Testing and Design of Bolted Connections in Cold Formed Steel Sections

A detailed analysis of the strength and rigidity of bolted connections in cold formed steel has been carried out. Design expressions are proposed that improve on the existing equations for bearing strength, and for the first time, quantify the rigidity of such connections. The effects on the design of bolted joints are described and it is shown how, by incorporating the proposed expressions, it is now possible to select the correct semirigid joint and achieve economy without having to resort to testing

Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi.

Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches endogenous levels. Here, we developed an endogenous gene knockdown/rescue strategy that combines RNAi selectivity with a highly efficient CRISPR directed recombinant Adeno-Associated Virus (rAAV) mediated gene targeting approach to introduce allele-specific mutations plus an allele-selective siRNA Sensitive (siSN) site that allows for studying gene mutations while maintaining endogenous expression and regulation of the gene of interest. CRISPR/Cas9 plus rAAV targeted gene-replacement and introduction of allele-specific RNAi sensitivity mutations in the CDK2 and CDK1 genes resulted in a >85% site-specific recombination of Neo-resistant clones versus ∼8% for rAAV alone. RNAi knockdown of wild type (WT) Cdk2 with siWT in heterozygotic knockin cells resulted in the mutant Cdk2 phenotype cell cycle arrest, whereas allele specific knockdown of mutant CDK2 with siSN resulted in a wild type phenotype. Together, these observations demonstrate the ability of CRISPR plus rAAV to efficiently recombine a genomic locus and tag it with a selective siRNA sequence that allows for allele-selective phenotypic assays of the gene of interest while it remains expressed and regulated under endogenous control mechanisms

First-Principles Structural, Mechanical, and Thermodynamic Calculations of the Negative Thermal Expansion Compound Zr2(WO4)(PO4)2

The negative thermal expansion (NTE) material Zr2(WO4)(PO4)2 has been investigated for the first time within the framework of the density functional perturbation theory (DFPT). The structural, mechanical, and thermodynamic properties of this material have been predicted using the Perdew, Burke and Ernzerhof for solid (PBEsol) exchange–correlation functional, which showed superior accuracy over standard functionals in previous computational studies of the NTE material α-ZrW2O8. The bulk modulus calculated for Zr2(WO4)(PO4)2 using the Vinet equation of state at room temperature is K0 = 63.6 GPa, which is in close agreement with the experimental estimate of 61.3(8) at T = 296 K. The computed mean linear coefficient of thermal expansion is −3.1 × 10–6 K−1 in the temperature range ∼0–70 K, in line with the X-ray diffraction measurements. The mean Grüneisen parameter controlling the thermal expansion of Zr2(WO4)(PO4)2 is negative below 205 K, with a minimum of −2.1 at 10 K. The calculated standard molar heat capacity and entropy are CP0 = 287.6 and S0 = 321.9 J·mol–1·K–1, respectively. The results reported in this study demonstrate the accuracy of DFPT/PBEsol for assessing or predicting the relationship between structural and thermomechanical properties of NTE materials

Comparing Food and Cash Transfers to the Ultra-Poor in Bangladesh

"Bangladesh has some social safety net programs that transfer food to the poor, some that transfer cash, and some that provide a combination of both. This study evaluates the relative impacts of food and cash transfers on food security and livelihood outcomes among the ultra poor in Bangladesh. The programs impacts are evaluated according to various measures, including how well transfers are delivered; which transfers beneficiaries prefer; how accurately the programs target the extremely poor; effects on food security, livelihoods, and women’s empowerment; and cost effectiveness. The report identifies what has and has not worked in food and cash transfers and recommends ways of improving these programs. This study will be valuable to policymakers and others concerned with poverty reduction in Bangladesh and elsewhere." from textCash transfers, cost effectiveness, food security, Poverty, Poverty reduction, safety net programs, women empowerment,

Multidisciplinary research from ERTS-1 data

There are no author-identified significant results in this report

Use of ERTS-1 data: Summary report of work on ten tasks

The author has identified the following significant results. Depth mapping's for a portion of Lake Michigan and at the Little Bahama Bank test site have been verified by use of navigation charts and on-site visits. A thirteen category recognition map of Yellowstone Park has been prepared. Model calculation of atmospheric effects for various altitudes have been prepared. Radar, SLAR, and ERTS-1 data for flooded areas of Monroe County, Michigan are being studied. Water bodies can be reliably recognized and mapped using maximum likelihood processing of ERTS-1 digital data. Wetland mapping has been accomplished by slicing of single band and/or ratio processing of two bands for a single observation date. Both analog and digital processing have been used to map the Lake Ontario basin using ERTS-1 data. Operating characteristic curves were developed for the proportion estimation algorithm to determine its performance in the measurement of surface water area. The signal in band MSS-5 was related to sediment content of waters by modelling approach and by relating surface measurements of water to processed ERTS data. Radiance anomalies in ERTS-1 data could be associated with the presence of oil on water in San Francisco Bay, but the anomalies were of the same order as those caused by variations in sediment concentration and tidal flushing

Explaining the entropy excess in clusters and groups of galaxies without additional heating

The X-ray luminosity and temperature of clusters and groups of galaxies do not scale in a self-similar manner. This has often been interpreted as a sign that the intracluster medium has been substantially heated by non-gravitational sources. In this paper, we propose a simple model which, instead, uses the properties of galaxy formation to explain the observations. Drawing on available observations, we show that there is evidence that the efficiency of galaxy formation was higher in groups than in clusters. If confirmed, this would deplete the low-entropy gas in groups, increase their central entropy and decrease their X-ray luminosity. A simple, empirical, hydrostatic model appears to match both the luminosity-temperature relation of clusters and properties of their internal structure as well.Comment: 5 pages, 4 figures, accepted in ApJL; added one reference, otherwise unchange

Summary report of work on ten tasks

There are no author-identified significant results in this report

Cosmological Simulations of the Preheating Scenario for Galaxy Cluster Formation: Comparison to Analytic Models and Observations

We perform a set of non--radiative cosmological simulations of a preheated intracluster medium in which the entropy of the gas was uniformly boosted at high redshift. The results of these simulations are used first to test the current analytic techniques of preheating via entropy input in the smooth accretion limit. When the unmodified profile is taken directly from simulations, we find that this model is in excellent agreement with the results of our simulations. This suggests that preheated efficiently smoothes the accreted gas, and therefore a shift in the unmodified profile is a good approximation even with a realistic accretion history. When we examine the simulation results in detail, we do not find strong evidence for entropy amplification, at least for the high-redshift preheating model adopted here. In the second section of the paper, we compare the results of the preheating simulations to recent observations. We show -- in agreement with previous work -- that for a reasonable amount of preheating, a satisfactory match can be found to the mass-temperature and luminosity-temperature relations. However -- as noted by previous authors -- we find that the entropy profiles of the simulated groups are much too flat compared to observations. In particular, while rich clusters converge on the adiabatic self--similar scaling at large radius, no single value of the entropy input during preheating can simultaneously reproduce both the core and outer entropy levels. As a result, we confirm that the simple preheating scenario for galaxy cluster formation, in which entropy is injected universally at high redshift, is inconsistent with observations.Comment: 11 pages, 13 figures, accepted for publication in Ap