Comparative Osteogenesis of Radiopaque Dicalcium Silicate Cement and White-Colored Mineral Trioxide Aggregate in a Rabbit Femur Model

The radiopaque dicalcium silicate cement (RDSC) displayed a shortened setting time and good biocompatibility. This study aimed to compare the regenerative potential of RDSC and white-colored mineral trioxide aggregate (WMTA) using a rabbit femur model. The animals were sacrificed at one, three and six months to accomplish histological and biochemical analyses. The results indicated that after one month of implantation, WMTA was associated with a greyish color alteration within its mass, while RDSC presented color stability even at six months. Histological assay with Masson’s Trichrome and Von Kossa stains showed the presence of newly formed bone surrounding the implanted sites in the rabbit femur. The histochemical data revealed that the RDSC group had significantly more bone regeneration than did the WMTA groups at three and six months. The conclusion drawn is that the encouraging results support the potential applications of RDSC as an improved alternative to WMTA for endodontic uses

Differential Impact of IL-10 Expression on Survival and Relapse between HPV16-Positive and -Negative Oral Squamous Cell Carcinomas

<div><p>Human papillomavirus (HPV) is a risk factor in a subset of oropharyngeal cancer; however, the contribution of HPV in the malignancy of oral squamous cell carcinomas (OSCC) is not fully understood in Taiwanese. Herein, 61 patients with no risk factors and 117 patients with one or more risk factors were enrolled in this study. HPV16/18 infection rate in non-smokers, non-drinkers and non-betel quid chewers was higher than their counterparts. The development of HPV-infected cancer has been shown to be associated with interleukin-10 (IL-10) expression. To this end, IL-10 mRNA expression in OSCC tumors was evaluated by real-time RT-PCR. Data showed that HPV-positive patients had higher IL-10 mRNA levels than in HPV-negative patients. Kaplan-Meier and Cox-regression analysis indicated that the prognostic significance of IL-10 mRNA on overall survival and relapse free survival was only observed in HPV-positive OSCC, but not in HPV-negative OSCC. Mechanistically, the elevation of IL-10 by E6 was responsible for increased colony formation and migration capability in OSCC cells. Therefore, we suggest that IL-10 induced by E6 promotes cell growth and migration capability and consequent poor survival and relapse in HPV-positive OSCC.</p> </div

Relationships between HPV16, HPV18 and HPV16/18 infection and clinical parameters in OSCC patients.

1<p>Other tumor sites includes plate (n = 1), gingival (n = 8), and lip (n = 9).</p>*<p>p<0.05;</p>#<p>p = 0.05.</p

Multivariate analysis of the influence of various parameters on overall survival (OS) and relapse free survival (RFS) in oral cancer patients according to gender.

<p>Multivariate analysis of the influence of various parameters on overall survival (OS) and relapse free survival (RFS) in oral cancer patients according to gender.</p

Kaplan-Meier analysis was used to assess the influence of IL-10 on OS and RFS.

<p>(A) in all study population, (B) in patients with HPV 16/18 infection compared to those without HPV 16/18 infection, (C) in patients with HPV 16/18 non-infection, and (D) in patients with HPV16/18 infection.</p

Comparison of IL-10 mRNA expression levels between HPV-positive and HPV–negative OSCC patients.

<p>Fourteen of 178 patients had both HPV16 and 18 infections.</p><p>IL-10 low: 0.6±0.8 (0.0–2.38); IL-10 high: 237.0±413.5 (2.39–1402.2).</p

IL-10 expression increases associated with HPV16/18 E6 is responsible for the migration capability of OSCC cell lines.

<p>(A) IL-10 expression in a panel of OSCC cell lines was evaluated by Western blotting; (B) GNM cells were knocked down by transfection of shHPV18 E6 and Tw2.6 cells were transiently transfected with HPV16 E6 cDNA plasmid for 48 hrs. β-actin was used as a protein loading control; (C) GNM cells were knocked down by transfection of shIL-10. Tw2.6 and SCC25 cells were co-transfected with HPV16 E6 cDNA plasmid, and then E6 and IL-10 expression were determined by Western blotting. β-actin was used as a protein loading control; (D) The migration and colony formation capability of GNM cells with or without transfection of shIL-10 was evaluated by transwell migration assay; and (E) E6 transfected-Tw2.6 and -SCC25 cells were co-transfected with two doses of shIL-10, and then the migration and colony formation capability of both cells was evaluated by transwell migration assay.</p