Evaluation of ten strains of Salmonella enteritidis in ricotta stored at 4 and 8 °C

Salmonella Enteritidis es uno los agentes etiológicos involucrados con mayor frecuencia en las infecciones alimentarias registradas en América del Sur. El objetivo de este trabajo fue evaluar diez cepas de Salmonella Enteritidis inoculadas en muestras de ricota e incubadas a 4 y 8°C durante 7 días. A las temperaturas estudiadas los recuentos de Salmonella Enteritidis se mantuvieron constantes durante el período de almacenamiento, lo que implicaría que las temperaturas de almacenamiento estudiadas controlan el desarrollo de este microorganismo.Salmonella Enteritidis is one of the more commonly pathogen involved in food- borne in South America. The aim of this work was evaluate ten Salmonella Enteritidis strains inoculated in ricotta samples, stored at 4 and 8°C for 7 days. Counts of Salmonella Enteritidis were constant during the store period, which would imply that the storage temperatures studied to control development of this organism.Facultad de Ciencias Veterinaria

Evaluation of ten strains of Salmonella enteritidis in ricotta stored at 4 and 8 °C

Salmonella Enteritidis es uno los agentes etiológicos involucrados con mayor frecuencia en las infecciones alimentarias registradas en América del Sur. El objetivo de este trabajo fue evaluar diez cepas de Salmonella Enteritidis inoculadas en muestras de ricota e incubadas a 4 y 8°C durante 7 días. A las temperaturas estudiadas los recuentos de Salmonella Enteritidis se mantuvieron constantes durante el período de almacenamiento, lo que implicaría que las temperaturas de almacenamiento estudiadas controlan el desarrollo de este microorganismo.Salmonella Enteritidis is one of the more commonly pathogen involved in food- borne in South America. The aim of this work was evaluate ten Salmonella Enteritidis strains inoculated in ricotta samples, stored at 4 and 8°C for 7 days. Counts of Salmonella Enteritidis were constant during the store period, which would imply that the storage temperatures studied to control development of this organism.Facultad de Ciencias Veterinaria

Evaluation of ten strains of Salmonella enteritidis in ricotta stored at 4 and 8 °C

Salmonella Enteritidis es uno los agentes etiológicos involucrados con mayor frecuencia en las infecciones alimentarias registradas en América del Sur. El objetivo de este trabajo fue evaluar diez cepas de Salmonella Enteritidis inoculadas en muestras de ricota e incubadas a 4 y 8°C durante 7 días. A las temperaturas estudiadas los recuentos de Salmonella Enteritidis se mantuvieron constantes durante el período de almacenamiento, lo que implicaría que las temperaturas de almacenamiento estudiadas controlan el desarrollo de este microorganismo.Salmonella Enteritidis is one of the more commonly pathogen involved in food- borne in South America. The aim of this work was evaluate ten Salmonella Enteritidis strains inoculated in ricotta samples, stored at 4 and 8°C for 7 days. Counts of Salmonella Enteritidis were constant during the store period, which would imply that the storage temperatures studied to control development of this organism.Facultad de Ciencias Veterinaria

Analysis of a panel of antibodies to APC reveals consistent activity towards an unidentified protein

Acquisition of truncating mutations in the adenomatous polyposis coli (APC) protein underlies the progression of the majority of sporadic and familial colorectal cancers. As such, the localisation patterns and interacting partners of APC have been extensively studied in a range of systems, relying on the use of a broad panel of antibodies. Until recently, antibodies to APC have been used largely unchecked. However, several recent reports have been invaluable in clarifying the use of a number of antibodies commonly used to detect APC. Here, we analyse the specificity of a further subset of antibodies to APC. We used a panel of six commercially available antibodies (directed to the amino and carboxy termini of APC) and confirm the detection of full-length APC by immunoblotting. We demonstrate that a 150 kDa protein, also reproducibly detected by this panel of antibodies, is unlikely to be APC. We present data for the immunological staining patterns of the APC antibodies and validate the results through RNAi. Using this approach, we confirm that the apical staining pattern, observed by immunofluorescence and previously reported in cell systems, is unlikely to be APC. Finally, we present our data as a summary of APC-antibody specificities for APC

