Revealing the spectral state transition of the Clocked Burster, GS 1826-238 with NuSTAR StrayCats

We present the long term analysis of GS 1826-238, a neutron star X-ray binary known as the "Clocked Burster", using data from NuSTAR StrayCats. StrayCats, a catalogue of NuSTAR stray light data, contains data from bright, off-axis X-ray sources that have not been focused by the NuSTAR optics. We obtained stray light observations of the source from 2014-2021, reduced and analyzed the data using nustar-gen-utils Python tools, demonstrating the transition of source from the "island" atoll state to a "banana" branch. We also present the lightcurve analysis of Type I X-Ray bursts from the Clocked Burster and show that the bursts from the banana/soft state are systematically shorter in durations than those from the island/hard state and have a higher burst fluence. From our analysis, we note an increase in mass accretion rate of the source, and a decrease in burst frequency with the transition

Observing the transient pulsations of SMC X-1 with NuSTAR

We report on NuSTAR observations of transient pulsations in the neutron star X-ray binary SMC X-1. The transition from non-pulsing to pulsing states was not accompanied by a large change in flux. Instead, both pulsing and non-pulsing states were observed in a single observation during the low-flux super-orbital state. During the high-state, we measure a pulse period of $P = 0.70117(9)\,\mathrm{s}$ at $T_{ref} = 56145\,\mathrm{MJD}$. Spectral analysis during non-pulsing and pulsing states reveals that the observations can be consistently modeled by an absorbed power law with a phenomenological cutoff resembling a Fermi-Dirac distribution, or by a partially obscured cutoff power law. The shapes of the underlying continua show little variability between epochs, while the covering fraction and column density vary between super-orbital states. The strength of pulsations also varies, leading us to infer that the absence and reemergence of pulsations are related to changing obscuration, such as by a warped accretion disk. SMC X-1 is accreting near or above its Eddington limit, reaching an unabsorbed X-ray luminosity of $L_{\rm X}({\rm 2-10~keV}) \approx 5 \times 10^{38}\, {\rm erg}\, {\rm s}^{-1}$. This suggests that SMC X-1 may be a useful local analog to ultraluminous X-ray pulsars (ULXPs), which likewise exhibit strong variability in their pulsed fractions, as well as flux variability on similar timescales. In particular, the gradual pulse turn-on which has been observed in M82 X-2 is similar to the behavior we observe in SMC X-1. Thus we propose that pulse fraction variability of ULXPs may also be due to variable obscuration.Comment: Accepted for publication in the Astrophysical Journal; 13 pages, 4 figure

Excretion and folding of plasmalemma function to accommodate alterations in guard cell volume during stomatal closure in Vicia faba L.

Stomatal movement results in large and repetitive changes in cell volume and consequently surface area. While endocytosis has been extensively studied and is thought to be a major mechanism for accommodating the volume changes as evidenced mainly by fluorescent labelling and confocal imaging, studies at the ultrastructural level in intact guard cells of stomata regulated by natural factors have never been reported. Here, it is reported that excretion and folding of the plasmalemma are critical for accommodating the volume alterations in intact guard cells in Vicia faba L. Using transmission electron microscopy in combination with laser confocal microscopy, it was observed that in fully opened stomata the plasmalemma was smooth and tightly adhered to the cell walls while a whole large vacuole appeared in the cell. In the closed stomata, besides vacuole fragmentation, endocytosis of the tonoplast rather than the plasmalemma commonly occurred. Importantly, in stomata where pore closure was induced by circadian rhythm or CO2, numerous tiny vesicles were found outside the plasmalemma and, moreover, extensive folding of the plasmalemma could also be found in some regions of the cells. Additionally, an unknown structure was found at the interface between the plasmalemma and cell walls, especially in those areas of the cell where extensive folding occurred, suggesting that plasmalemma turnover is possibly associated with an interaction between the plasmalemma and cell walls. Collectively, the results strongly indicate that excretion and folding of the plasmalemma are critical for the accommodation of the cell volume alterations during stomatal movement

