New Approaches of Ovarian Tissue Cryopreservation from Domestic Animal Species

International audienc

The Use of Chitooligosaccharides in Cryopreservation: Discussion of Concept and First Answers from DSC Thermal Analysis

The use of dimethyl sulfoxide (Me2SO) as a cryoprotectant agent (CPA) is controversial. Indeed, this cryoprotectant agent (CPA) is cytotoxic and potentially mutagenic. Therefore, other cryoprotectants must be used to reduce the proportion of Me2SO in slow-freezing solutions. In this chapter, we propose to present the first evaluation of new non-penetrating cryoprotectants: the chitooligosaccharides (COS). These molecules are chitosan oligomers, which are biocompatible, antioxidant, and bacteriostatic. We first review the use of saccharides through cryopreservation processes. We question the possibility to reduce penetrating CPA during slow-freezing procedures. We propose to use COS as extracellular CPA to reduce the use of Me2SO. We question the biocompatibility of COS on mouse embryos through the analysis of the cells’ development. Next, we evaluate these molecules in slow-freezing solutions with a reduced quantity of Me2SO. Our experimental approach is a physical method often used to characterize slow-freezing solutions. Differential scanning calorimetry (DSC) allows to evaluate the crystallization and melting processes, the amount of crystallized water, and the equilibrium temperature and consequently to evaluate the impact of different cryoprotectants. This study gives a better understanding on how slow-freezing protocols could be improved with extracellular CPA

Applications des tests génétiques au dépistage des affections génétiques chez le chien

LYON1-BU Santé (693882101) / SudocSudocFranceF

La contraception chimique chez la chienne et la chatte (réalisation d'une enquête sur les pratiques en clientèle vétérinaire)

LYON1-BU Santé (693882101) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF

Les Troubles de la lactation chez la chienne

LYON1-BU Santé (693882101) / SudocSudocFranceF

Diagnostic de gestation précoce chez la chienne (comparaison de deux méthodes : l'échographie et le dosage de la relaxine)

LYON1-BU Santé (693882101) / SudocSudocFranceF

La Prévention des troubles du comportement chez le chiot à l'élevage

LYON1-BU Santé (693882101) / SudocSudocFranceF

Démarche diagnostique lors d'infécondité en élevage félin

LYON1-BU Santé (693882101) / SudocSudocFranceF

Apports de la cytométrie en flux dans la reproduction des carnivores domestiques

LYON1-BU Santé (693882101) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF

Démarches diagnostique et thérapeutique des affections prostatiques chez le chien

LYON1-BU Santé (693882101) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF