Сравнительная оценка экспрессии молекул главного комплекса гистосовместимости в тканях пародонта и периферической крови больных генерализованным пародонтитом

Проведено порівняльні клініко-іммунологічні аналізи стану адгезивних молекул HLA-A, B, C і HLA-DR головного комплексу гістосумісності на місцевому рівні - в тканинах пародонта і периферичної крові хворих на ГП і відповідних антигенів моноклональних антитіл T і В-лімфоцитів. Не виявлено прямого кореляційного зв’язку між клінічним проявом запалення пародонту і загальносоматичним імунним статусом, що розкриває механізми локальних Т-клітинних характеристик імунних змін і зумовлює корекцію місцевої терапії.A comparative clinical and immunological analysis of adhesion molecules HLA-A, B, C and HLA-DR major histocompatibility complex at the local level - in periodontal tissues and peripheral blood of patients with SE and related antigen antibody monoklialnyh T and B lymphocytes. There were no direct connection between korellyatsionnoy clinical manifestation of periodontal inflammation and the immune status of the somatic, that reveals the mechanisms of local T-cell characteristics of the immune changes and determines the correction of local therapy

Initial impacts of global risk mitigation measures taken during the combatting of the COVID-19 pandemic

This paper presents an analysis of risk mitigation measures taken by countries around the world facing the current COVID-19 outbreak. In light of the current pandemic the authors collated and clustered (using harmonised terminology) the risk mitigation measures taken around the globe in the combat to contain, and since March 11 2020, to limiting the spread of the SARS-CoV-2 virus known to cause the Coronavirus disease 2019 (COVID-19). This overview gathers lessons learnt, provides an update on the current knowledge for authorities, sectors and first responders on the effectiveness and may allow enhanced prevention, preparedness and response for future outbreaks. Various measures such as mobility restrictions, physical distancing, hygienic measures, socio economic restrictions, communication and international support mechanisms have been clustered and are reviewed in terms of the nature of the actions taken and their qualitative early-perceived impact. At the time of writing, it is still too premature to express the quantitative effectiveness of each risk mitigation cluster, but it seems that the best mitigation results are reported when applying a combination of voluntary and enforceable measures.JRC.E.7-Knowledge for Security and Migratio

Security and defence research in the European Union: a landscape review

This landscape report describes the state of play of the European Union’s policies and activities in security and defence and the EU-funded research aimed at supporting them, with an exclusive focus on intentional harm. It is organised around several thematic building blocks under the umbrella of the three core priorities defined in the European agenda on security. The report reviews the current main risks and threats but also those that may emerge within the next 5 years, the policy and operational means developed to combat them, the main active stakeholders and the EU legislation in force. In this context, a short history of EU research on security and defence is presented, followed by an inventory of relevant research and development projects funded under the Horizon 2020 framework programme during the period 2014-2018. The specific contributions of the Joint Research Centre to security research are also highlighted. Finally, future avenues for security and defence research and development are discussed. Please note that the executive summary of this landscape report has been published simultaneously as a companion document.JRC.E.7-Knowledge for Security and Migratio

How is the EU CBRN CoE contributing to fighting the COVID-19 pandemic?

How is the EU CBRN CoE contributing to fighting the COVID-19 pandemic?JRC.A.7-Euratom Coordinatio

ToF-SIMS parallel imaging MS/MS of lipid species in thin tissue sections

Unambiguous identification of detected species is essential in complex biomedical samples. To date, there are not many mass spectrometry imaging techniques that can provide both high spatial resolution and identification capabilities. A new and patented imaging tandem mass spectrometer, exploiting the unique characteristics of the nanoTOF II (Physical Electronics, USA) TOF-SIMS TRIFT instrument, was developed to address this. Tandem mass spectrometry is based on the selection of precursor ions from the full secondary ion spectrum (MS1), followed by energetic activation and fragmentation, and collection of the fragment ions to obtain a tandem MS spectrum (MS2). The PHI NanoTOF II mass spectrometer is equipped with a high-energy collision induced dissociation (CID) fragmentation cell as well as a second time-of-flight analyzer developed for simultaneous ToF-SIMS and tandem MS imaging experiments. We describe here the results of a ToF-SIMS imaging experiment on a thin tissue section of an infected zebrafish as a model organism for tuberculosis. The focus is on the obtained ion distribution plot of a fatty acid as well as its identification by tandem mass spectrometry

Identification and High-Resolution Imaging of alpha-Tocopherol from Human Cells to Whole Animals by TOF-SIMS Tandem Mass Spectrometry

A unique method for identification of biomolecular components in different biological specimens, while preserving the capability for high speed 2D and 3D molecular imaging, is employed to investigate cellular response to oxidative stress. The employed method enables observing the distribution of the antioxidant α-tocopherol and other molecules in cellular structures via time-of-flight secondary ion mass spectrometry (TOF-SIMS (MS1)) imaging in parallel with tandem mass spectrometry (MS2) imaging, collected simultaneously. The described method is employed to examine a network formed by neuronal cells differentiated from human induced pluripotent stem cells (iPSCs), a model for investigating human neurons in vitro. The antioxidant α-tocopherol is identified in situ within different cellular layers utilizing a 3D TOF-SIMS tandem MS imaging analysis. As oxidative stress also plays an important role in mediating inflammation, the study was expanded to whole body tissue sections of M. marinum-infected zebrafish, a model organism for tuberculosis. The TOF-SIMS tandem MS imaging results reveal an increased presence of α-tocopherol in response to the pathogen

Mass Spectrometry Imaging of Drug Related Crystal-Like Structures in Formalin-Fixed Frozen and Paraffin-Embedded Rabbit Kidney Tissue Sections

A multimodal mass spectrometry imaging (MSI) based approach was used to characterize the molecular content of crystal-like structures in a frozen and paraffin embedded piece of a formalin-fixed rabbit kidney. Matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) imaging and desorption electrospray ionization (DESI) mass spectrometry imaging were combined to analyze the frozen and paraffin embedded sample without further preparation steps to remove the paraffin. The investigated rabbit kidney was part of a study on a drug compound in development, in which severe renal toxicity was observed in dosed rabbits. Histological examination of the kidney showed tubular degeneration with precipitation of crystal-like structures in the cortex, which were assumed to cause the renal toxicity. The MS imaging approach was used to find out whether the crystal-like structures were composed of the drug compound, metabolites, or an endogenous compound as a reaction to the drug administration. The generated MALDI-MSI data were analyzed using principal component analysis. In combination with the MS/MS results, this way of data processing demonstrates that the crystal structures were mainly composed of metabolites and relatively little parent drug. [Figure: see text