1,489 research outputs found
Ultrafast Structural Dynamics of BlsA, a Photoreceptor from the Pathogenic Bacterium Acinetobacter baumannii
Acinetobacter baumannii is an important human pathogen that can form biofilms and persist under harsh environmental conditions. Biofilm formation and virulence are modulated by blue light, which is thought to be regulated by a BLUF protein, BlsA. To understand the molecular mechanism of light sensing, we have used steady-state and ultrafast vibrational spectroscopy to compare the photoactivation mechanism of BlsA to the BLUF photosensor AppA from Rhodobacter sphaeroides. Although similar photocycles are observed, vibrational data together with homology modeling identify significant differences in the β5 strand in BlsA caused by photoactivation, which are proposed to be directly linked to downstream signaling
T-cell derived acetylcholine aids host defenses during enteric bacterial infection with Citrobacter rodentium.
The regulation of mucosal immune function is critical to host protection from enteric pathogens but is incompletely understood. The nervous system and the neurotransmitter acetylcholine play an integral part in host defense against enteric bacterial pathogens. Here we report that acetylcholine producing-T-cells, as a non-neuronal source of ACh, were recruited to the colon during infection with the mouse pathogen Citrobacter rodentium. These ChAT+ T-cells did not exclusively belong to one Th subset and were able to produce IFNγ, IL-17A and IL-22. To interrogate the possible protective effect of acetylcholine released from these cells during enteric infection, T-cells were rendered deficient in their ability to produce acetylcholine through a conditional gene knockout approach. Significantly increased C. rodentium burden was observed in the colon from conditional KO (cKO) compared to WT mice at 10 days post-infection. This increased bacterial burden in cKO mice was associated with increased expression of the cytokines IL-1β, IL-6, and TNFα, but without significant changes in T-cell and ILC associated IL-17A, IL-22, and IFNγ, or epithelial expression of antimicrobial peptides, compared to WT mice. Despite the increased expression of pro-inflammatory cytokines during C. rodentium infection, inducible nitric oxide synthase (Nos2) expression was significantly reduced in intestinal epithelial cells of ChAT T-cell cKO mice 10 days post-infection. Additionally, a cholinergic agonist enhanced IFNγ-induced Nos2 expression in intestinal epithelial cell in vitro. These findings demonstrated that acetylcholine, produced by specialized T-cells that are recruited during C. rodentium infection, are a key mediator in host-microbe interactions and mucosal defenses
BLUF Domain Function Does Not Require a Metastable Radical Intermediate State
BLUF
(blue light using flavin) domain proteins are an important
family of blue light-sensing proteins which control a wide variety
of functions in cells. The primary light-activated step in the BLUF
domain is not yet established. A number of experimental and theoretical
studies points to a role for photoinduced electron transfer (PET)
between a highly conserved tyrosine and the flavin chromophore to
form a radical intermediate state. Here we investigate the role of
PET in three different BLUF proteins, using ultrafast broadband transient
infrared spectroscopy. We characterize and identify infrared active
marker modes for excited and ground state species and use them to
record photochemical dynamics in the proteins. We also generate mutants
which unambiguously show PET and, through isotope labeling of the
protein and the chromophore, are able to assign modes characteristic
of both flavin and protein radical states. We find that these radical
intermediates are not observed in two of the three BLUF domains studied,
casting doubt on the importance of the formation of a population of
radical intermediates in the BLUF photocycle. Further, unnatural amino
acid mutagenesis is used to replace the conserved tyrosine with fluorotyrosines,
thus modifying the driving force for the proposed electron transfer
reaction; the rate changes observed are also not consistent with a
PET mechanism. Thus, while intermediates of PET reactions can be observed
in BLUF proteins they are not correlated with photoactivity, suggesting
that radical intermediates are not central to their operation. Alternative
nonradical pathways including a keto–enol tautomerization induced
