-SE trending anisotropy in the western Yanshan Uplift is attributable to fossil crustal anisotropy due to lithospheric extension of the CNCC, while extensional fluid-saturated microcracks induced by regional compressive stress are responsible for the observed ENE-WSW trending anisotropy in the eastern Yanshan Uplift. Comparison of crustal anisotropy measurements and previously determined upper mantle anisotropy implies that the degree of crust-mantle coupling in the CNCC varies spatially

'Another World is Possible': A Study of Participants at Australian Alter-Globalisation Social Forums

The past decade has seen the emergence of a mass 'alter-globalisation' movement in many regions of the world. One element in this movement has been the World Social Forum and its continental, regional, national and local spin-offs. In the first half of this article, I provide a critical analysis of the social forum experience, particularly the World Social Forum, and outline both those aspects of the experience that are commonly agreed as successes as well as those that are frequently held to be their failings or limitations. In the second half of the article, I report on a survey of the participants at two Australian social forums in 2004 which details their backgrounds, motivations, attitudes, experience, and ambitions. Comparison is made with their closest parallels - the activists from the new social movements of the 1970s and 1980s previously examined by Offe, Touraine, Melucci and others