Collapse of Kaluza-Klein Bubbles

Kaluza-Klein theory admits ``bubble" configurations, in which the circumference of the fifth dimension shrinks to zero on some compact surface. A three parameter family of such bubble initial data at a moment of time-symmetry (some including a magnetic field) has been found by Brill and Horowitz, generalizing the (zero-energy) ``Witten bubble" solution. Some of these data have negative total energy. We show here that all the negative energy bubble solutions start out expanding away from the moment of time symmetry, while the positive energy bubbles can start out either expanding or contracting. Thus it is unlikely that the negative energy bubbles would collapse and produce a naked singularity.Comment: 6 pages, plain LaTeX, UMDGR-94-08

GEMPAK5 user's guide, version 5.0

GEMPAK is a general meteorological software package used to analyze and display conventional meteorological data as well as satellite derived parameters. The User's Guide describes the GEMPAK5 programs and input parameters and details the algorithms used for the meteorological computations

Elastic properties of (N(CH3)3H) (I) (TCNQ)

Journal ArticleWe present the results of a study of the temperature dependence of the Young's modulus and internal friction of (N(CH3 )3H)(I)(TCNQ). The Young's modulus was measured in a direction parallel to the TCNQ (tetracyanoquinodimethane) stacking axis using a vibrating reed technique. The results confirmed a transition observed via transport measurements at 150 K and predicted a transition (later observed) at 89 K. The data support the model that this system is a semiconductor at temperatures above 150 K despite the "metal-like" dc conductivity

Purification of the yeast Slx5–Slx8 protein complex and characterization of its DNA-binding activity

SLX5 and SLX8 encode RING-finger proteins that were previously identified based on their requirement for viability in yeast cells lacking Sgs1 DNA helicase. Slx5 and Slx8 proteins are known to be required for genome stability and to physically interact in yeast extracts; however, their biochemical functions are unknown. To address this question we purified and characterized recombinant Slx5 and Slx8 proteins. Here we show that Slx5 and Slx8 form a heterodimeric complex with double-stranded DNA (dsDNA)-binding activity. Individually, only the Slx8 subunit displays this activity. Structure–function studies indicate that the DNA-binding activity requires only the N-terminal 160 amino acids of Slx8, but not its C-terminal RING-finger domain. Alleles of SLX8 that express the RING-finger domain alone show almost complete complementation in yeast indicating that this DNA-binding domain is not essential for this in vivo function. Consistent with these findings we show that Slx5 immunolocalizes to the nucleus and that a portion of the Slx8 protein co-fractionates with chromatin. These results suggest that Slx5–Slx8 may act directly on DNA to promote genome stability

The Excess Method: A Multiwinner Approval Voting Procedure to Allocate Wasted Votes

In using approval voting to elect multiple winners to a committee or council, it is desirable that excess votes—approvals beyond those that a candidate needs to win a seat—not be wasted. The excess method does this by sequentially allocating excess votes to a voter’s as-yet-unelected approved candidates, based on the Jefferson method of apportionment. It is monotonic—approving of a candidate never hurts and may help him or her get elected—computationally easy, and less manipulable than related methods. In parliamentary systems with party lists, the excess method is equivalent to the Jefferson method and thus ensures the approximate proportional representation of political parties. As a method for achieving proportional representation (PR) on a committee or council, we compare it to other PR methods proposed by Hare, Andrae, and Droop for preferential voting systems, and by Phragmén for approval voting. Because voters can vote for multiple candidates or parties, the excess method is likely to abet coalitions that cross ideological and party lines and to foster greater consensus in voting bodies

The Excess Method: A Multiwinner Approval Voting Procedure to Allocate Wasted Votes

In using approval voting to elect multiple winners to a committee or council, it is desirable that excess votes—approvals beyond those that a candidate needs to win a seat—not be wasted. The excess method does this by sequentially allocating excess votes to a voter’s as-yet-unelected approved candidates, based on the Jefferson method of apportionment. It is monotonic—approving of a candidate never hurts and may help him or her get elected—computationally easy, and less manipulable than related methods. In parliamentary systems with party lists, the excess method is equivalent to the Jefferson method and thus ensures the approximate proportional representation of political parties. As a method for achieving proportional representation (PR) on a committee or council, we compare it to other PR methods proposed by Hare, Andrae, and Droop for preferential voting systems, and by Phragmén for approval voting. Because voters can vote for multiple candidates or parties, the excess method is likely to abet coalitions that cross ideological and party lines and to foster greater consensus in voting bodies