by electronic excitation of the flavin ring are considered
SUSY Stops at a Bump
We discuss collider signatures of the "natural supersymmetry" scenario with
baryon-number violating R-parity violation. We argue that this is one of the
few remaining viable incarnations of weak scale supersymmetry consistent with
full electroweak naturalness. We show that this intriguing and challenging
scenario contains distinctive LHC signals, resonances of hard jets in
conjunction with relatively soft leptons and missing energy, which are easily
overlooked by existing LHC searches. We propose novel strategies for
distinguishing these signals above background, and estimate their potential
reach at the 8 TeV LHC. We show that other multi-lepton signals of this
scenario can be seen by currently existing searches with increased statistics,
but these opportunities are more spectrum-dependent.Comment: 23 pages, 7 figures, 3 tables. V2: spectrum discussion corrected,
most of the changes are in Sec. 2. Benchmarks, analysis and conclusions
unchanged. References adde
Supersymmetry with Light Stops
Recent LHC data, together with the electroweak naturalness argument, suggest
that the top squarks may be significantly lighter than the other sfermions. We
present supersymmetric models in which such a split spectrum is obtained
through "geometries": being "close to" electroweak symmetry breaking implies
being "away from" supersymmetry breaking, and vice versa. In particular, we
present models in 5D warped spacetime, in which supersymmetry breaking and
Higgs fields are located on the ultraviolet and infrared branes, respectively,
and the top multiplets are localized to the infrared brane. The hierarchy of
the Yukawa matrices can be obtained while keeping near flavor degeneracy
between the first two generation sfermions, avoiding stringent constraints from
flavor and CP violation. Through the AdS/CFT correspondence, the models can be
interpreted as purely 4D theories in which the top and Higgs multiplets are
composites of some strongly interacting sector exhibiting nontrivial dynamics
at a low energy. Because of the compositeness of the Higgs and top multiplets,
Landau pole constraints for the Higgs and top couplings apply only up to the
dynamical scale, allowing for a relatively heavy Higgs boson, including m_h =
125 GeV as suggested by the recent LHC data. We analyze electroweak symmetry
breaking for a well-motivated subset of these models, and find that fine-tuning
in electroweak symmetry breaking is indeed ameliorated. We also discuss a flat
space realization of the scenario in which supersymmetry is broken by boundary
conditions, with the top multiplets localized to a brane while other matter
multiplets delocalized in the bulk.Comment: 27 pages, 7 figure
Infarction in the territory of the anterior cerebral artery: clinical study of 51 patients
<p>Abstract</p> <p>Background</p> <p>Little is known about clinical features and prognosis of patients with ischaemic stroke caused by infarction in the territory of the anterior cerebral artery (ACA). This single centre, retrospective study was conducted with the following objectives: a) to describe the clinical characteristics and short-term outcome of stroke patients with ACA infarction as compared with that of patients with ischaemic stroke due to middle cerebral artery (MCA) and posterior cerebral artery (PCA) infarctions, and b) to identify predictors of ACA stroke.</p> <p>Methods</p> <p>Fifty-one patients with ACA stroke were included in the "Sagrat Cor Hospital of Barcelona Stroke Registry" during a period of 19 years (1986–2004). Data from stroke patients are entered in the stroke registry following a standardized protocol with 161 items regarding demographics, risk factors, clinical features, laboratory and neuroimaging data, complications and outcome. The characteristics of these 51 patients with ACA stroke were compared with those of the 1355 patients with MCA infarctions and 232 patients with PCA infarctions included in the registry.</p> <p>Results</p> <p>Infarctions of the ACA accounted for 1.3% of all cases of stroke (<it>n </it>= 3808) and 1.8% of cerebral infarctions (<it>n </it>= 2704). Stroke subtypes included cardioembolic infarction in 45.1% of patients, atherothrombotic infarction in 29.4%, lacunar infarct in 11.8%, infarct of unknown cause in 11.8% and infarction of unusual aetiology in 2%. In-hospital mortality was 7.8% (<it>n </it>= 4). Only 5 (9.8%) patients were symptom-free at hospital discharge. Speech disturbances (odds ratio [OR] = 0.48) and altered consciousness (OR = 0.31) were independent variables of ACA stroke in comparison with MCA infarction, whereas limb weakness (OR = 9.11), cardioembolism as stroke mechanism (OR = 2.49) and sensory deficit (OR = 0.35) were independent variables associated with ACA stroke in comparison with PCA infarction.</p> <p>Conclusion</p> <p>Cardioembolism is the main cause of brain infarction in the territory of the ACA. Several clinical features are more frequent in stroke patients with ACA infarction than in patients with ischaemic stroke due to infarction in the MCA and PCA territories.</p
