Chandra X-ray observation of the HII region Gum 31 in the Carina Nebula complex

(abridged) We used the Chandra observatory to perform a deep (70 ksec) X-ray observation of the Gum 31 region and detected 679 X-ray point sources. This extends and complements the X-ray survey of the central Carina nebula regions performed in the Chandra Carina Complex Project. Using deep near-infrared images from our recent VISTA survey of the Carina nebula complex, our Spitzer point-source catalog, and optical archive data, we identify counterparts for 75% of these X-ray sources. Their spatial distribution shows two major concentrations, the central cluster NGC 3324 and a partly embedded cluster in the southern rim of the HII region, but majority of X-ray sources constitute a rather homogeneously distributed population of young stars. Our color-magnitude diagram analysis suggests ages of ~1-2 Myr for the two clusters, whereas the distributed population shows a wider age range up to ~10 Myr. We also identify previously unknown companions to two of the three O-type members of NGC 3324 and detect diffuse X-ray emission in the region. Our results suggests that the observed region contains about 4000 young stars in total. The distributed population is probably part of the widely distributed population of ~ 1-10 Myr old stars, that was identified in the CCCP area. This implies that the global stellar configuration of the Carina nebula complex is a very extended stellar association, in which the (optically prominent) clusters contain only a minority of the stellar population.Comment: Accepted for publication in Astronomy & Astrophysics. A high quality preprint is available at http://www.usm.uni-muenchen.de/people/preibisch/publications.htm

Structure of the Cytoplasmic Loop between Putative Helices II and III of the Mannitol Permease of Escherichia coli: A Tryptophan and 5-Fluorotryptophan Spectroscopy Study

In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EIImtl, are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy. The four positions, 97, 114, 126, and 133, are located in a region shown by recent studies to be involved in the mannitol translocation process. To spectroscopically distinguish between the Trp positions in each subunit of dimeric EIImtl, 5-FTrp was biosynthetically incorporated because of its much simpler photophysics compared to those of Trp. The steady-state and time-resolved fluorescence methodologies used point out that all four positions are in structured environments, both in the absence and in the presence of a saturating concentration of mannitol. The fluorescence decay of all 5-FTrp-containing mutants was highly homogeneous, suggesting similar microenvironments for both probes per dimer. However, Stern-Volmer quenching experiments using potassium iodide indicate different solvent accessibilities for the two probes at positions 97 and 133. A 5 Å two-dimensional (2D) projection map of the membrane-embedded IICmtl dimer showing 2-fold symmetry is available. The results of this work are in better agreement with a 7 Å projection map from a single 2D crystal on which no symmetry was imposed.

X-ray emission from the double-binary OB-star system QZ Car (HD 93206)

X-ray observations of the double-binary OB-star system QZ Car (HD 93206) obtained with the Chandra X-ray Observatory over a period of roughly 2 years are presented. The orbit of systems A (O9.7 I+b2 v, PA = 21 d) and B (O8 III+o9 v, PB = 6 d) are reasonably well sampled by the observations, allowing the origin of the X-ray emission to be examined in detail. The X-ray spectra can be well fitted by an attenuated three temperature thermal plasma model, characterised by cool, moderate, and hot plasma components at kT ~ 0.2, 0.7, and 2 keV, respectively, and a circumstellar absorption of ~ 0.2 x 10^22 cm-2. Although the hot plasma component could be indicating the presence of wind-wind collision shocks in the system, the model fluxes calculated from spectral fits, with an average value of ~ 7 x 10^-13 erg s-1 cm-2, do not show a clear correlation with the orbits of the two constituent binaries. A semi-analytical model of QZ Car reveals that a stable momentum balance may not be established in either system A or B. Yet, despite this, system B is expected to produce an observed X-ray flux well in excess of the observations. If one considers the wind of the O8 III star to be disrupted by mass transfer the model and observations are in far better agreement, which lends support to the previous suggestion of mass-transfer in the O8 III + o9 v binary. We conclude that the X-ray emission from QZ Car can be reasonably well accounted for by a combination of contributions mainly from the single stars and the mutual wind-wind collision between systems A and B.Comment: 11 pages, 7 figures. Accepted for the ApJS Special Issue on the Chandra Carina Complex Project (CCCP), scheduled for publication in May 2011. All 16 CCCP Special Issue papers are available at http://cochise.astro.psu.edu/Carina_public/special_issue.html through 2011 at leas

HIF-1 alpha-independent hypoxia-induced rapid PTK6 stabilization is associated with increased motility and invasion

© 2014 Landes Bioscience. PTK6/Brk is a non-receptor tyrosine kinase overexpressed in cancer. Here we demonstrate that cytosolic PTK6 is rapidly and robustly induced in response to hypoxic conditions in a HIF-1-independent manner. Furthermore, a proportion of hypoxic PTK6 subsequently re-localized to the cell membrane. We observed that the rapid stabilization of PTK6 is associated with a decrease in PTK6 ubiquitylation and we have identified c-Cbl as a putative PTK6 E3 ligase in normoxia. The consequences of hypoxia-induced PTK6 stabilization and subcellular re-localization to the plasma membrane include increased cell motility and invasion, suggesting PTK6 targeting as a therapeutic approach to reduce hypoxia-regulated metastatic potential. This could have particular significance for breast cancer patients with triple negative disease

