The effect of excessive dietary calcium upon the absorption and utilization of selenium-75, molybdenum-99, copper-64 and iron-59 in swine

The objectives of this study were: 1. To determine the absorption and utilization of Selenium-75, Molybdenum-99, Copper-6H and Iron-59 when feeding excessive calcium to growing swine. 2. To further determine the distribution of the radioactive elements in the tissues of these animals. 3. To determine growth rate, feed consumption and feed efficiency when feeding excessive levels of calcium. These isotopes were selected for this study because of their properties, which made it possible to employ the multiple dosing technique . Se-75 and Mo-99 were used together because both are gamma emitters and Se-75 has a half-life of 120 days whereas Ho-99 has a halflife of 2.8 days. Likewise copper and iron were used simultaneously since Fe-59 has a half-life of 45.1 days and Cu-64 has a half-life of 12.8 hours and both are gamma emitters. Each of the samples could be counted twice and the short half-life isotope corrected for decay and determined by difference

The e-Business Readiness Composite Indicator for 2003. A Pilot Study

Abstract not availableJRC.G-Institute for the Protection and the Security of the Citizen (Ispra

Phylogeography of Japanese encephalitis virus:genotype is associated with climate

The circulation of vector-borne zoonotic viruses is largely determined by the overlap in the geographical distributions of virus-competent vectors and reservoir hosts. What is less clear are the factors influencing the distribution of virus-specific lineages. Japanese encephalitis virus (JEV) is the most important etiologic agent of epidemic encephalitis worldwide, and is primarily maintained between vertebrate reservoir hosts (avian and swine) and culicine mosquitoes. There are five genotypes of JEV: GI-V. In recent years, GI has displaced GIII as the dominant JEV genotype and GV has re-emerged after almost 60 years of undetected virus circulation. JEV is found throughout most of Asia, extending from maritime Siberia in the north to Australia in the south, and as far as Pakistan to the west and Saipan to the east. Transmission of JEV in temperate zones is epidemic with the majority of cases occurring in summer months, while transmission in tropical zones is endemic and occurs year-round at lower rates. To test the hypothesis that viruses circulating in these two geographical zones are genetically distinct, we applied Bayesian phylogeographic, categorical data analysis and phylogeny-trait association test techniques to the largest JEV dataset compiled to date, representing the envelope (E) gene of 487 isolates collected from 12 countries over 75 years. We demonstrated that GIII and the recently emerged GI-b are temperate genotypes likely maintained year-round in northern latitudes, while GI-a and GII are tropical genotypes likely maintained primarily through mosquito-avian and mosquito-swine transmission cycles. This study represents a new paradigm directly linking viral molecular evolution and climate

Probing RS scenarios of flavour at LHC via leptonic channels

We study a purely leptonic signature of the Randall-Sundrum scenario with Standard Model fields in the bulk at LHC: the contribution from the exchange of Kaluza-Klein (KK) excitations of gauge bosons to the clear Drell-Yan reaction. We show that this contribution is detectable (even with the low luminosities of the LHC initial regime) for KK masses around the TeV scale and for sufficiently large lepton couplings to KK gauge bosons. Such large couplings can be compatible with ElectroWeak precision data on the Zff coupling in the framework of the custodial O(3) symmetry recently proposed, for specific configurations of lepton localizations (along the extra dimension). These configurations can simultaneously reproduce the correct lepton masses, while generating acceptably small Flavour Changing Neutral Current (FCNC) effects. This LHC phenomenological analysis is realistic in the sense that it is based on fermion localizations which reproduce all the quark/lepton masses plus mixing angles and respect FCNC constraints in both the hadron and lepton sectors.Comment: 15 pages, 6 Figures, Latex fil

Proposed Search for a00(980)−f0(980)a^0_0(980)-f_0(980) Mixing in Polarization Phenomena

