Structure and Function Relationships between ATPase Family, AAA Domain Containing Protein 5, Proliferating Cell Nuclear Antigen, and USP1-Associated Factor 1

The genome is constantly damaged by intracellular and extracellular factors. At sites of DNA damage, replication forks are stalled, leading monoubiquitination of proliferating cell nuclear antigen (PCNA). Monoubiquitination of PCNA promote the switch from regular high-fidelity polymerases to Y-family polymerases for bypassing damaged DNA. Prolonged replication by these polymerases may lead to increased mutagenesis, so tight regulation of this process is required. ATAD5 recruits a deubiquitinase complex consisting of ubiquitin-specific protease 1 (USP1) and USP1-associated factor 1 (UAF1) to control PCNA monoubiquitination. The mechanism by which ATAD5 and PCNA interact has been previously unexplored. We show through biochemical and structural studies that ATAD5 contains a non-canonical PCNA-interacting protein motif that interacts with PCNA in the low µM range. Our structural studies indicate that the binding of ATAD5’s PIP Box to PCNA is topologically conserved with respect to canonical PIP Boxes from other proteins. Furthermore, we detected weak interactions between ATAD5’s and UAF1’s protein interacting motifs. This suggests that ATAD5 acts as an adapter between PCNA and the UAF1-USP1 deubiquitinase complex. Characterization of these interactions will increase our understanding of DNA damage tolerance and may lead to the design of better cancer therapeutics

Improved Self-Adaptive Control Parameters in Differential Evolution Algorithm for Complex Numerical Optimization

芝浦工業大学201

Use Improved Differential Evolution Algorithms to Handle the Inverse Kinetics Problem for Robots with Residual Degrees of Freedom

In this study, the Self-adaptive strategy algorithm for controlling parameters in Differential Evolution algorithm (ISADE) improved from the Differential Evolution (DE) algorithm, as well as the upgraded version of the algorithms has been applied to solve the Inverse Kinetics (IK) problem for the redundant robot with 7 Degree of Freedom (DoF). The results were compared with 4 other algorithms of DE and Particle Swarm Optimization (PSO) as well as Pro-DE and Pro-PSO algorithms. These algorithms are tested in three different Scenarios for the motion trajectory of the end effector of in the workspace. In the first scenario, the IK results for a single point were obtained. 100 points randomly generated in the robot’s workspace was input parameters for Scenario 2, while Scenario 3 used 100 points located on a spline in the robot workspace. The algorithms were compared with each other based on the following criteria: execution time, endpoint distance error, number of generations required and especially quality of the joints’ variable found. The comparison results showed 2 main points: firstly, the ISADE algorithm gave much better results than the other DE and PSO algorithms based on the criteria of execution time, endpoint accuracy and generation number required. The second point is that when applying Pro-ISADE, Pro-DE and Pro-PSO algorithms, in addition to the ability to significantly improve the above parameters compared to the ISADE, DE and PSO algorithms, it also ensures the quality of solved joints’ values

Does more working women result in more single men?

The following report is going to discuss whether the relationship in the title exists. The theoretical approach suggests that both possibilities can happen due to the simple assumptions of the economic theories used in this research. Evidence shows a clear trend for a positive relationship between women’s working hours and the number of single men. However, the trend weakens over the time, possibly due to stabilisation of women’s wages more recently

Applying Improve Differential Evolution Algorithm for Solving Gait Generation Problem of Humanoid Robots

This chapter addresses an approach to generate 3D gait for humanoid robots. The proposed method considers gait generation matter as optimization problem with constraints. Firstly, trigonometric function is used to produce trial gait data for conducting simulation. By collecting the result, we build an approximation model to predict final status of the robot in locomotion, and construct optimization problem with constraints. In next step, we apply an improve differential evolution algorithm with Gauss distribution for solving optimization problem and achieve better gait data for the robot. This approach is validated using Kondo robot in a simulated dynamic environment. The 3D gait of the robot is compared to human in walk

