2,094 research outputs found
Aerothermodynamic Assessment of Corrugated Panel Thermal Protection Systems
The feasibility of using corrugated panels as a thermal protection system for an advanced space transportation vehicle was investigated. The study consisted of two major tasks: development of improved correlations for wind tunnel heat transfer and pressure data to yield design techniques, and application of the design techniques to determine if corrugated panels have application future aerospace vehicles. A single-stage-to-orbit vehicle was used to assess advantages and aerothermodynamic penalties associated with use of such panels. In the correlation task, experimental turbulent heat transfer and pressure data obtained on corrugation roughened surfaces during wind tunnel testing were analyzed and compared with flat plate data. The correlations and data comparisons included the effects of a large range of geometric, inviscid flow, internal boundary layer, and bulk boundary layer parameters in supersonic and hypersonic flow
High-Resolution Analysis of the Efficiency, Heritability, and Editing Outcomes of CRISPR/Cas9-Induced Modifications of NCED4 in Lettuce (Lactuca sativa).
CRISPR/Cas9 is a transformative tool for making targeted genetic alterations. In plants, high mutation efficiencies have been reported in primary transformants. However, many of the mutations analyzed were somatic and therefore not heritable. To provide more insights into the efficiency of creating stable homozygous mutants using CRISPR/Cas9, we targeted LsNCED4 (9-cis-EPOXYCAROTENOID DIOXYGENASE4), a gene conditioning thermoinhibition of seed germination in lettuce. Three constructs, each capable of expressing Cas9 and a single gRNA targeting different sites in LsNCED4, were stably transformed into lettuce (Lactuca sativa) cvs. Salinas and Cobham Green. Analysis of 47 primary transformants (T1) and 368 T2 plants by deep amplicon sequencing revealed that 57% of T1 plants contained events at the target site: 28% of plants had germline mutations in one allele indicative of an early editing event (mono-allelic), 8% of plants had germline mutations in both alleles indicative of two early editing events (bi-allelic), and the remaining 21% of plants had multiple low frequency mutations indicative of late events (chimeric plants). Editing efficiency was similar in both genotypes, while the different gRNAs varied in efficiency. Amplicon sequencing of 20 T1 and more than 100 T2 plants for each of the three gRNAs showed that repair outcomes were not random, but reproducible and characteristic for each gRNA. Knockouts of NCED4 resulted in large increases in the maximum temperature for seed germination, with seeds of both cultivars capable of germinating >70% at 37°. Knockouts of NCED4 provide a whole-plant selectable phenotype that has minimal pleiotropic consequences. Targeting NCED4 in a co-editing strategy could therefore be used to enrich for germline-edited events simply by germinating seeds at high temperature
Lymphotoxins and cytomegalovirus cooperatively induce interferon-beta, establishing host-virus détente
Tumor necrosis factor (TNF)-related cytokines regulate cell death and survival and provide strong selective pressures for viruses, such as cytomegalovirus (CMV), to evolve counterstrategies in order to persist in immune-competent hosts. Signaling by the lymphotoxin (LT)-β receptor or TNF receptor-1, but not Fas or TRAIL receptors, inhibits the cytopathicity and replication of human CMV by a nonapoptotic, reversible process that requires nuclear factor κB (NF-κB)-dependent induction of interferon-β (IFN-β). Efficient induction of IFN-β requires virus infection and LT signaling, demonstrating the need for both host and viral factors in the curtailment of viral replication without cellular elimination. LTα-deficient mice and LTβR-Fc transgenic mice were profoundly susceptible to murine CMV infection. Together, these results reveal an essential and conserved role for LTs in establishing host defense to CMV
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Identity and function of an essential nitrogen ligand of the nitrogenase cofactor biosynthesis protein NifB.
