Effect of occupational exposure to cytostatics and nucleotide excision repair polymorphism on chromosomal aberrations frequency

Authors evaluated the incidence of total chromosomal aberrations (CA) and their types – chromatid-type (CTA) and chromosome-type (CSA) in peripheral blood lymphocytes from 72 oncologic unit's workers occupationally exposed to cytostatics in relationship to polymorphisms of DNA repair genes XPD, XPG and XPC. The cytogenetic analysis was used for determination of chromosomal aberrations frequency and PCR-RFLP method for polymorphisms of genes. Statistically higher frequency of total CA was detected in exposed group as compared to control (1.90±1.34% vs. 1.26±0.93%; Mann-Whitney U-test, p=0.001). There was not detected any difference between CTA and CSA (0.92±1.04% vs. 0.98±1.17%). Similarly, in genes XPD exon 23 and XPC exon 15 wasn't detected any difference neither in total chromosomal aberrations nor in CTA and CSA types. Statistically significant decrease of total chromosomal aberrations and CTA-type with presence of variant allele C was detected in gene XPG exon 15. Authors pointed out the importance of individual susceptibility factors in evaluation of effects of genotoxic agents, in that event, when the concentration does not meet the occupational exposure limit

Gaia16apd – a link between fast and slowly declining type I superluminous supernovae

We present ultraviolet (UV), optical and infrared photometry and optical spectroscopy of the type Ic superluminous supernova (SLSN) Gaia16apd (=SN 2016eay), covering its evolution from 26 d before the g-band peak to 234.1 d after the peak. Gaia16apd was followed as a part of the NOT Unbiased Transient Survey (NUTS). It is one of the closest SLSNe known (z = 0.102 ± 0.001), with detailed optical and UV observations covering the peak. Gaia16apd is a spectroscopically typical type Ic SLSN, exhibiting the characteristic blue early spectra with O ii absorption, and reaches a peak Mg = −21.8 ± 0.1 mag. However, photometrically it exhibits an evolution intermediate between the fast and slowly declining type Ic SLSNe, with an early evolution closer to the fast-declining events. Together with LSQ12dlf, another SLSN with similar properties, it demonstrates a possible continuum between fast and slowly declining events. It is unusually UV-bright even for an SLSN, reaching a non-K-corrected Muvm2 ≃ −23.3 mag, the only other type Ic SLSN with similar UV brightness being SN 2010gx. Assuming that Gaia16apd was powered by magnetar spin-down, we derive a period of P = 1.9 ± 0.2 ms and a magnetic field of B = 1.9 ± 0.2 × 10$^{14}$ G for the magnetar. The estimated ejecta mass is between 8 and 16 M⊙, and the kinetic energy between 1.3 and 2.5 × 10$^{52}$ erg, depending on opacity and assuming that the entire ejecta is swept up into a thin shell. Despite the early photometric differences, the spectra at late times are similar to slowly declining type Ic SLSNe, implying that the two subclasses originate from similar progenitors.TK acknowledges financial support by the Emil Aaltonen Foundation. NB was supported by the GROWTH project funded by the National Science Foundation under Grant No. 1545949. JH acknowledges support by the Finnish Cultural Foundation. MF acknowledges the support of a Royal Society – Science Foundation Ireland University Research Fellowship. NER acknowledges financial support by the 1994 PRIN-INAF 2014 (project ‘Transient Universe: unveiling new types of stellar explosions with PESSTO’) and by MIUR PRIN 2010-2011, ‘The dark Universe and the cosmic evolution of baryons: from current surveys to Euclid’. EYH acknowledges the support provided by the National Science Foundation under Grant No. AST-1008343 and AST-1613472. MDS is funded by generous support provided by the Danish Agency for Science and Technology and Innovation realized through a Sapere Aude Level 2 grant and a grant from the Villum Foundation. This work was supported by the MINECO (Spanish Ministry of Economy) – FEDER through grants ESP2016-80079-C2-1-R and ESP2014-55996-C2-1-R and MDM-2014-0369 of ICCUB (Unidad de Excelencia ‘María de Maeztu’). NUTS is funded in part by the IDA (Instrument Centre for Danish Astronomy). ŁW was supported by Polish National Science Centre Grant No. OPUS 2015/17/B/ST9/03167

Gaia16apd-a link between fast and slowly declining type I superluminous supernovae