The $K^+$ and $K^0$ meson mass difference induces the mixing of the $a^0_0(980)$ and $f_0(980)$ resonances, the amplitude of which, between the $K^+K^-$ and $K^0\bar K^0$ thresholds, is large in magnitude, of the order of $m_{K}\sqrt{m^2_{K^0}-m^2_{K^+}}\approx\sqrt\alpha m^2_K$, and possesses the phase sharply varying by about 90$^\circ$. We suggest performing the polarized target experiments on the reaction $\pi^-p\to\eta\pi^0n$ at high energy in which the fact of the existence of $a^0_0(980)-f_0(980)$ mixing can be unambiguously and very easily established through the presence of a strong jump in the azimuthal asymmetry of the $\eta\pi^0$ $S$ wave production cross section near the $K\bar K$ thresholds. The presented estimates of the polarization effect to be expected in experiment are to a great extent model independent.Comment: RevTeX, 9 pages, 1 figure. A number of typographical and grammatical errors correcte

Cosmic ray tests of the D0 preshower detector

The D0 preshower detector consists of scintillator strips with embedded wavelength-shifting fibers, and a readout using Visible Light Photon Counters. The response to minimum ionizing particles has been tested with cosmic ray muons. We report results on the gain calibration and light-yield distributions. The spatial resolution is investigated taking into account the light sharing between strips, the effects of multiple scattering and various systematic uncertainties. The detection efficiency and noise contamination are also investigated.Comment: 27 pages, 24 figures, submitted to NIM

Simultaneous quantification of 12 different nucleotides and nucleosides released from renal epithelium and in human urine samples using ion-pair reversed-phase HPLC

Nucleotides and nucleosides are not only involved in cellular metabolism but also act extracellularly via P1 and P2 receptors, to elicit a wide variety of physiological and pathophysiological responses through paracrine and autocrine signalling pathways. For the first time, we have used an ion-pair reversed-phase high-performance liquid chromatography ultraviolet (UV)-coupled method to rapidly and simultaneously quantify 12 different nucleotides and nucleosides (adenosine triphosphate, adenosine diphosphate, adenosine monophosphate, adenosine, uridine triphosphate, uridine diphosphate, uridine monophosphate, uridine, guanosine triphosphate, guanosine diphosphate, guanosine monophosphate, guanosine): (1) released from a mouse renal cell line (M1 cortical collecting duct) and (2) in human biological samples (i.e., urine). To facilitate analysis of urine samples, a solid-phase extraction step was incorporated (overall recovery rate ? 98 %). All samples were analyzed following injection (100 ?l) into a Synergi Polar-RP 80 Å (250 × 4.6 mm) reversed-phase column with a particle size of 10 ?m, protected with a guard column. A gradient elution profile was run with a mobile phase (phosphate buffer plus ion-pairing agent tetrabutylammonium hydrogen sulfate; pH 6) in 2-30 % acetonitrile (v/v) for 35 min (including equilibration time) at 1 ml min(-1) flow rate. Eluted compounds were detected by UV absorbance at 254 nm and quantified using standard curves for nucleotide and nucleoside mixtures of known concentration. Following validation (specificity, linearity, limits of detection and quantitation, system precision, accuracy, and intermediate precision parameters), this protocol was successfully and reproducibly used to quantify picomolar to nanomolar concentrations of nucleosides and nucleotides in isotonic and hypotonic cell buffers that transiently bathed M1 cells, and urine samples from normal subjects and overactive bladder patients

Minimal Universal Extra Dimensions in CalcHEP/CompHEP

We present an implementation of the model of minimal universal extra dimensions (MUED) in CalcHEP/CompHEP. We include all level-1 and level-2 Kaluza-Klein (KK) particles outside the Higgs sector. The mass spectrum is automatically calculated at one loop in terms of the two input parameters in MUED: the radius of the extra dimension and the cut-off scale of the model. We implement both the KK number conserving and the KK number violating interactions of the KK particles. We also account for the proper running of the gauge coupling constants above the electroweak scale. The implementation has been extensively cross-checked against known analytical results in the literature and numerical results from other programs. Our files are publicly available and can be used to perform various automated calculations within the MUED model.Comment: 32 pages, 4 figures, 6 tables, invited contribution for New Journal of Physics Focus Issue on 'Extra Space Dimensions', the model file can be downloaded from http://home.fnal.gov/~kckong/mued