Fitting Neutrino Physics with a U(1)_R Lepton Number
We study neutrino physics in the context of a supersymmetric model where a
continuous R-symmetry is identified with the total Lepton Number and one
sneutrino can thus play the role of the down type Higgs. We show that
R-breaking effects communicated to the visible sector by Anomaly Mediation can
reproduce neutrino masses and mixing solely via radiative contributions,
without requiring any additional degree of freedom. In particular, a relatively
large reactor angle (as recently observed by the Daya Bay collaboration) can be
accommodated in ample regions of the parameter space. On the contrary, if the
R-breaking is communicated to the visible sector by gravitational effects at
the Planck scale, additional particles are necessary to accommodate neutrino
data.Comment: 19 pages, 3 figures; v2: references added, constraints updated,
overall conclusions unchange
Flavor Mediation Delivers Natural SUSY
If supersymmetry (SUSY) solves the hierarchy problem, then naturalness
considerations coupled with recent LHC bounds require non-trivial superpartner
flavor structures. Such "Natural SUSY" models exhibit a large mass hierarchy
between scalars of the third and first two generations as well as degeneracy
(or alignment) among the first two generations. In this work, we show how this
specific beyond the standard model (SM) flavor structure can be tied directly
to SM flavor via "Flavor Mediation". The SM contains an anomaly-free SU(3)
flavor symmetry, broken only by Yukawa couplings. By gauging this flavor
symmetry in addition to SM gauge symmetries, we can mediate SUSY breaking via
(Higgsed) gauge mediation. This automatically delivers a natural SUSY spectrum.
Third-generation scalar masses are suppressed due to the dominant breaking of
the flavor gauge symmetry in the top direction. More subtly, the
first-two-generation scalars remain highly degenerate due to a custodial U(2)
symmetry, where the SU(2) factor arises because SU(3) is rank two. This
custodial symmetry is broken only at order (m_c/m_t)^2. SUSY gauge coupling
unification predictions are preserved, since no new charged matter is
introduced, the SM gauge structure is unaltered, and the flavor symmetry treats
all matter multiplets equally. Moreover, the uniqueness of the anomaly-free
SU(3) flavor group makes possible a number of concrete predictions for the
superpartner spectrum.Comment: 17 pages, 7 figures, 2 tables. v2 references added, minor changes to
flavor constraints and a little discussion adde
Calculations of collisions between cold alkaline earth atoms in a weak laser field
We calculate the light-induced collisional loss of laser-cooled and trapped
magnesium atoms for detunings up to 50 atomic linewidths to the red of the
^1S_0-^1P_1 cooling transition. We evaluate loss rate coefficients due to both
radiative and nonradiative state-changing mechanisms for temperatures at and
below the Doppler cooling temperature. We solve the Schrodinger equation with a
complex potential to represent spontaneous decay, but also give analytic models
for various limits. Vibrational structure due to molecular photoassociation is
present in the trap loss spectrum. Relatively broad structure due to absorption
to the Mg_2 ^1Sigma_u state occurs for detunings larger than about 10 atomic
linewidths. Much sharper structure, especially evident at low temperature,
occurs even at smaller detunings due to of Mg_2 ^1Pi_g absorption, which is
weakly allowed due to relativistic retardation corrections to the forbidden
dipole transition strength. We also perform model studies for the other
alkaline earth species Ca, Sr, and Ba and for Yb, and find similar qualitative
behavior as for Mg.Comment: 20 pages, RevTex, 13 eps figures embedde
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