Calcium-dependent release of adenosine and uridine nucleotides from A549 cells

Extracellular nucleotides play an important role in lung defense, but the release mechanism and relative abundance of different nucleotide species secreted by lung epithelia are not well defined. In this study, to minimize cell surface hydrolysis, we used a low-volume, flow-through chamber and examined adenosine and uridine nucleotide concentrations in perfusate aliquots of human lung A549 cells challenged by 50% hypotonic shock. Adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), and adenosine (Ado) were quantified in high-performance liquid chromatography (HPLC) analysis of fluorescent etheno derivatives, and uridine triphosphate (UTP) and uridine diphosphate (UDP) were measured using HPLC-coupled radioenzymatic assays. After the onset of hypotonic shock, ATP, ADP, UTP, and UDP in the perfusates increased markedly and peaked at approximately 2.5 min, followed by a gradual decay in the next 15–20 min; peak changes in Ado and AMP were relatively minor. The peak concentrations and fold increment (in parentheses) were: 34 ± 13 nM ATP (5.6), 11 ± 5 nM ADP (3.7), 3.3 ± 1.2 nM AMP (1.4), 23 ± 7 nM Ado (2.1), 21 nM UTP (>7), and 11 nM UDP (27). Nucleotide release was almost completely abolished from cells loaded with the calcium chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA). Under isotonic conditions, elevation of intracellular calcium with the calcium ionophore ionomycin (5 μM, 3 min) also released nucleotides with kinetics and relative abundance as above, albeit less robust. ADP:ATP (1:3) and UDP:UTP (1:2) ratios in perfusates from stimulated cells were markedly higher than the cytosolic ratios of these species, suggesting that a nucleotide diphosphate (NDP)-rich compartment, e.g., the secretory pathway, contributed to nucleotide release. Laser confocal microscopy experiments illustrated increased FM1-43 uptake into the plasma membrane upon hypotonic shock or ionomycin treatment, consistent with enhanced vesicular exocytosis under these conditions. In summary, our results strongly suggest that calcium-dependent exocytosis is responsible, at least in most part, for adenosine and uridine nucleotide release from A549 cells

Human cardiac pericytes are susceptible to SARS-CoV-2 infection

COVID-19 is associated with serious cardiovascular complications, with incompletely understood mechanism(s). Pericytes have key functions in supporting endothelial cells and maintaining vascular integrity. We demonstrate that human cardiac pericytes are permissive to SARS-CoV-2 infection in organotypic slice and primary cell cultures. Viral entry into pericytes is mediated by endosomal proteases, and infection leads to up-regulation of inflammatory markers, vasoactive mediators, and nuclear factor kappa-B-dependent cell death. Furthermore, we present evidence of cardiac pericyte infection in COVID-19 myocarditis patients. These data demonstrate that human cardiac pericytes are susceptible to SARS-CoV-2 infection and suggest a role for pericyte infection in COVID-19

MAXI and NuSTAR observations of the faint X-ray transient MAXI J1848-015 in the GLIMPSE-C01 Cluster

We present the results of MAXI monitoring and two NuSTAR observations of the recently discovered faint X-ray transient MAXI J1848-015. Analysis of the MAXI light-curve shows that the source underwent a rapid flux increase beginning on 2020 December 20, followed by a rapid decrease in flux after only $\sim5$ days. NuSTAR observations reveal that the source transitioned from a bright soft state with unabsorbed, bolometric ($0.1$-$100$ keV) flux $F=6.9 \pm 0.1 \times 10^{-10}\,\mathrm{erg\,cm^{-2}\,s^{-1}}$, to a low hard state with flux $F=2.85 \pm 0.04 \times 10^{-10}\,\mathrm{erg\,cm^{-2}\,s^{-1}}$. Given a distance of $3.3$ kpc, inferred via association of the source with the GLIMPSE-C01 cluster, these fluxes correspond to an Eddington fraction of order $10^{-3}$ for an accreting neutron star of mass $M=1.4M_\odot$, or even lower for a more massive accretor. However, the source spectra exhibit strong relativistic reflection features, indicating the presence of an accretion disk which extends close to the accretor, for which we measure a high spin, $a=0.967\pm0.013$. In addition to a change in flux and spectral shape, we find evidence for other changes between the soft and hard states, including moderate disk truncation with the inner disk radius increasing from $R_\mathrm{in}\approx3\,R_\mathrm{g}$ to $R_\mathrm{in}\approx8\,R_\mathrm{g}$, narrow Fe emission whose centroid decreases from $6.8\pm0.1$ keV to $6.3 \pm 0.1$ keV, and an increase in low-frequency ($10^{-3}$-$10^{-1}$ Hz) variability. Due to the high spin we conclude that the source is likely to be a black hole rather than a neutron star, and we discuss physical interpretations of the low apparent luminosity as well as the narrow Fe emission.Comment: 19 pages, 9 figures, 3 tables. Accepted for publication in Ap

Impact Factor: outdated artefact or stepping-stone to journal certification?

