Detailed requirements document for the radiant heat transfer facility post-test data reduction program

The requirements and functional specifications for a program to process test data obtained by the Radiant Heat Data Acquisition System are defined

Assignment of the lattice modes in TCNQ0 single crystals

A complete assignment of the six librational modes of the TCNQ0 crystal has been made employing both polarized Raman spectroscopic measurements and lattice dynamical calculations. Agreement between theory and experiment is good and modes at 40.5, 74.5, and 96 cm-1 are assigned as Ag active and those at 63, 75.5, and 104 cm-1 are Bg active. The lattice modes are found to be clearly separated from the molecular modes. The study emphasizes the importance of crystal optics and quality in the measurement of the polarized Raman spectra of biaxial crystals. The Journal of Chemical Physics is copyrighted by The American Institute of Physics

Neural correlates of up-regulating positive emotions in fMRI and their link to affect in daily life

Emotion regulation is typically used to down-regulate negative or up-regulate positive emotions. While there is considerable evidence for the neural correlates of the former, less is known about the neural correlates of the latter—and how they are associated with emotion regulation and affect in daily life. Functional magnetic resonance imaging (fMRI) data were acquired from 63 healthy young participants (22 ± 1.6 years, 30 female), while they up-regulated their emotions to positive and neutral images or passively watched them. The same participants’ daily affect and emotion regulation behavior was measured using experience sampling over 10 days. Focusing on the ventral striatum (VS), previously associated with positive affective processing, we found increased activation during the up-regulation to both positive and neutral images. VS activation for the former positively correlated with between- and within-person differences in self-reported affective valence during fMRI but was not significantly associated with up-regulation in daily life. However, participants with lower daily affect showed a stronger association between changes in affect and activation in emotion-related (medial frontal and subcortical) regions—including the VS. These results support the involvement of the VS in up-regulating positive emotions and suggest a neurobehavioral link between emotion-related brain activation and daily affect

CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins

SummaryBefore transmitter-filled synaptic vesicles can fuse with the plasma membrane upon stimulation they have to be primed to fusion competence. The regulation of this priming process controls the strength and plasticity of synaptic transmission between neurons, which in turn determines many complex brain functions. We show that CAPS-1 and CAPS-2 are essential components of the synaptic vesicle priming machinery. CAPS-deficient neurons contain no or very few fusion competent synaptic vesicles, which causes a selective impairment of fast phasic transmitter release. Increases in the intracellular Ca2+ levels can transiently revert this defect. Our findings demonstrate that CAPS proteins generate and maintain a highly fusion competent synaptic vesicle pool that supports phasic Ca2+ triggered release of transmitters

Harm perceptions of e-cigarettes among smokers with and without mental health conditions in England: A cross-sectional population survey

INTRODUCTION: E-cigarettes (ECs) may benefit smokers with mental health conditions who are more likely to smoke, and smoke more heavily, than those without mental health conditions. This could be undermined if harm misperceptions in this group are high as is the case in the general population. This study aimed to assess EC harm perceptions relative to cigarettes as a function of mental health status and a variety of characteristics. METHODS: Data were collected from 6,531 current smokers in 2016/17 in household surveys of representative samples of adults. The associations of mental health status (self-reported mental health condition and past year treatment), smoking and EC use characteristics, and characteristics relating to use of potential information sources with harm perceptions of ECs relative to cigarettes (measured by correct response 'less harmful' vs wrong responses 'more harmful', 'equally harmful', 'don't know') were analysed with logistic regression. RESULTS: A similar proportion of smokers without mental health conditions (61.5%, 95% CI 60.1-62.9) and with mental health conditions (both with [61.3%, 95% CI 58.7-63.8] and without past year treatment [61.5%, 95% CI 58.1-64.7] held inaccurate EC harm perceptions (all P>0.05). Being female, non-white, aged 25-34 compared with 16-24, from lower social grades (C2, D and E), not having post-16 qualifications, no EC experience, a daily smoker, unmotivated to quit <1 month, non-internet user and non-broadsheet reader were all associated with more inaccurate harm perceptions (all P<0.05). CONCLUSIONS: The majority of smokers in England have inaccurate harm perceptions of ECs regardless of mental health status

Prescribed Fire Impacts to Amphibians and Reptiles in Shelterwood-harvested Oak-dominated Forests

As part of a larger study examining the role of prescribed fire in regenerating upland oaks (Quercus spp.), seasonal prescribed burns (winter, spring, summer, and unburned control) were applied to first-stage shelterwood-harvested stands on Horsepen Wildlife Management Area in the Virginia Piedmont in 1995. Because fire impacts are poorly documented for herpetofaunal communities, we surveyed these stands in 1996 capturing 133 individuals of ten species during over 12,720 pitfall trapnights. We found no significant differences in relative abundance of Eastern Red-backed Salamanders (Plethodon cinereus) (P = 0.26), American Toads (Bufo americanus) (P = 0.93), or all amphibians combined (P = 0.25) among unburned shelterwood stands and those treated with winter, spring, or summer burns. Three species of reptiles (Northern Fence Lizard [Sceloporus undulatus], Ground Skink [Scincella lateralis], and Southeastern Five-lined Skink [Eumeces inexpectatus]) combined were captured more frequently in burned versus unburned stands (P = 0.02). Based on a stepwise multiple regression model, Eastern Red-backed Salamander captures were more strongly influenced by landscape variables (P = 0.0320), including distance to permanent water and mesic (i.e., eastern-northern) aspects, than by fire treatments (P = 0.26). Similar landscape models were not significant (P \u3c 0.05) for toads or reptiles. Based on these results, prescribed fire may not be detrimental to herpetofaunal communities in oak dominated forests in the Virginia Piedmont

Photoswitchable diacylglycerols enable optical control of protein kinase C.

Increased levels of the second messenger lipid diacylglycerol (DAG) induce downstream signaling events including the translocation of C1-domain-containing proteins toward the plasma membrane. Here, we introduce three light-sensitive DAGs, termed PhoDAGs, which feature a photoswitchable acyl chain. The PhoDAGs are inactive in the dark and promote the translocation of proteins that feature C1 domains toward the plasma membrane upon a flash of UV-A light. This effect is quickly reversed after the termination of photostimulation or by irradiation with blue light, permitting the generation of oscillation patterns. Both protein kinase C and Munc13 can thus be put under optical control. PhoDAGs control vesicle release in excitable cells, such as mouse pancreatic islets and hippocampal neurons, and modulate synaptic transmission in Caenorhabditis elegans. As such, the PhoDAGs afford an unprecedented degree of spatiotemporal control and are broadly applicable tools to study DAG signaling

Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP-25

AbstractProtein kinase A (PKA) is a key regulator of neurosecretion, but the molecular targets remain elusive. We combined pharmacological manipulations of kinase and phosphatase activities with mutational studies on the exocytotic machinery driving fusion of catecholamine-containing vesicles from chromaffin cells. We found that constitutive PKA activity was necessary to maintain a large number of vesicles in the release-ready, so-called primed, state, whereas calcineurin (protein phosphatase 2B) activity antagonized this effect. Overexpression of the SNARE protein SNAP-25a mutated in a PKA phosphorylation site (Thr-138) eliminated the effect of PKA inhibitors on the vesicle priming process. Another, unidentified, PKA target regulated the relative size of two different primed vesicle pools that are distinguished by their release kinetics. Overexpression of the SNAP-25b isoform increased the size of both primed vesicle pools by a factor of two, and mutations in the conserved Thr-138 site had similar effects as in the a isoform