Effects of Different Pediatric Drugs on the Color Stability of Various Restorative Materials Applicable in Pediatric Dentistry

Background. The chronic recommendation of pediatric drugs could exhibit erosive and cariogenic problems. Objective. To evaluate the effects of different pediatric drugs on the color stability of various restorative materials. Methods. Five specimens (1mm x 3 mm) were prepared and immersed in ten different pediatric drugs and agitated every 8 hours daily for 2 min up to 1 week. Between immersion periods, the samples were stored in artificial saliva. After 1-week period, Delta Epsilon(*) values were calculated. Two-way ANOVA and Fisher's LSD test were used for statistical analysis at a level of p< 0.05. Results. Delta Epsilon(*) values were only significantly influenced by restorative material factor (p< 0.001) and varied in the range of 2.08 and 6.55 units for all drugs/estorative materials. The highest Delta Epsilon(*) was found in Ferrosanol B-composite (6.55 +/- 1.38) and the lowestonewas found inDolven-glass ionomer (2.08 +/- 0.40) pairwise. The most prominent Delta Epsilon(*) value elevations were obtained in composite material compared to the compomer and/or glass ionomers inMacrol, Ferrosanol B, and Ventolin (p < 0.001; for all) and also for other drugs (p< 0.05). Dolven exhibited significantly higher values compared to Augmentin (p - 0.021), Macrol (p - 0.018), and Ventolin (p - 0.013) in compomer group. Conclusion. The clinically perceptible color changes for tested composite/pediatric drug pairwise can be more problematic than compomer and glass ionomers in pediatric dentistry

Effects of Different Pediatric Drugs on the Color Stability of Various Restorative Materials Applicable in Pediatric Dentistry

Background. The chronic recommendation of pediatric drugs could exhibit erosive and cariogenic problems. Objective. To evaluate the effects of different pediatric drugs on the color stability of various restorative materials. Methods. Five specimens (1 mm × 3 mm) were prepared and immersed in ten different pediatric drugs and agitated every 8 hours daily for 2 min up to 1 week. Between immersion periods, the samples were stored in artificial saliva. After 1-week period, ΔE⁎ values were calculated. Two-way ANOVA and Fisher’s LSD test were used for statistical analysis at a level of p<0.05. Results. ΔE⁎ values were only significantly influenced by restorative material factor p<0.001 and varied in the range of 2.08 and 6.55 units for all drugs/restorative materials. The highest ΔE⁎ was found in Ferrosanol B-composite (6.55±1.38) and the lowest one was found in Dolven-glass ionomer (2.08±0.40) pairwise. The most prominent ΔE⁎ value elevations were obtained in composite material compared to the compomer and/or glass ionomers in Macrol, Ferrosanol B, and Ventolin (p<0.001; for all) and also for other drugs p<0.05. Dolven exhibited significantly higher values compared to Augmentin p=0.021, Macrol p=0.018, and Ventolin p=0.013 in compomer group. Conclusion. The clinically perceptible color changes for tested composite/pediatric drug pairwise can be more problematic than compomer and glass ionomers in pediatric dentistry

Chemical and molecular characterization of metabolites from Flavobacterium sp.

In this study, a Flavobacterium sp. is isolated from natural spring, and identified using molecular techniques. Extracellular and intracellular secondary metabolites are identified using solid phase microextraction gas chromatography-mass spectrometry and ultra performance liquid chromatography. Cytotoxic activity of the extracellular compounds produced by the Flavobacterium sp. and quercetin as the standard are measured using ECV304 human endothelial cells in vitro. Our results show that Flavobacterim sp. isolate has the highest percentage of similarity with Flavobacterium cheonhonense strain ARSA-15 (99%). Quercetin is detected as the major extracellular compound produced by the Flavobacterium sp. Methanol extract of Flavobacterium sp. resulted in a higher cell viability results when compared to DMSO extracts. Computational chemistry approach was used and it has been found that polar solvent (methanol) contributed to higher antioxidant activity. In conclusion, Flavobacterium sp. can be used to produce quercetin for industrial purposes

Nanomaterials and Nanorobotics in Dentistry: A Review

Nanotechnology is a branch of science focusing on the manipulation of materials measured on the nanoscale (size = 1-100 nm). Recent advances in the field of nanodentistry have resulted in the development of alternative treatment plans for common dental problems, bringing about a paradigm shift in dentistry. Nanorobots, also known as "nanites" or "nanomachines," are theoretical microscopic devices that may be used for the diagnosis and treatment of oral health problems. This paper aims to discuss the latest innovations in the field of nanodentistry

The Importance of Storage Time for Human Dental Pulp Cells Isolation

Objective: To compare the importance of storage time and the tooth type for isolation of dental pulp cells (DPCs) from extracted human teeth. Methods: 35 human teeth were used in this study. The teeth were stored in phosphate buffered saline (PBS) after extraction and divided into two groups randomly according to the time elapsed between extraction and isolation. In group one, the isolation was performed within 2 hours and in the other group it was performed 24 hours after extraction. Results: No significant differences between isolation time and total cell counts (p=0.483) and between isolation time and viable cells (p=0.341). No significant differences between the first molar and the premolar related cell counts and viable cells, but both teeth groups showed significant higher viability and had higher total cell amounts than third molars after isolation. Statistically significant correlations were found between age of donors and viable cells and viability after 24 hours isolation time. Conclusion: The immediate isolation of DPCs is not necessary after the tooth extraction. The tooth can be stored in PBS at room temperature up to twenty four hours after the extraction without a significant reduction in cell viability and counts. The cells obtained from younger donors might have more chance for more viability even if storage time was extended. Premolars and first molars were better donors than the third molars for DPCs isolations and the high number of success revascularization rate in premolars with necrotic immature premolars might be because of their high cell viability potentials

Hereditary spastic paraplegia with recessive trait caused by mutation in KLC4 gene

We report an association between a new causative gene and spastic paraplegia, which is a genetically heterogeneous disorder. Clinical phenotyping of one consanguineous family followed by combined homozygosity mapping and whole-exome sequencing analysis. Three patients from the same family shared common features of progressive complicated spastic paraplegia. They shared a single homozygous stretch area on chromosome 6. Whole-exome sequencing revealed a homozygous mutation (c.853_871del19) in the gene coding the kinesin light chain 4 protein (KLC4). Meanwhile, the unaffected parents and two siblings were heterozygous and one sibling was homozygous wild type. The 19 bp deletion in exon 6 generates a stop codon and thus a truncated messenger RNA and protein. The association of a KLC4 mutation with spastic paraplegia identifies a new locus for the disease