Chandra/ACIS-I study of the X-ray properties of the NGC 6611 and M16 stellar population

Mechanisms regulating the origin of X-rays in YSOs and the correlation with their evolutionary stage are under debate. Studies of the X-ray properties in young clusters allow to understand these mechanisms. One ideal target for this analysis is the Eagle Nebula (M16), with its central cluster NGC6611. At 1750 pc from the Sun, it harbors 93 OB stars, together with a population of low-mass stars from embedded protostars to disk-less Class III objects, with age <= 3Myrs. We study an archival 78 ksec Chandra/ACIS-I observation of NGC6611, and two new 80ksec observations of the outer region of M16, one centered on the Column V, and one on a region of the molecular cloud with ongoing star-formation. We detect 1755 point sources, with 1183 candidate cluster members (219 disk-bearing and 964 disk-less). We study the global X-ray properties of M16 and compare them with those of the Orion Nebula Cluster. We also compare the level of X-ray emission of Class II and Class III stars, and analyze the X-ray spectral properties of OB stars. Our study supports the lower level of X-ray activity for the disk-bearing stars with respect to the disk-less members. The X-ray Luminosity Function (XLF) of M16 is similar to that of Orion, supporting the universality of the XLF in young clusters. 85% of the O stars of NGC6611 have been detected in X-rays. With only one possible exception, they show soft spectra with no hard component, indicating that mechanisms for the production of hard X-ray emission in O stars are not operating in NGC 6611.Comment: Accepted in Ap

The Spectra and Variability of X-ray Sources in a Deep Chandra Observation of the Galactic Center

We examine the X-ray spectra and variability of the sample of X-ray sources with L_X = 10^{31}-10^{33} erg s^{-1} identified within the inner 9' of the Galaxy. Very few of the sources exhibit intra-day or inter-month variations. We find that the spectra of the point sources near the Galactic center are very hard between 2--8 keV, even after accounting for absorption. When modeled as power laws the median photon index is Gamma=0.7, while when modeled as thermal plasma we can only obtain lower limits to the temperature of kT>8 keV. The combined spectra of the point sources is similarly hard, with a photon index of Gamma=0.8. Strong line emission is observed from low-ionization, He-like, and H-like Fe, both in the average spectra and in the brightest individual sources. The line ratios of the highly-ionized Fe in the average spectra are consistent with emission from a plasma in thermal equilibrium. This line emission is observed whether average spectra are examined as a function of the count rate from the source, or as a function of the hardness ratios of individual sources. This suggests that the hardness of the spectra may in fact to due local absorption that partially-covers the X-ray emitting regions in the Galactic center systems. We suggest that most of these sources are intermediate polars, which (1) often exhibit hard spectra with prominent Fe lines, (2) rarely exhibit either flares on short time scales or changes in their mean X-ray flux on long time scales, and (3) are the most numerous hard X-ray sources with comparable luminosities in the Galaxy.Comment: 27 pages, including 13 figures. To appear in ApJ, 1 October 2004, v613 issue. An electronic version of table 2 is on http://astro.ucla.edu/~mmuno/sgra/table2_electronic.txt and reduced data files for each source are available on http://www.astro.psu.edu/users/niel/galcen-xray-data/galcen-xray-data.htm

Global X-ray properties of the O and B stars in Carina

The key empirical property of the X-ray emission from O stars is a strong correlation between the bolometric and X-ray luminosities. In the framework of the Chandra Carina Complex Project, 129 O and B stars have been detected as X-ray sources; 78 of those, all with spectral type earlier than B3, have enough counts for at least a rough X-ray spectral characterization. This leads to an estimate of the Lx/Lbol ratio for an exceptional number of 60 O stars belonging to the same region and triples the number of Carina massive stars studied spectroscopically in X-rays. The derived log(Lx/Lbol) is -7.26 for single objects, with a dispersion of only 0.21dex. Using the properties of hot massive stars listed in the literature, we compare the X-ray luminosities of different types of objects. In the case of O stars, the Lx/Lbol ratios are similar for bright and faint objects, as well as for stars of different luminosity classes or spectral types. Binaries appear only slightly harder and slightly more luminous in X-rays than single objects; the differences are not formally significant (at the 1% level), except for the Lx/Lbol ratio in the medium (1.0--2.5keV) energy band. Weak-wind objects have similar X-ray luminosities but they display slightly softer spectra compared to "normal" O stars with the same bolometric luminosity. Discarding three overluminous objects, we find a very shallow trend of harder emission in brighter objects. The properties of the few B stars bright enough to yield some spectral information appear to be different overall (constant X-ray luminosities, harder spectra), hinting that another mechanism for producing X-rays, besides wind shocks, might be at work. However, it must be stressed that the earliest and X-ray brightest amongst these few detected objects are similar to the latest O stars, suggesting a possibly smooth transition between the two processes.Comment: 30 pages, 9 figures. Accepted for the ApJS Special Issue on the Chandra Carina Complex Project (CCCP), scheduled for publication in May 2011. All 16 CCCP Special Issue papers are available at http://cochise.astro.psu.edu/Carina_public/special_issue.html through 2011 at leas

What Happens after the First Shock? Temporal changes in microbial ecx-values in metal contaminated field trials

Extended abstract.Kris Broos, Michael Warne, Mike McLaughli