We present ultraviolet (UV), optical and infrared photometry and optical spectroscopy of the type Ic superluminous supernova (SLSN) Gaia16apd (= SN 2016eay), covering its evolution from 26 d before the g-band peak to 234.1 d after the peak. Gaia16apd was followed as a part of the NOT Unbiased Transient Survey (NUTS). It is one of the closest SLSNe known (z = 0.102 +/- 0.001), with detailed optical and UV observations covering the peak. Gaia16apd is a spectroscopically typical type Ic SLSN, exhibiting the characteristic blue early spectra with O II absorption, and reaches a peak M-g = -21.8 +/- 0.1 mag. However, photometrically it exhibits an evolution intermediate between the fast and slowly declining type Ic SLSNe, with an early evolution closer to the fast-declining events. Together with LSQ12dlf, another SLSN with similar properties, it demonstrates a possible continuum between fast and slowly declining events. It is unusually UV-bright even for an SLSN, reaching a non-K-corrected M-uvm2 similar or equal to -23.3 mag, the only other type Ic SLSN with similar UV brightness being SN 2010gx. Assuming that Gaia16apd was powered by magnetar spin-down, we derive a period of P = 1.9 +/- 0.2 ms and a magnetic field of B = 1.9 +/- 0.2 x 10(14) G for the magnetar. The estimated ejecta mass is between 8 and 16 M circle dot, and the kinetic energy between 1.3 and 2.5 x 10(52) erg, depending on opacity and assuming that the entire ejecta is swept up into a thin shell. Despite the early photometric differences, the spectra at late times are similar to slowly declining type Ic SLSNe, implying that the two subclasses originate from similar progenitors

SN 2022joj: A Peculiar Type Ia Supernova Possibly Driven by an Asymmetric Helium-shell Double Detonation

We present observations of SN 2022joj, a peculiar Type Ia supernova discovered by the Zwicky Transient Facility. SN 2022joj exhibits an unusually red g ZTF − r ZTF color at early times and a rapid blueward evolution afterward. Around maximum brightness, SN 2022joj shows a high luminosity ( M g ZTF , max ≃ − 19.7 mag), a blue broadband color (g ZTF − r ZTF ≃ −0.2 mag), and shallow Si ii absorption lines, consistent with those of overluminous, SN 1991T-like events. The maximum-light spectrum also shows prominent absorption around 4200 Å, which resembles the Ti ii features in subluminous, SN 1991bg-like events. Despite the blue optical-band colors, SN 2022joj exhibits extremely red ultraviolet minus optical colors at maximum luminosity (u − v ≃ 0.6 mag and uvw1 − v ≃ 2.5 mag), suggesting a suppression of flux at ∼2500-4000 Å. Strong C ii lines are also detected at peak. We show that these unusual spectroscopic properties are broadly consistent with the helium-shell double detonation of a sub-Chandrasekhar mass (M ≃ 1 M ⊙) carbon/oxygen white dwarf from a relatively massive helium shell (M s ≃ 0.04-0.1 M ⊙), if observed along a line of sight roughly opposite to where the shell initially detonates. None of the existing models could quantitatively explain all the peculiarities observed in SN 2022joj. The low flux ratio of [Ni ii] λ7378 to [Fe ii] λ7155 emission in the late-time nebular spectra indicates a low yield of stable Ni isotopes, favoring a sub-Chandrasekhar mass progenitor. The significant blueshift measured in the [Fe ii] λ7155 line is also consistent with an asymmetric chemical distribution in the ejecta, as is predicted in double-detonation models

Antineoplastic Drugs as a Potential Risk Factor in Occupational Settings: Mechanisms of Action at the Cell Level, Genotoxic Effects, and Their Detection Using Different Biomarkers

U članku je prikazana osnovna podjela antineoplastičnih lijekova prema mehanizmima djelovanja na razini stanice. Objašnjeni su mehanizmi genotoksičnosti najvažnijih vrsta lijekova koji se primjenjuju u okviru uobičajenih protokola za liječenje zloćudnih novotvorina. Navedena je važeća klasifi kacija antineoplastika prema kancerogenom potencijalu, podaci o mutagenom potencijalu te je prikazana njihova podjela u skladu s anatomsko-terapijsko-kemijskim sustavom klasifi kacije. Sustavno su prikazani najvažniji rezultati svjetskih i hrvatskih istraživanja na populacijama radnika izloženih antineoplasticima, provedenih u razdoblju 1980.-2009. s pomoću četiri najčešće primjenjivane metode: analize izmjena sestrinskih kromatida, analize kromosomskih aberacija, mikronukleus-testa i komet-testa. Objašnjena su osnovna načela navedenih metoda te raspravljene njihove prednosti i nedostaci. Biološki pokazatelji daju važne podatke o individualnoj osjetljivosti profesionalno izloženih ispitanika koji mogu poslužiti unaprjeđenju postojećih uvjeta rada i upravljanju rizicima pri izloženosti genotoksičnim agensima. Na osnovi prednosti i nedostataka citogenetičkih metoda zaključeno je da je mikronukleus-test, koji podjednako uspješno dokazuje klastogene i aneugene učinke, jedna od najboljih metoda dostupnih za otkrivanje štetnih djelovanja antineoplastičnih lijekova koji su u aktivnoj primjeni.This article brings an overview of the mechanisms of action of antineoplastic drugs used in the clinical setting. It also describes the genotoxic potentials of the most important classes of antineoplastic drugs involved in standard chemotherapy protocols. Classifi cation of antineoplastic drugs according to the IARC monographs on the evaluation of carcinogenic risks to humans is accompanied by data on their mutagenicity and the most recent updates in the Anatomical Therapeutic Chemical (ATC) Classifi cation System. We report the main fi ndings of biomonitoring studies that were conducted in exposed healthcare workers all over the world between 1980 and 2009 using four biomarkers: sister chromatid exchanges, chromosome aberrations, micronuclei. and the comet assay. The methods are briefl y explained and their advantages and disadvantages discussed. Biomarkers provide important information on individual genome sensitivity, which eventually might help to improve current working practices and to manage the risks related with exposure to genotoxic agents. Taking into consideration all known advantages and drawbacks of the existing cytogenetic methods, the micronucleus assay, which is able to detect both clastogenic and aneugenic action, is the most suitable biomarker for assessing harmful effects of antineoplastic drugs currently used in health care

