Detection of resistance to Citrus tristeza virus (CTV) with molecular marker in some citrus hybrids.

TEZ12336Tez (Yüksek Lisans) -- Çukurova Üniversitesi, Adana, 2017.Kaynakça (s. 75-89) var.XV, 116 s. :_res. (gnl. rnk.), tablo ;_29 cm.Türkiye coğrafik konumundan dolayı gen merkezi olmayan birçok bitkinin ekolojik isteklerini karşılamaktadır. Turunçgiller, Ülkemizin sahip olduğu ekolojik koşullarda rahatlıkla yetişebilen, besleyici özelliğe sahip ve geniş alanlarda üretimi yapılan bir bitki grubudur. Ancak, diğer bitkisel ürünlerimizde olduğu gibi yetiştiriciliği sınırlayan biyotik ve abiyotik etmenler mevcuttur. Dünyada salgın yapan tristeza virüsü, turunçgil bahçelerini zarara uğratmış, üretim miktarını azaltıp kaliteyi düşürmüştür. Bu virüs Türkiye’ye enfekteli aşı materyalleri ile giriş yapmıştır ancak etkili vektörü ülkemizde bulunmamaktadır. Ülkemiz genelinde, özellikle üretimin yoğun bir şekilde yapıldığı Akdeniz bölgesinde kullanılan turunç anacı bu virüse karşı duyarlıdır. Bu çalışmada, turunca alternatif olabilecek Tristeza’ya dayanıklı anaç geliştirmek amacı ile dayanıklı ebeveynler kullanılarak oluşturulan üç farklı melezleme kombinasyonlarından elde edilen melez bitkiler, haritalama sonucu dayanıklılık geninin varlığını gösteren 35 RAPD markörü ile taranmıştır. Bu çalışma ile Turunçgil anaç ıslahında Tristeza’ya dayanıklılık için erken seleksiyon markörlerinin farklı populasyonlarda kullanılabilirliği ortaya koyulmuştur.Turkey meets ecological desires of many plants that are not gene center, because of geographical location. Citrus that have nutritive properties and produced in large areas is a group of plants that can grow easily on the ecological conditions in our country. However, citrus cultivation is limited by biotic and abiotic factors like other herbal products. Tristeza virus which affects the citrus orchards around the world, has damaged citrus orchards, reducing the amount of production and decreasing the quality. This virus has been entered with infected graft materials in Turkey, but effective vector is not available in our country. Sour orange that used as citrus rootstock especially Mediterranean Region where production is performed intensively in our country, is susceptible to tristeza virus. In this study, hybrid plants that obtained from three different hybridization combinations formed by using Tristeza-resistant parent, were screened with 35 RAPD markers indicating the presence of the resistance gene. This study demonstrated utility of early selection markers for citrus rootstock breeding for resistance to tristeza virus in different population.Bu çalışma Ç.Ü. Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir. Proje No: FYL-2014-2794

Disruptive Mood Dysregulation Disorder in A Primary School Sample

Objective: This study aimed to determine frequency of DMDD in a community-based sample and to identify comorbid diagnoses in children with DMDD. Methods: The Child Behavior Checklist was given to 650 children attending the primary school and 453 children constituted the sample of the study. 83 children with a total score of 180 in CBCL attention, anxious/depression and aggression subscale scores were evaluated as moderate risk group (MRG) and 14 children with a total score of 210 and higher were considered as high-risk group (HRG) in terms of DMDD. 30 (6.62%of the study sample) from MRG and HRG agreed to participate in the study. DSM-V based psychiatric interview (PI) was done by a child and adolescent psychiatrist. Results: Eight of 30 children who underwent PI were within the high-risk group (HRG), and 4 of these children were diagnosed with DMMD (0.88% of the total sample). There was at least one comorbid diagnosis in all four children diagnosed with DMMD. Conclusions: Our study is one of the few studies evaluating the frequency of DMMD in Turkey. Although the prevalence of DMMD is low, comorbid diagnosis rates require detailed examination and multifaceted perspectives in the follow-up and treatment process

Phenological, morphological and genetic characterization of local grapevine (Vitis labrusca L.) genotypes grown in the Black Sea Region in Northern Turkey