Themed Issue Article: Conservation Physiology of Marine Fishes Fisheries conservation on the high seas: linking conservation physiology and fisheries ecology for the management of large pelagic fishes

Populations of tunas, billfishes and pelagic sharks are fished at or over capacity in many regions of the world. They are captured by directed commercial and recreational fisheries (the latter of which often promote catch and release) or as incidental catch or bycatch in commercial fisheries. Population assessments of pelagic fishes typically incorporate catch-per-unit-effort time-series data from commercial and recreational fisheries; however, there have been notable changes in target species, areas fished and depth-specific gear deployments over the years that may have affected catchability. Some regional fisheries management organizations take into account the effects of time-and area-specific changes in the behaviours of fish and fishers, as well as fishing gear, to standardize catch-per-unit-effort indices and refine population estimates. However, estimates of changes in stock size over time may be very sensitive to underlying assumptions of the effects of oceanographic conditions and prey distribution on the horizontal and vertical movement patterns and distribution of pelagic fishes. Effective management and successful conservation of pelagic fishes requires a mechanistic understanding of their physiological and behavioural responses to environmental variability, potential for interaction with commercial and recreational fishing gear, and the capture process. The interdisciplinary field of conservation physiology can provide insights into pelagic fish demography and ecology (including environmental relationships and interspecific interactions) by uniting the complementary expertise and skills of fish physiologists and fisheries scientists. The iterative testing by one discipline of hypotheses generated by the other can span the fundamental-applied science continuum, leading to the development of robust insights supporting informed management. The resulting species-specific understanding of physiological abilities and tolerances can help to improve stock assessments, develop effective bycatch-reduction strategies, predict rates of post-release mortality, and forecast the population effects of environmental change. In this synthesis, we review several examples of these interdisciplinary collaborations that currently benefit pelagic fisheries management

GEMPAK5. Part 1: GEMPAK5 programmer's guide, version 5.0

GEMPAK is a general meteorological software package used to analyze and display conventional meteorological data as well as satellite derived parameters. The Programmer's Guide describes the subroutines which can be used to build new GEMPAK programs. Part 1 contains GEMPAK subroutines

Role of immediate reconstruction for elderly breast cancer patients

With rising numbers of elderly women developing breast cancer, treatment options must evolve which take into consideration quality of life and functional status. Although fewer women at all ages are undergoing mastectomy, for those elderly women who undergo mastectomy, reconstruction options should be offered. Over the past 5 years, increasing numbers of elderly women are electing immediate reconstruction (IM)

Resolution by Unassisted Top3 Points to Template Switch Recombination Intermediates during DNA Replication

The evolutionarily conserved Sgs1/Top3/Rmi1 (STR) complex plays vital roles in DNA replication and repair. One crucial activity of the complex is dissolution of toxic X-shaped recombination intermediates that accumulate during replication of damaged DNA. However, despite several years of study the nature of these X-shaped molecules remains debated. Here we use genetic approaches and two-dimensional gel electrophoresis of genomic DNA to show that Top3, unassisted by Sgs1 and Rmi1, has modest capacities to provide resistance to MMS and to resolve recombination-dependent X-shaped molecules. The X-shaped molecules have structural properties consistent with hemicatenane-related template switch recombination intermediates (Rec-Xs) but not Holliday junction (HJ) intermediates. Consistent with these findings, we demonstrate that purified Top3 can resolve a synthetic Rec-X but not a synthetic double HJ in vitro. We also find that unassisted Top3 does not affect crossing over during double strand break repair, which is known to involve double HJ intermediates, confirming that unassisted Top3 activities are restricted to substrates that are distinct from HJs. These data help illuminate the nature of the X-shaped molecules that accumulate during replication of damaged DNA templates, and also clarify the roles played by Top3 and the STR complex as a whole during the resolution of replication-associated recombination intermediates