Desenvolvimento pós-embrionário de Clibanarius antillensis Stimpson, 1859 (Crustacea, Diogenidae), em laboratório

Este trabalho foi realizado com a finalidade de descrever a anatomia externa das larvas de Clibanarius antillensis STIMPSON, 1859 criadas em laboratório. Os estádio de zoea e megalopa foram descritos. Fêmeas ovígeras foram coletadas em praias de S. Sebastião, São Paulo, Brasil. Os animais foram mantidos até a eclosão no laboratório em água do mar filtrada (salinidade 35 °/oo). As larvas foram criadas individualmente e alimentadas com nauplius de Artemia salina e a água foi mudada diariamente. Quatro ou cinco estádios de zoea e megalopa foram obtidos e o período de desenvolvimento foi completado em 39 dias (valor médio) à 18,8°C e 29 dias à 22°C. Comparações anatômicas foram feitas com larvas de outras espécies brasileiras particularmente com as do mesmo gênero. Observou- se estreita correlação entre C.antillensis e C. vittatus (Bosc, 1802)

Upogebia paraffinis Williams (Decapoda, Upogebiidae): biologia e nova ocorrência para o litoral do Estado do Paraná (Brasil)

Upogebia paraffinis Williams, 1993 (Decapoda: Thalassinidea: Upogebiidae) capturada em praia próxima a desembocadura do rio Boguaçu, localizada no município de Pontal do Paraná, Estado do Paraná, Brasil (25° 33 00 S 48° 23 46W). Com o presente trabalho, sua distribuição latitudinal fica ampliada desde o Estado do Ceará até o Estado do Paraná, Brasil. Esta espécie habita galerias construídas em praias areno-lodosas, próximo a regiões de mangue, com profundidades de até 30cm, cerca de 3 m acima da linha da maré baixa. ABSTRACT Upogebia paraffinis Williams, 1993 (Decapoda: Thalassinidea: Upogebiidae) was captured at a beach near Boguaçu river, at Pontal do Paraná, Paraná State, Brazil (25° 33 00 S 48° 23 46W). With the present register its latitudinal distribution is amplified from Ceará to Paraná State coastline Brazil. This species live in galleries constructed on muddy beaches near the mangrove regions. The burrows were about 30cm deep with openings until 3m above low tide mark. RÉSUMÉ Nouveau régistre de Upogebia paraffinis Williams, 1993 (Decapoda: Thalassinidea: Upogebiidae) dans le littoral du Paraná, Brésil (25° 33 00 S 48° 23 46W). La distribution latitudinale actuellement est sur le littoral de le Ceará jusquau Paraná. Upogebia paraffinis a été prélevée en galleries construites en plages bourbeux proches à des mangroves. Les tubes de la gallerie sont construits à profundeur denviron 30cm, avec des ouvertures 3m sur de la ligne de marée minimale

Determinação seletiva de tributilestanho na presença de Sn(IV) em amostras ambientais usando HG-ICP OES e Saccharomyces cerevisiae como material sorvente

A method for selective determination of tributyltin in the presence of Sn(IV) by combining hydride generation inductively coupled plasma optical emission spectrometry (HG-ICP OES) and solid phase extraction (SPE) using baker's yeast is proposed. The procedure is based on selective retention of TBT by the yeast at pH = 6. Detection limit of 1.9 µg L-1 and quantification limit of 6.3 µg L-1 were obtained. TBT and San(IV) were determined in the range of 0-25 µg L-1 and the proposed approach was applied to analyze river water, sea water and biological extracts, with recoveries of 114, 101 and 86%, respectively

Desenvolvimento larval de Macrobrachium birai Lobão, Melo & Fernandes (Crustacea, Decapoda, Caridea, Palaemonidae) em laboratório

Macrobrachium birai Lobão, Melo & Fernandes, 1986, espécie de camarão de água doce do Brasil, foi capturada no Rio Branco, Cananéia, Estado de São Paulo, Brasil. No início do desenvolvimento embrionário os ovos têm cor verde oliva e tamanho médio de 0,60 x 0,43 mm. As larvas foram criadas, individualmente, a temperatura média de 25ºC. O desenvolvimento larval de Macrobrachium birai é do tipo prolongado, constituído de 15 estágios morfológicos de zoea e o decapodito. A metamorfose foi atingida em 144 dias de cultivo, através de 44 ecdises. As mudas ocorreram em intervalos de dois a oito dias e em média com 3,3 dias. As larvas de M. birai possuem algumas características morfológicas que podem diferenciá-las das larvas de M. olfersii (Wiegmann, 1836).Macrobrachium birai Lobão, Melo & Fernandes, 1986, a native Brazilian species of freshwater shrimp, was captured along the coast of Rio Branco, Cananéia, state of São Paulo, Brazil. The eggs in early development were green and the average size was 0.60 x 0.43 mm. The larvae were reared in laboratory at average temperature of 25ºC. Macrobrachium birai has a prolonged larval development, with 15 morphological zoeal stages and the decapodito. The metamorphose was obtained in 144 days, through about 44 ecdysis. The molts occurred from two to eight days, in average of 3.3 days. The larvae of the M. birai present some morphological characteristics distinct from that of M. olfersii (Wiegmann, 1836).Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Surface morphology of Diplodon expansus (Kuster, 1856; Mollusca, Bivalvia, Hyriidae) gill filaments after exposure to environmentally relevant concentrations of atrazine herbicide

