KatG fromSalmonellaTyphimurium is a peroxynitritase

AbstractPathogenic bacteria elicit protective responses to oxidative and nitrosative stresses. Although such responses are generally distinct, it was recently reported in Mycobacterium tuberculosis that catalase–peroxidase (KatG), a classical defence against peroxides, also exhibits peroxynitritase activity. Here, the katG gene from Salmonella Typhimurium was cloned and protein purified and characterised. An increase in the rate of decomposition of peroxynitrite was observed for KatG from the enterobacterium with a second-order rate constant of 4.2×104M−1s−1 at pH 7.4, 25°C. This enzyme was able to reduce dihydrorhodamine oxidation by peroxynitrite to ∼83%. Given the peroxynitritase activity demonstrated here it is likely that KatG may play a wider role in the detoxification of oxidative stresses than previously thought

KatG fromSalmonellaTyphimurium is a peroxynitritase

AbstractPathogenic bacteria elicit protective responses to oxidative and nitrosative stresses. Although such responses are generally distinct, it was recently reported in Mycobacterium tuberculosis that catalase–peroxidase (KatG), a classical defence against peroxides, also exhibits peroxynitritase activity. Here, the katG gene from Salmonella Typhimurium was cloned and protein purified and characterised. An increase in the rate of decomposition of peroxynitrite was observed for KatG from the enterobacterium with a second-order rate constant of 4.2×104M−1s−1 at pH 7.4, 25°C. This enzyme was able to reduce dihydrorhodamine oxidation by peroxynitrite to ∼83%. Given the peroxynitritase activity demonstrated here it is likely that KatG may play a wider role in the detoxification of oxidative stresses than previously thought

Does It Really Matter If You Recognize Who I Am? The Implications of Identity Comprehension for Individuals in Work Teams †

A field study of 179 employees in an investment technology firm explores the relationship between identity comprehension and individual work outcomes. Identity comprehension is defined as the degree to which the relative importance of one’s identities is recognized by important others. Results show that individuals with higher levels of identity comprehension in work teams are likely to have higher levels of creativity and satisfaction and lower levels of absenteeism