Vitis labrusca has become widely naturalized in the Black Sea region of Northern Turkey. The aim of this work was to evaluate the morphological, phenological, and genetic characteristics of V. labrusca accessions grown in the Black Sea region during the last three growing seasons. Local genotypes were described according to the Organisation Internationale de la Vigne et du Vin (OIV) ampelographic descriptor, including shoot length (cm), mature leaf size, bunch length and width, berry length and width, berry weight, number of berries, Total Soluble Solids (TTS) and titratable acidity. Additionally, phenological data, including bud burst, flowering, veraison and harvest date, were recorded. The accessions were characterized at the molecular level, and genetic relationships were assessed by means of Inter Simple Sequence Repeat (ISSR) markers. Using 6 ISSR primers, seventy-seven bands were obtained, of which 69 were polymorphic with a mean polymorphic rate of 88.68%. These ISSR primers produced polymorphism information content (PIC) values ranging from 0.48 to 0.5. The genetic similarity ranged from 0.08 to 0.83 among the genotypes. According to the dendrogram based on the ISSR analysis, Accessions 8 and 5 were genetically related, with a coefficient of similarity of 0.83, while Accession 3 was the most distantly related genotype, with a coefficient of similarity of 0.08. These results demonstrated that ISSR markers can be used for genetic diversity analysis among V. labrusca genotypes. Our results also described characteristics of new V. labrusca genotypes that could be valuable for future Marker-Assisted Selection (MAS) and grapevine breeding.Key words: Diversity, foxy species, Inter-Simple Sequence Repeat markers (ISSRs), Breeding, polymorphis

Phenological, morphological and genetic characterization of local grapevine (Vitis labrusca L.) genotypes grown in the Black Sea Region in Northern Turkey

Vitis labrusca has become widely naturalized in the Black Sea region of Northern Turkey. The aim of this work was to evaluate the morphological, phenological, and genetic characteristics of V. labrusca accessions grown in the Black Sea region during the last three growing seasons. Local genotypes were described according to the Organisation Internationale de la Vigne et du Vin (OIV) ampelographic descriptor, including shoot length (cm), mature leaf size, bunch length and width, berry length and width, berry weight, number of berries, Total Soluble Solids (TTS) and titratable acidity. Additionally, phenological data, including bud burst, flowering, veraison and harvest date, were recorded. The accessions were characterized at the molecular level, and genetic relationships were assessed by means of Inter Simple Sequence Repeat (ISSR) markers. Using 6 ISSR primers, seventy-seven bands were obtained, of which 69 were polymorphic with a mean polymorphic rate of 88.68%. These ISSR primers produced polymorphism information content (PIC) values ranging from 0.48 to 0.5. The genetic similarity ranged from 0.08 to 0.83 among the genotypes. According to the dendrogram based on the ISSR analysis, Accessions 8 and 5 were genetically related, with a coefficient of similarity of 0.83, while Accession 3 was the most distantly related genotype, with a coefficient of similarity of 0.08. These results demonstrated that ISSR markers can be used for genetic diversity analysis among V. labrusca genotypes. Our results also described characteristics of new V. labrusca genotypes that could be valuable for future Marker-Assisted Selection (MAS) and grapevine breeding.Key words: Diversity, foxy species, Inter-Simple Sequence Repeat markers (ISSRs), Breeding, polymorphis

The level of anxiety in healthcare workers and their children during the COVID-19 pandemic

During the early stages of the COVID-19 pandemic, healthcare workers (HcWs) reported having high levels of psychological stress caused by the risk of being infected and infecting their families, social isolation, and inadequate protective equipment. This study aimed to determine the level of anxiety and related factors in HcWs and their children during the first phase of the COVID-19 pandemic in Turkey. Online questionnaires were sent to HcWs with children aged 8–18 years via e-mail and WhatsApp messages. In total, 144 HcWs and 135 of their children participated in this study. HcWs completed the State-Trait Anxiety Inventory state subscale (STAI-S) and the COVID-19 Risk Perception Scale. Their children completed the Screen for Child Anxiety-Related Emotional Disorders (SCARED). STAI-S and COVID-19 Risk Perception Scale scores of the HcWs in direct contact with COVID-19 patients were significantly higher than of those not in direct contact. Moreover, the children of HcWs that were in direct contact with COVID-19 patients had significantly higher SCARED subscale scores than those whose parents were not in direct contact. There was a significant correlation between the SCARED somatic/panic subscale scores and HcW STAI-S scores. The presence of a mental illness and direct contact with COVID-19 patients were the 2 main predictors of COVID-19 risk perception and the level of anxiety in HcWs. During the COVID-19 pandemic the children of HcWs were observed to be a mentally sensitive group, and preventive mental health programs should be developed

The effects of kaolin application on fruit development and fruit quality of 'Hayward' kiwifruit (Actinidia deliciosa A. Chev.)