Morphology and tensile properties of multigraft copolymers with regularly spaced tri-, tetra-, and hexafunctional junction points

The effect of chain architecture on the morphological and tensile properties of series of multigraft copolymers, with regularly spaced tri-, tetra-, and hexafunctional junction points, was investigated using transmission electron microscopy (TEM), small-angle X-ray scattering (SAXS), and tensile testing. The materials were synthesized by coupling difunctional polyisoprene (PI) spacers and living polystyrene (PS) branches, made by anionic polymerization, with chlorosilanes of different functionalities. Since the coupling process is a step-growth polymerization, yielding polydisperse products, fractionation was utilized to separate each material into three fractions (high, middle, and low molecular weight), each of low polydispersity. All three fractions have the same chain architecture on a per junction point basis but differ in the number of junction point units per molecule. By applying the constituting block copolymer concept, the physical behavior of these molecules was compared with current theories. It was found that morphological behavior of these graft copolymers can be predicted using theoretical approaches and is independent on the number of junction points. The number of the junction points, however, greatly influences the long-range order of microphase separation. Additionally, two new parameters for adjusting mechanical properties of multigraft copolymers were found in this investigation: (1) functionality of the graft copolymerstri-, tetra-, or hexafunctionals and (2) number of junction points per molecule. An increase in functionality causes a change in morphology, resulting in a high level of tensile strength for tetrafunctional (cylindrical) and hexafunctional (lamellae) multigraft copolymers, leading to about the twice the strength of the spherical trifunctional multigrafts of similar overall composition. Tetrafunctional multigraft copolymers show a surprisingly high strain at break, far exceeding that of commercial block copolymer thermoplastic elastomers (TPEs). Strain at break and tensile strength increase linearly with the number of junction points per molecule. Hysteresis experiments at about 300-900% deformation demonstrate that multifunctional multigraft copolymers have improved high elasticity as compared to commercial TPEs like Kraton or Styroflex

Gaia16apd - A link between fast and slowly declining type I superluminous supernovae

