64 research outputs found

    Visualizing classification of natural video sequences using sparse, hierarchical models of cortex.

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    Recent work on hierarchical models of visual cortex has reported state-of-the-art accuracy on whole-scene labeling using natural still imagery. This raises the question of whether the reported accuracy may be due to the sophisticated, non-biological back-end supervised classifiers typically used (support vector machines) and/or the limited number of images used in these experiments. In particular, is the model classifying features from the object or the background? Previous work (Landecker, Brumby, et al., COSYNE 2010) proposed tracing the spatial support of a classifier’s decision back through a hierarchical cortical model to determine which parts of the image contributed to the classification, compared to the positions of objects in the scene. In this way, we can go beyond standard measures of accuracy to provide tools for visualizing and analyzing high-level object classification. We now describe new work exploring the extension of these ideas to detection of objects in video sequences of natural scenes

    Real-Time Detection of Optical Transients with RAPTOR

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    Fast variability of optical objects is an interesting though poorly explored subject in modern astronomy. Real-time data processing and identification of transient celestial events in the images is very important for such study as it allows rapid follow-up with more sensitive instruments. We discuss an approach which we have developed for the RAPTOR project, a pioneering closed-loop system combining real-time transient detection with rapid follow-up. RAPTOR's data processing pipeline is able to identify and localize an optical transient within seconds after the observation. The testing we performed so far have been confirming the effectiveness of our method for the optical transient detection. The software pipeline we have developed for RAPTOR can easily be applied to the data from other experiments.Comment: 10 pages, 7 figures, to appear in SPIE proceedings vol. 484

    Loss of the Tumor Suppressor Pten Promotes Proliferation of Drosophila melanogaster Cells In Vitro and Gives Rise to Continuous Cell Lines

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    In vivo analysis of Drosophila melanogaster has enhanced our understanding of many biological processes, notably the mechanisms of heredity and development. While in vivo analysis of mutants has been a strength of the field, analyzing fly cells in culture is valuable for cell biological, biochemical and whole genome approaches in which large numbers of homogeneous cells are required. An efficient genetic method to derive Drosophila cell lines using expression of an oncogenic form of Ras (RasV12) has been developed. Mutations in tumor suppressors, which are known to cause cell hyperproliferation in vivo, could provide another method for generating Drosophila cell lines. Here we screened Drosophila tumor suppressor mutations to test if they promoted cell proliferation in vitro. We generated primary cultures and determined when patches of proliferating cells first emerged. These cells emerged on average at 37 days in wild-type cultures. Using this assay we found that a Pten mutation had a strong effect. Patches of proliferating cells appeared on average at 11 days and the cultures became confluent in about 3 weeks, which is similar to the timeframe for cultures expressing RasV12. Three Pten mutant cell lines were generated and these have now been cultured for between 250 and 630 cell doublings suggesting the life of the mutant cells is likely to be indefinite. We conclude that the use of Pten mutants is a powerful means to derive new Drosophila cell lines

    Model Cortical Association Fields Account for the Time Course and Dependence on Target Complexity of Human Contour Perception

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    Can lateral connectivity in the primary visual cortex account for the time dependence and intrinsic task difficulty of human contour detection? To answer this question, we created a synthetic image set that prevents sole reliance on either low-level visual features or high-level context for the detection of target objects. Rendered images consist of smoothly varying, globally aligned contour fragments (amoebas) distributed among groups of randomly rotated fragments (clutter). The time course and accuracy of amoeba detection by humans was measured using a two-alternative forced choice protocol with self-reported confidence and variable image presentation time (20-200 ms), followed by an image mask optimized so as to interrupt visual processing. Measured psychometric functions were well fit by sigmoidal functions with exponential time constants of 30-91 ms, depending on amoeba complexity. Key aspects of the psychophysical experiments were accounted for by a computational network model, in which simulated responses across retinotopic arrays of orientation-selective elements were modulated by cortical association fields, represented as multiplicative kernels computed from the differences in pairwise edge statistics between target and distractor images. Comparing the experimental and the computational results suggests that each iteration of the lateral interactions takes at least ms of cortical processing time. Our results provide evidence that cortical association fields between orientation selective elements in early visual areas can account for important temporal and task-dependent aspects of the psychometric curves characterizing human contour perception, with the remaining discrepancies postulated to arise from the influence of higher cortical areas
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