BaAl1.4Si0.6O3.4N0.6:Eu2+ green phosphors’ application for improving luminous performance

The molten salt synthesis (MSS) method was used to effectively prepare green phosphors BaAl1.4Si0.6O3.4N0.6:Eu2+ (or BSON:Eu2+) via one homogeneous sphere-like morphology utilizing NaNO3 in the form of the reacting agent. The phosphors produced one wide stimulation spectrum between 250 and 460 nm, as well as a significant green emission has a maximum point at 510 nm owing to the 4f65d1-4f7 (8S7/2) shifts for Eu2+ ions. With illumination under 365 as well as 450 nm, the ideal discharge strengths for the specimen prepared utilizing melted salt would receive a boost of 17% and 13%, surpassing the specimen prepared utilizing the traditional solid-state reaction (SSR) approach. The abatement of concentration for the ions of Eu2+ from BSON:Eu2+ is 5 mol%. In addition, the interactivity of dipole-dipole would be the cause of said abatement. Heat abatement would be studied utilizing the formation coordinate method with abatement temperature reaching ∼200 oC. Elemental mapping as well as power-dispersing X-ray spectroscopy (EDS) spectra demonstrated that the expected BaAl1.4Si0.6O3.4N0.6:Eu2+ materials were formed

APPLICATION OF RICE HUSK WASTER IN WATER REMEDIATION IN VIETNAM: A REVIEW

Rice husk is a byproduct of rice milling that is commonly available in Vietnam due to the country's massive rice producing industry. While rice husk has traditionally been considered a waste product, recent research has shown that it has potential for use in wastewater treatment. This study comprehensively reviews a multitude of aspects regarding the application of rice husk waste in water treatment in Vietnam. The effectiveness of rice husk as an adsorbent material for removing heavy metals, organic compounds, and dyes from wastewater is highlighted. Besides, rice husk is even used for treating floodwater in Vietnam. The use of rice husk waste for water treatment has significant potential for both environmental and economic benefits for Vietnam. It provides a low-cost, sustainable alternative to traditional wastewater treatment methods and can help to address the country's ongoing water pollution challenges

Binding interactions of Peptide Aptamers

Peptide aptamers are short amino acid chains that are capable of binding specifically to ligands in the same way as their much larger counterparts, antibodies. Ligands of therapeutic interest that can be targeted are other peptide chains or loops located on the surface of protein receptors (e.g., GCPR), which take part in cell-to-cell communications either directly or via the intermediary of hormones or signalling molecules. To confer on aptamers the same sort of conformational rigidity that characterises an antibody binding site, aptamers are often constructed in the form of cyclic peptides, on the assumption that this will encourage stronger binding interactions than would occur if the aptamers were simply linear chains. However, no formal studies have been conducted to confirm the hypothesis that linear peptides will engage in stronger binding interactions with cyclic peptides than with other linear peptides. In this study, the interaction of a model cyclic decamer with a series of linear peptide constructs was compared with that of a linear peptide with the same sequence, showing that the cyclic configuration does confer benefits by increasing the strength of binding

The Relationship Between Corporate Social Responsibility and Employee Organizational Commitment in Multinational Corporations in Ho Chi Minh City

Based on the theoretical background of previous studies and the increasing context of personnel turnover in multinational companies, the authors conduct this study with the research objective to test and measure the impact of Corporate Social Responsibility (CSR) on employee’s organizational commitment through the effect of organizational identification and organizational trust. The combined qualitative and quantitative research methods were used to process and analyse the data. Research data was collected from 271 employees working in multinational corporations in Ho Chi Minh City. The study also discusses and provides some governance implications to help MNCs improve their social responsibility towards social and non-social stakeholders, employees, customers and the government as well as increase employee organizational engagement through identification and trust in the organization. Keywords: corporate social responsibility, employee organizational commitment, organizational identification, organizational trus