NifB is a radical S-adenosyl-L-methionine (SAM) enzyme that is essential for nitrogenase cofactor assembly. Previously, a nitrogen ligand was shown to be involved in coupling a pair of [Fe4S4] clusters (designated K1 and K2) concomitant with carbide insertion into an [Fe8S9C] cofactor core (designated L) on NifB. However, the identity and function of this ligand remain elusive. Here, we use combined mutagenesis and pulse electron paramagnetic resonance analyses to establish histidine-43 of Methanosarcina acetivorans NifB (MaNifB) as the nitrogen ligand for K1. Biochemical and continuous wave electron paramagnetic resonance data demonstrate the inability of MaNifB to serve as a source for cofactor maturation upon substitution of histidine-43 with alanine; whereas x-ray absorption spectroscopy/extended x-ray fine structure experiments further suggest formation of an intermediate that lacks the cofactor core arrangement in this MaNifB variant. These results point to dual functions of histidine-43 in structurally assisting the proper coupling between K1 and K2 and concurrently facilitating carbide formation via deprotonation of the initial carbon radical
3D-Laser-Scanning Technique Applied to Bulk Density Measurements of Apollo Lunar Samples
In order to better interpret gravimetric data from orbiters such as GRAIL and LRO to understand the subsurface composition and structure of the lunar crust, it is import to have a reliable database of the density and porosity of lunar materials. To this end, we have been surveying these physical properties in both lunar meteorites and Apollo lunar samples. To measure porosity, both grain density and bulk density are required. For bulk density, our group has historically utilized sub-mm bead immersion techniques extensively, though several factors have made this technique problematic for our work with Apollo samples. Samples allocated for measurement are often smaller than optimal for the technique, leading to large error bars. Also, for some samples we were required to use pure alumina beads instead of our usual glass beads. The alumina beads were subject to undesirable static effects, producing unreliable results. Other investigators have tested the use of 3d laser scanners on meteorites for measuring bulk volumes. Early work, though promising, was plagued with difficulties including poor response on dark or reflective surfaces, difficulty reproducing sharp edges, and large processing time for producing shape models. Due to progress in technology, however, laser scanners have improved considerably in recent years. We tested this technique on 27 lunar samples in the Apollo collection using a scanner at NASA Johnson Space Center. We found it to be reliable and more precise than beads, with the added benefit that it involves no direct contact with the sample, enabling the study of particularly friable samples for which bead immersion is not possibl
Novel heavy-traffic regimes for large-scale service systems
We introduce a family of heavy-traffic regimes for large scale service
systems, presenting a range of scalings that include both moderate and extreme
heavy traffic, as compared to classical heavy traffic. The heavy-traffic
regimes can be translated into capacity sizing rules that lead to
Economies-of-Scales, so that the system utilization approaches 100% while
congestion remains limited. We obtain heavy-traffic approximations for
stationary performance measures in terms of asymptotic expansions, using a
non-standard saddle point method, tailored to the specific form of integral
expressions for the performance measures, in combination with the heavy-traffic
regimes
The All-Sky Automated Survey for Supernovae (ASAS-SN) Light Curve Server v1.0
The All-Sky Automated Survey for Supernovae (ASAS-SN) is working towards
imaging the entire visible sky every night to a depth of V~17 mag. The present
data covers the sky and spans ~2-5~years with ~100-400 epochs of observation.
The data should contain some ~1 million variable sources, and the ultimate goal
is to have a database of these observations publicly accessible. We describe
here a first step, a simple but unprecedented web interface
https://asas-sn.osu.edu/ that provides an up to date aperture photometry light
curve for any user-selected sky coordinate. Because the light curves are
produced in real time, this web tool is relatively slow and can only be used
for small samples of objects. However, it also imposes no selection bias on the
part of the ASAS-SN team, allowing the user to obtain a light curve for any
point on the celestial sphere. We present the tool, describe its capabilities,
limitations, and known issues, and provide a few illustrative examples.Comment: 8 pages, 9 figures, submitted to PAS
H-2D control of recovery from Friend virus leukemia: H-2D region influences the kinetics of the T lymphocyte response to Friend virus.
A dynamical model of surrogate reactions
A new dynamical model is developed to describe the whole process of surrogate
reactions; transfer of several nucleons at an initial stage, thermal
equilibration of residues leading to washing out of shell effects and decay of
populated compound nuclei are treated in a unified framework. Multi-dimensional
Langevin equations are employed to describe time-evolution of collective
coordinates with a time-dependent potential energy surface corresponding to
different stages of surrogate reactions. The new model is capable of
calculating spin distributions of the compound nuclei, one of the most
important quantity in the surrogate technique. Furthermore, various observables
of surrogate reactions can be calculated, e.g., energy and angular distribution
of ejectile, and mass distributions of fission fragments. These features are
important to assess validity of the proposed model itself, to understand
mechanisms of the surrogate reactions and to determine unknown parameters of
the model. It is found that spin distributions of compound nuclei produced in
O+U O+U and O+U
O+U reactions are equivalent and much less than
10, therefore satisfy conditions proposed by Chiba and Iwamoto (PRC 81,
044604(2010)) if they are used as a pair in the surrogate ratio method.Comment: 17 pages, 5 figure
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