Brazilian endemic species Diplodon expansus (Kuster, 1856) is found in freshwater bodies in the country's southeast, in large anthropogenic influence regions especially with an extensive agriculture emphasis. One of the main pesticides used in the species occurrence region is the atrazine herbicide, which has a great contamination potential in the aquatic environment. Therefore, several studies into its toxicity in aquatic systems have been developed. However, the tested concentrations are usually very high and rarely found in the environment and the short-term exposure responses in other aquatic organisms such as native bivalves are still scarce. Thus, this study sought to consider the potential effects of environmentally realistic concentrations of atrazine herbicide on the surface morphology of gill filaments of the bivalve D. expansus under laboratory-controlled conditions after short-term exposure. None of the animals died before the end of the experiment. The main alterations were observed on the frontal surface of filaments, which include mucus accumulation, cilia loss, and disruption. Mucus increased secretion and accumulation in the frontal filaments region preceded as a protective mechanism. Cilia loss and disruption on the frontal surface of the gill filament indicated that ciliated frontal cells were more sensitive to atrazine exposure and these alterations may cause gills functional damages, compromising the uptake of food particles and respiration. Therefore, higher sublethal concentrations of atrazine may compromise the survival and consequently the population of D. expansus in agriculture areas after a longer period of continuous exposure. Microsc. Res. Tech. 75:807813, 2012. (C) 2011 Wiley Periodicals, Inc.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Morphological and Histochemical Characterization of Gill Filaments of the Brazilian Endemic Bivalve Diplodon expansus (Kuster, 1856) (Mollusca, Bivalvia, Hyriidae)

This study presents the morphological description and histochemical characterization of gill filaments of the Brazilian endemic bivalve Diplodon expansus, aiming to broaden the morphological knowledge of this species and establish the structure of the gills that will serve as control in histopathological studies applied to biomonitoring. The gill filaments are divided into three zones: frontal, intermediate, and abfrontal. In the center of the filament, haemocytes circulate through the haemolymph vessel, which is internally lined by endothelium. The frontal surface of the filament is covered with cilia, the lateral surface exhibits aquifer ducts, and the abfrontal surface presents ciliated and nonciliated cells. The epithelium of the filaments is composed of ciliated cells, nonciliated absorptive cells, and mucocytes. The support of the filaments is made by two specialized structures called skeletal rod and skeletal loop. Based on the obtained information, the gill filaments of the studied species present some peculiar characteristics that are not yet reported in detail in the literature such as the simultaneous presence of skeletal rod and skeletal loop. on the other hand, the general constitution of the filament is similar to that described for both marine and limnic bivalves and seems to be suitable for ecotoxicological studies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Use of the freshwater crab Trichodactylus fluviatilis to biomonitoring Al and Mn contamination in river water.

Determinations of Al and Mn concentrations in the tissues (gills, hepatopancreas and muscle) of the freshwater crab Trichodactylus fluviatilis and water samples, both collected from sites on tributaries of the Corumbatai River (São Paulo, Brazil) were performed. The Bioaccumulation Factor (BAF), calculated for different sites with respect to the water concentration, ranged from 173-555 for Al and from 636 - 921 for Mn. Dissolved concentrations of Al and Mn in water samples (collected in different sites) were related to the accumulation of these metals in crabs, suggesting that T. fluviatilis is good biomonitor for Al and Mn pollution in aquatic ecosystem

Zoeal morphology of Pachygrapsus transversus (Gibbes) (Decapoda, Grapsidae) reared in the laboratory

Ovigerous females of Pachygrapsus transversus (Gibbes, 1850) were collected on the Praia Dura and Saco da Ribeira beaches, Ubatuba, São Paulo, Brazil. Larvae were individually reared in a climatic room at 25ºC temperature, salinities of 28, 32 and 35‰ and under natural photoperiod conditions. The best rearing results were observed at 35%o salinity. Seven zoeal instars were observed, drawing and described in detail. The data are compared with those obtained for P. gracilis (Saussure, 1858)