The study was carried out to determine the effects of kaolin application on fruit development and fruit quality of 'Hayward' kiwifruit (Actinidia deliciosa A. Chev.) between the years of 2014 and 2015 in the research and the experimentation area of Recep Tayyip Erdoğan University, Faculty of Agriculture and Natural Sciences, Department of Horticulture. For this purpose, 3% of kaolin application was made twice at monthly intervals from the period of the small fruits. After the application, fruit width and length measurements were made in the labeled fruit of the four chosen directions of the tree in 15 days intervals until the harvest time. Furthermore, the effects of kaolin treatments on fruit firmness (N), titratable acidity amount (%), soluble solids (%), juice content (%) and leaf area were also determined. At the end of the trial, the effect of 3% of kaolin applications on fruit development and quality of 'Hayward' kiwifruit varieties is not statistically significant, however, it was detected that it provides a significant increase in fruit development

Potential And Future Of Small Fruits In The Eastern Black Sea Region

Doğu Karadeniz bölgesinde çay ve fındık dışında tarımsal üretim sınırlıdır. Ekolojik özellikler ve coğrafi yapı da bunu sınırlayan ana faktörlerdir. Bölgede miras nedeniyle bölünmüş arazilerin varlığı arazi boyutlarının bu bölgede küçülmesine neden olmuştur. Bunun sonucu, birim alandan daha çok gelir getirebilecek meyvelerin üretimi tercih edilmektedir. Bu anlamda üzümsü meyveler içerisinde bulunan; kivi, çilek, yaban mersini, kuşburnu ve böğürtlene bölgede ilgi artmıştır. Bölgede yeni bahçeler hızla kurulmaktadır. Ancak, bu ürünlerin üretimi ile ilgili teknik bilgi eksikliği, bahçe tesis maliyetlerinin yüksekliği ve pazarlama gibi çeşitli sorunlar da mevcuttur. Bu araştırmada Doğu Karadeniz Bölgesi'ndeki üzümsü meyvelerin mevcut durumu, potansiyeli ve geleceğe yönelik öneriler detaylı olarak ortaya konulmaktadırhazelnuts. Ecological characteristics and the geographical structure are the main factors limiting this. Heritage in the region because of the presence of divided land has led to a reduction in the size of the land. As a result, the production of fruit could bring more revenue per unit area is preferred. In this regard contained in the berries; kiwi, strawberries, blueberries, blackberries and rosehips increased interest in the region. New gardens in the region are quickly establishing. In this study, the current status of berries in the Eastern Black Sea Region, suggestions for potential and future are revealed in detai

Clinical Characteristics of Seizures in Epilepsy after Stroke: Prognosis and Treatment

Objectives:The aim of the present study was to determine demographic properties and risk factors for early- and late-onset seizures, as well as the effects of these factors on prognosis of patients with post-stroke epilepsy.Methods:Only patients with post-stroke epilepsy were included in this retrospective study. Demographic properties, stroke type, time interval between stroke and onset of seizure, neuroimaging, EEG characteristics, antiepileptic therapy, and prognosis were assessed.Results:Forty-five patients were included; 23 (51%) were female, and 22 (49%) were male. Mean age of study population was 64.6±1.3 (23–84). Sixty percent (n=27) of patients had early-onset seizures, and 40% (n=18) had late-onset seizures. Thirty-four patients had an ischemic stroke, and 11 had a hemorrhagic stroke. Most prevalent etiologic factor in the ischemic strokes was a cardioembolic stroke (15%). Middle-cerebral-artery infarcts were the most frequently observed arterial stroke region. In hemorrhagic stroke lobar, hemorrhages (54%) were more prevalent, and 87% of patients received monotherapy. Levetiracetam was the most commonly preferred medication, followed by carbamazepine, valproic acid, and oxcarbazepine, in descending order. Of the early-onset seizures, 63% were controlled with medication, as were 55% of the late-onset seizures. A statistically significant difference was observed between post-stroke localization and timing of seizure.Conclusion:Post-stroke seizures may present as early- or late-onset due to various underlying pathophysiologic mechanisms and prognostic properties. These seizures are affected by a variety of factors and are frequently controlled with monotherapy

The investigation of correlation between mean platelet volume and paroxysmal atrial fibrillation in patients with cryptogenic ischemic stroke

INTRODUCTION: The cause of ischemic stroke is thought to be undetermined in around a quarter of patients. Paroxysmal atrial fibrillation could be responsible for a significant number of cryptogenic stroke events. In recent studies, mean platelet volume (MPV) has been found to be increased during paroxysmal atrial fibrillation (PAF) attacks. In this study, we aimed to assess whether increased MPV in cryptogenic stroke patients could be prognostic of atrial fibrillation. METHODS: We analyzed cryptogenic ischemic stroke patients who were admitted to neurology clinic between July 2014-December 2015. Demographic features, blood tests, neuroimagings, standard electrocardiogram (EKG), 24 hours holter EKG, transthoracic echocardiography were all evaluated. RESULTS: 133 patients were included to the study. Atrial fibrillation (AF) was detected in 30 patients (22,6 %). MPV was not found to be increased statistically in patients with AF. DISCUSSION AND CONCLUSION: Although MPV has been found to be increased during paroxysmal atrial fibrillation (PAF) attacks in recent studies, we could not find any evidence that MPV could be prognostic of atrial fibrillation in cryptogenic ischemic stroke patients