We present ultraviolet (UV), optical and infrared photometry and optical spectroscopy of the type Ic superluminous supernova (SLSN) Gaia16apd (=SN 2016eay), covering its evolution from 26 d before the g-band peak to 234.1 d after the peak. Gaia16apd was followed as a part of the NOT Unbiased Transient Survey (NUTS). It is one of the closest SLSNe known (z = 0.102 ± 0.001), with detailed optical and UV observations covering the peak. Gaia16apd is a spectroscopically typical type Ic SLSN, exhibiting the characteristic blue early spectra with O II absorption, and reaches a peak Mg = -21.8 ± 0.1 mag. However, photometrically it exhibits an evolution intermediate between the fast and slowly declining type Ic SLSNe, with an early evolution closer to the fast-declining events. Together with LSQ12dlf, another SLSN with similar properties, it demonstrates a possible continuum between fast and slowly declining events. It is unusually UV-bright even for an SLSN, reaching a non-K-corrected Muvm2 ? -23.3 mag, the only other type Ic SLSN with similar UV brightness being SN 2010gx. Assuming that Gaia16apd was powered by magnetar spin-down, we derive a period of P = 1.9 ± 0.2 ms and a magnetic field of B = 1.9 ± 0.2 × 1014 G for the magnetar. The estimated ejecta mass is between 8 and 16 M?, and the kinetic energy between 1.3 and 2.5 × 1052 erg, depending on opacity and assuming that the entire ejecta is swept up into a thin shell. Despite the early photometric differences, the spectra at late times are similar to slowly declining type Ic SLSNe, implying that the two subclasses originate from similar progenitors. © 2017 The Authors.Villum Fonden Federación Española de Enfermedades Raras, FEDER: ESP2016-80079-C2-1-R, MDM-2014-0369, ESP2014-55996-C2-1-R AST-1008343, PRIN 2010-2011, AST-1613472 National Science Foundation, NSF: 1545949 Suomen Kulttuurirahasto 1994 PRIN-INAF 2014 Instituto de Ciencias del Cosmos, Universitat de Barcelona, ICCUB Ministry of Economy Infocomm Development Authority of Singapore, IDA: Danish Astronomy Emil Aaltosen Säätiö International Parkinson and Movement Disorder Society, MDS National Centre for Atmospheric Science, NCAS: OPUS 2015/17/B/ST9/03167 Københavns Universitet, KU Ministerio de Economía y Competitividad, MINECOWe thank the anonymous referee for comments that helped improve this paper considerably. We thank Subo Dong, Cosimo Inserra, Christa Gall, Peter Brown and Andrea Pastorello for their suggestions. We acknowledge ESA Gaia, DPAC and the Photometric Science Alerts Team (http://gsaweb.ast.cam.ac.uk/alerts). The pt5m and TJO preliminary photometric calibrations were obtained using the Cambridge Photometric Calibration Server (CPCS), designed and maintained by Sergey Koposov and Lukasz Wyrzykowski. Based on observations made with the Nordic Optical Telescope, operated by the Nordic Optical Telescope Scientific Association, and with the Gran Telescopio Canarias (GTC), at the Observatorio del Roque de los Muchachos, La Palma, Spain, of the Instituto de Astrofisica de Canarias. The data presented here were obtained in part with ALFOSC,which is provided by the Instituto deAstrofisica de Andalucia (IAA) under a joint agreement with the University of Copenhagen and NOTSA. We also thank support astronomers working in Telescopi Joan Or? at Montsec Observatory (OAdM) for their help to run the needed sequences on time for our Gaia alert programme. TK acknowledges financial support by the Emil Aaltonen Foundation. NB was supported by the GROWTH project funded by the National Science Foundation under Grant No. 1545949. JH acknowledges support by the Finnish Cultural Foundation. MF acknowledges the support of a Royal Society-Science Foundation Ireland University Research Fellowship. NER acknowledges financial support by the 1994 PRIN-INAF 2014 (project 'Transient Universe: unveiling new types of stellar explosions with PESSTO') and by MIUR PRIN 2010-2011, 'The darkUniverse and the cosmic evolution of baryons: from current surveys to Euclid'. EYH acknowledges the support provided by the National Science Foundation under Grant No. AST-1008343 and AST-1613472. MDS is funded by generous support provided by the DanishAgency for Science and Technology and Innovation realized through a Sapere Aude Level 2 grant and a grant from the Villum Foundation. This work was supported by the MINECO (Spanish Ministry of Economy)-FEDER through grants ESP2016-80079-C2-1-R and ESP2014-55996-C2-1-R and MDM-2014-0369 of ICCUB (Unidad de Excelencia 'Mar?a de Maeztu'). NUTS is funded in part by the IDA (Instrument Centre for Danish Astronomy). LW was supported by Polish National Science Centre Grant No. OPUS 2015/17/B/ST9/03167. -

Gaia16apd – a link between fast and slowly declining type I superluminous supernovae

We present ultraviolet (UV), optical and infrared photometry and optical spectroscopy of the type Ic superluminous supernova (SLSN) Gaia16apd (=SN 2016eay), covering its evolution from 26 d before the g-band peak to 234.1 d after the peak. Gaia16apd was followed as a part of the NOT Unbiased Transient Survey (NUTS). It is one of the closest SLSNe known (z = 0.102 ± 0.001), with detailed optical and UV observations covering the peak. Gaia16apd is a spectroscopically typical type Ic SLSN, exhibiting the characteristic blue early spectra with O II absorption, and reaches a peak Mg = −21.8 ± 0.1 mag. However, photometrically it exhibits an evolution intermediate between the fast and slowly declining type Ic SLSNe, with an early evolution closer to the fast-declining events. Together with LSQ12dlf, another SLSN with similar properties, it demonstrates a possible continuum between fast and slowly declining events. It is unusually UV-bright even for an SLSN, reaching a non-K-corrected Muvm2 −23.3 mag, the only other type Ic SLSN with similar UV brightness being SN 2010gx. Assuming that Gaia16apd was powered by magnetar spin-down, we derive a period of P = 1.9 ± 0.2 ms and a magnetic field of B = 1.9 ± 0.2 × 1014 G for the magnetar. The estimated ejecta mass is between 8 and 16 M, and the kinetic energy between 1.3 and 2.5 × 1052 erg, depending on opacity and assuming that the entire ejecta is swept up into a thin shell. Despite the early photometric differences, the spectra at late times are similar to slowly declining type Ic SLSNe, implying that the two subclasses originate from similar progenitors