Rho GTPase Cdc42 Is a Direct Interacting Partner of Adenomatous Polyposis Coli Protein and Can Alter Its Cellular Localization

Adenomatous Polyposis Coli (APC) is a tumor suppressor gene product involved in colon cancer. APC is a large multidomain molecule of 2843 amino acid residues and connects cell-cell adhesion, the F-actin/microtubule cytoskeleton and the nucleus. Here we show that Cdc42 interacts directly with the first three armadillo repeats of APC by yeast two-hybrid screens. We confirm the Cdc42-APC interaction using pulldown assays in vitro and FRET assays in vivo. Interestingly, Cdc42 interacts with APC at leading edge sites where F-actin is enriched. In contrast, Cdc42 interacts with the truncated mutant APC1–1638 in cellular puncta associated with the golgi-lysozome pathway in transfected CHO cells. In HCT116 and SW480 cells, Cdc42 induces the relocalization of endogenous APC and the mutant APC1–1338 to the plasma membrane and cellular puncta, respectively. Taken together, these data indicate that the Cdc42-APC interaction induces localization of both APC and mutant APC and may thus play a direct role in the functions of these proteins

Adenomatous Polyposis Coli Regulates Axon Arborization and Cytoskeleton Organization via Its N-Terminus

Conditional deletion of APC leads to marked disruption of cortical development and to excessive axonal branching of cortical neurons. However, little is known about the cell biological basis of this neuronal morphological regulation. Here we show that APC deficient cortical neuronal growth cones exhibit marked disruption of both microtubule and actin cytoskeleton. Functional analysis of the different APC domains revealed that axonal branches do not result from stabilized β-catenin, and that the C-terminus of APC containing microtubule regulatory domains only partially rescues the branching phenotype. Surprisingly, the N-terminus of APC containing the oligomerization domain and the armadillo repeats completely rescues the branching and cytoskeletal abnormalities. Our data indicate that APC is required for appropriate axon morphological development and that the N-terminus of APC is important for regulation of the neuronal cytoskeleton

NEOTROPICAL XENARTHRANS: a data set of occurrence of xenarthran species in the Neotropics

Xenarthrans – anteaters, sloths, and armadillos – have essential functions for ecosystem maintenance, such as insect control and nutrient cycling, playing key roles as ecosystem engineers. Because of habitat loss and fragmentation, hunting pressure, and conflicts with 24 domestic dogs, these species have been threatened locally, regionally, or even across their full distribution ranges. The Neotropics harbor 21 species of armadillos, ten anteaters, and six sloths. Our dataset includes the families Chlamyphoridae (13), Dasypodidae (7), Myrmecophagidae (3), Bradypodidae (4), and Megalonychidae (2). We have no occurrence data on Dasypus pilosus (Dasypodidae). Regarding Cyclopedidae, until recently, only one species was recognized, but new genetic studies have revealed that the group is represented by seven species. In this data-paper, we compiled a total of 42,528 records of 31 species, represented by occurrence and quantitative data, totaling 24,847 unique georeferenced records. The geographic range is from the south of the USA, Mexico, and Caribbean countries at the northern portion of the Neotropics, to its austral distribution in Argentina, Paraguay, Chile, and Uruguay. Regarding anteaters, Myrmecophaga tridactyla has the most records (n=5,941), and Cyclopes sp. has the fewest (n=240). The armadillo species with the most data is Dasypus novemcinctus (n=11,588), and the least recorded for Calyptophractus retusus (n=33). With regards to sloth species, Bradypus variegatus has the most records (n=962), and Bradypus pygmaeus has the fewest (n=12). Our main objective with Neotropical Xenarthrans is to make occurrence and quantitative data available to facilitate more ecological research, particularly if we integrate the xenarthran data with other datasets of Neotropical Series which will become available very soon (i.e. Neotropical Carnivores, Neotropical Invasive Mammals, and Neotropical Hunters and Dogs). Therefore, studies on trophic cascades, hunting pressure, habitat loss, fragmentation effects, species invasion, and climate change effects will be possible with the Neotropical Xenarthrans dataset