A review of Garfield's journal impact factor and its specific implementation as the Thomson Reuters Impact Factor reveals several weaknesses in this commonly-used indicator of journal standing. Key limitations include the mismatch between citing and cited documents, the deceptive display of three decimals that belies the real precision, and the absence of confidence intervals. These are minor issues that are easily amended and should be corrected, but more substantive improvements are needed. There are indications that the scientific community seeks and needs better certification of journal procedures to improve the quality of published science. Comprehensive certification of editorial and review procedures could help ensure adequate procedures to detect duplicate and fraudulent submissions.Comment: 25 pages, 12 figures, 6 table

Observing the Transient Pulsations of SMC X-1 with NuSTAR

We report on Nuclear Spectroscopic Telescope Array observations of transient pulsations in the neutron star X-ray binary SMC X-1. The transition from nonpulsing to pulsing states was not accompanied by a large change in flux. Instead, both pulsing and nonpulsing states were observed in a single observation during the low-flux super-orbital state. During the high state, we measure a pulse period of P = 0.70117(9) s at T_(ref) = 56145 MJD. Spectral analysis during nonpulsing and pulsing states reveals that the observations can be consistently modeled by an absorbed power law with a phenomenological cutoff resembling a Fermi–Dirac distribution, or by a partially obscured cutoff power law. The shapes of the underlying continua show little variability between epochs, while the covering fraction and column density vary between super-orbital states. The strength of pulsations also varies, leading us to infer that the absence and reemergence of pulsations are related to changing obscuration, such as by a warped accretion disk. SMC X-1 is accreting near or above its Eddington limit, reaching an unabsorbed X-ray luminosity of L_X(2–10 keV) ≈ 5 × 10^(38) erg s^(−1). This suggests that SMC X-1 may be a useful local analog to ultraluminous X-ray pulsars (ULXPs), which likewise exhibit strong variability in their pulsed fractions, as well as flux variability on similar timescales. In particular, the gradual pulse turn-on, which has been observed in M82 X-2, is similar to the behavior we observe in SMC X-1. Thus we propose that pulse fraction variability of ULXPs may also be due to variable obscuration

Orbital Decay in M82 X-2

© 2022. The Author(s). Published by the American Astronomical Society. This is an open access article distributed under the Creative Commons Attribution License, to view a copy of the license, https://creativecommons.org/licenses/by/4.0/M82 X-2 is the first pulsating ultraluminous X-ray source discovered. The luminosity of these extreme pulsars, if isotropic, implies an extreme mass transfer rate. An alternative is to assume a much lower mass transfer rate, but with an apparent luminosity boosted by geometrical beaming. Only an independent measurement of the mass transfer rate can help discriminate between these two scenarios. In this paper, we follow the orbit of the neutron star for 7 yr, measure the decay of the orbit ( Ṗorb/Porb≈−8·10−6yr−1 ), and argue that this orbital decay is driven by extreme mass transfer of more than 150 times the mass transfer limit set by the Eddington luminosity. If this is true, the mass available to the accretor is more than enough to justify its luminosity, with no need for beaming. This also strongly favors models where the accretor is a highly magnetized neutron star.Peer reviewe

The Role of Climate Variability in the Spread of Malaria in Bangladeshi Highlands

Malaria is a major public health problem in Bangladesh, frequently occurring as epidemics since the 1990s. Many factors affect increases in malaria cases, including changes in land use, drug resistance, malaria control programs, socioeconomic issues, and climatic factors. No study has examined the relationship between malaria epidemics and climatic factors in Bangladesh. Here, we investigate the relationship between climatic parameters [rainfall, temperature, humidity, sea surface temperature (SST), El Niño-Southern Oscillation (ENSO), the normalized difference vegetation index (NDVI)], and malaria cases over the last 20 years in the malaria endemic district of Chittagong Hill Tracts (CHT)