Draft Genome Sequence for Desulfovibrio africanus Strain PCS.

Desulfovibrio africanus strain PCS is an anaerobic sulfate-reducing bacterium (SRB) isolated from sediment from Paleta Creek, San Diego, CA. Strain PCS is capable of reducing metals such as Fe(III) and Cr(VI), has a cell cycle, and is predicted to produce methylmercury. We present the D. africanus PCS genome sequence

Mouse Model of Devil Facial Tumour Disease Establishes That an Effective Immune Response Can be Generated Against the Cancer Cells

The largest carnivorous marsupial in Australia, the Tasmanian devil (Sarcophilus harrisii) is facing extinction in the wild due to a transmissible cancer known as Devil Facial Tumour Disease (DFTD). DFTD is a clonal cell line transmitted from host to host with 100% mortality and no known immunity. While it was first considered that low genetic diversity of the population of devils enabled the allograft transmission of DFTD recent evidence reveals that genetically diverse animals succumb to the disease. The lack of an immune response against the DFTD tumor cells may be due to a lack of immunogenicity of the tumor cells. This could facilitate transmission between devils. To test immunogenicity, mice were injected with viable DFTD cells and anti-DFTD immune responses analyzed. A range of antibody isotypes against DFTD cells was detected, indicating that as DFTD cells can induce an immune response they are immunogenic. This was supported by cytokine production, when splenocytes from mice injected with DFTD cells were cultured in vitro with DFTD cells and the supernatant analyzed. There was a significant production of IFN-γ and TNF-α following the first injection with DFTD cells and a significant production of IL-6 and IL-10 following the second injection. Splenocytes from naïve or immunized mice killed DFTD cells in in vitro cytotoxicity assays. Thus they are also targets for immunological destruction. We conclude that as an immune response can be generated against DFTD cells they would be suitable targets for a vaccine

Design of University Small-Scale Dairy Processing Facility

This project was assigned to research the feasibility and value of implementing a dairy processing facility on the campus of the University of Nebraska-Lincoln. The facility would process milk produced from cows under the same roof and will serve as an educational experience for Nebraska dairy farmers, UNL students, and K-12 students in the Lincoln-Lancaster County area. If the project is successful and replicated across the state, this facility could have a significant impact on the reduction of milk transportation costs in the Nebraska dairy industry. The project began with researching milk processing methods and steps from production to consumption. Shortly after this step, information on milk consumption patterns was collected from UNL Dining Services to determine demand on campus. Every unit operation requires certain equipment to effectively ensure the safety and quality of the final product, and mass balances from UNL milk consumption data were used to size equipment and storage capacity. Engineering firms were then consulted to gather information on equipment specifications and prices. Equipment costs and operating costs (estimated with the help of Dr. Howell and other university dairy operations) were entered into a Monte Carlo simulation to analyze return on investment and a breakeven point. The results from the costs section showed that the fixed costs (equipment and engineering) for the milk processing would be about $1.2 million. The Monte Carlo simulation showed that the project would not turn a profit for 10-12 years, and approximately 2.25 million gallons of milk would need to be processed and sold to recover initial costs. Overall, the project successfully displays data that can be interpreted by the client to decide whether to move forward with the project and the appropriate scale for the project at UNL

In-beam gamma-ray spectroscopy of 35Mg and 33Na

Excited states in the very neutron-rich nuclei 35Mg and 33Na were populated in the fragmentation of a 38Si projectile beam on a Be target at 83 MeV/u beam energy. We report on the first observation of gamma-ray transitions in 35Mg, the odd-N neighbor of 34Mg and 36Mg, which are known to be part of the "Island of Inversion" around N = 20. The results are discussed in the framework of large- scale shell-model calculations. For the A = 3Z nucleus 33Na, a new gamma-ray transition was observed that is suggested to complete the gamma-ray cascade 7/2+ --> 5/2+ --> 3/2+ gs connecting three neutron 2p-2h intruder states that are predicted to form a close-to-ideal K = 3/2 rotational band in the strong-coupling limit.Comment: Accepted for publication Phys. Rev. C. March 16, 2011: Replaced figures 3 and 5. We thank Alfredo Poves for pointing out a problem with the two figure

Interactive scenario design: The value of flowcharts and schemas in developing scenario-based lessons for online and flexible learning contexts

A web-delivered problem-based scenario was designed for use in a distance education professional development workshop for academics, and also as a stand-alone module. Early scenario design and development was assisted with flowcharts and iterative table-based schemas, which formalised and documented the process before authoring in the e-tool, SBL Interactive. It is well established that such planning techniques can scaffold the course development process. While the flowcharts and schemas described in this paper are designed for use with this tool, the methodology described for their use as planning tools applies generally to the design of interactive electronically-delivered problem-based scenarios. They also allow scenario descriptions and content to be archived and shared in an easily accessible form. The paper illustrates the basic principle that, when designing a course, there are many choices about what, when, where, and how to teach. It provides an account of how conventional design techniques can be used alongside new e-tools to systematically select and optimise the most appropriate instructional blend for a particular learning context

Spectroscopy of the odd-odd fp-shell nucleus 52Sc from secondary fragmentation

The odd-odd fp-shell nucleus 52Sc was investigated using in-beam gamma-ray spectroscopy following secondary fragmentation of a 55V and 57Cr cocktail beam. Aside from the known gamma-ray transition at 674(5)keV, a new decay at E_gamma=212(3) keV was observed. It is attributed to the depopulation of a low-lying excited level. This new state is discussed in the framework of shell-model calculations with the GXPF1, GXPF1A, and KB3G effective interactions. These calculations are found to be fairly robust for the low-lying level scheme of 52Sc irrespective of the choice of the effective interaction. In addition, the frequency of spin values predicted by the shell model is successfully modeled by a spin distribution formulated in a statistical approach with an empirical, energy-independent spin-cutoff parameter.Comment: accepted for publication in PR