Human Neural Cells Transiently Express Reelin during Olfactory Placode Development.

Reelin, an extracellular glycoprotein is essential for migration and correct positioning of neurons during development. Since the olfactory system is known as a source of various migrating neuronal cells, we studied Reelin expression in the two chemosensory olfactory systems, main and accessory, during early developmental stages of human foetuses/embryos from Carnegie Stage (CS) 15 to gestational week (GW) 14. From CS 15 to CS 18, but not at later stages, a transient expression of Reelin was detected first in the presumptive olfactory and then in the presumptive vomeronasal epithelium. During the same period, Reelin-positive cells detach from the olfactory/vomeronasal epithelium and migrate through the mesenchyme beneath the telencephalon. Dab 1, an adaptor protein of the Reelin pathway, was simultaneously expressed in the migratory mass from CS16 to CS17 and, at later stages, in the presumptive olfactory ensheathing cells. Possible involvements of Reelin and Dab 1 in the peripheral migrating stream are discussed.journal articleresearch support, non-u.s. gov't20152015 08 13importe

Loss of STOP Protein Impairs Peripheral Olfactory Neurogenesis

International audienceIn conclusion, STOP protein seems to be involved in the establishment of synapses in the olfactory glomerulus. Our results indicate that the olfactory system of STOP null mice is a well-suited experimental model (1) for the study of the mechanism of action of STOP protein in synaptic function/plasticity and (2) for pathophysiological studies of the mechanisms of altered neuronal connections in schizophrenia

Evaluation de la prise en charge de la douleur chronique au Centre anti-douleur de l'Hôtel-Dieu (étude sur 103 patients suivis sur 2 ans)

PARIS-BIUP (751062107) / SudocSudocFranceF

Fibrinogen concentrates for post-partum haemorrhage? Do not miss the most relevant population!

SCOPUS: ed.jinfo:eu-repo/semantics/publishe

The place of fibrinogen concentrates in the management of perioperative bleeding: A position paper from the Francophone Working Group on Perioperative Haemostasis (GIHP)

The consumption of fibrinogen concentrates has been increasing steadily for several years in surgery, trauma and obstetrics. However, data from the literature are conflicting. The French Working Group on Perioperative Haemostasis (GIHP) proposes a position paper based on a narrative review of the literature, and addresses the following questions: What is the exact role of fibrinogen in haemostasis? Which rational support for the use of perioperative fibrinogen? Which thrombotic risk? What are the most recent professional recommendations on the use of fibrinogen concentrates? Then, evidence-based recommendations are proposed: 1) it is suggested not to administer prophylactic FC to prevent haemorrhage; 2) it is suggested not to use FC alone. Haemostatic treatment must be comprehensive, include other haemostatic treatments and must be limited in cases of severe active haemorrhage; 3) the GIHP suggests urgent measurement of fibrinogen plasma concentration in a biology laboratory or functional fibrinogen by viscoelastic methods. The choice between the two methods must be guided by the time to receive the results from a certified organisation with, in particular, authorisation to perform delocalised biologic examinations; 4) it is suggested not to administer FC when the fibrinogen concentration is superior to 1.5 g/L or when there is a functional fibrinogen deficit (with the possible exception in obstetrics where the threshold could be 2.0 g/L); 5) if FC are administered, an initial dose of 25–50 mg/kg is proposed.SCOPUS: re.jinfo:eu-repo/semantics/publishe

Reelin expression in the telencephalon and in the olfactory bulb (OB).

<p>(A, B) Same case as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0135710#pone.0135710.g001" target="_blank">Fig 1A</a>. Beta-III tubulin- and Reelin-positive cells in the marginal zone of the telencephalon when the olfactory placode has invaginated in front of the telencephalon. (C) A thicker layer of Reelin-positive cells as during the preceding stage is present in the external part of the marginal zone. (D, E) The OB is well developed and shows a thick, beta-III tubulin-positive olfactory fibre layer. (F) The weakly Reelin-positive mitral cells in the OB contrast with the strongly Reelin-positive Cajal Retzius cells. (G) High magnification of the dorsal cortex showing Reelin mRNA in the single layer of Cajal-Retzius cells precursors. (H, I) Nearly adjacent sections comparing Reelin-positive cells by <i>in situ</i> hybridization (H) or immunocytochemistry (I). (J-L) At GW 12, the mitral cell layer is well delineated, including calretinin-negative but Reelin-positive cells. Abbreviations: (cal) calretinin immunostaining, (CR) Cajal-Retzius cells, (CS) Carnegie stage, (GW) gestational week, (HE) hematoxylin-eosin staining, (MC) mitral cell layer, (OB) olfactory bulb, (OF) olfactory fibre layer, (tub) beta-III tubuline immunostaining.</p

Development of the human olfactory system.

<p>(A-F) Development of the OE and VNE. (A) The arrow points to the invaginated OP in front of the telencephalon. (B) The nasal cavities have developed and the presumptive VNO is first delineated (arrows). (C) A transverse section of a VNO shows a small tube lined with a pseudostratified epithelium. (D, E) The entry of the VNO (arrow) is lined with a respiratory epithelium; calretinin-positive cells are present in the VNE and in the cell-stream leaving the VNO. (F) Many calretinin-positive cells are present in the OE. (G-I) Development of the OB. (G) Higher magnification of A shows the basal telencephalon consists of a thick proliferating ventricular zone and a narrow marginal zone. (H) ICBP immunostaining (purple nuclei) shows that the MZ has developed. (I) A low magnification of a coronal section at CS 20 shows that the olfactory fibres have reached the telencephalon and form a distinct layer at the surface of the developing OB; OBs are present with a peripheral fibre layer, a differentiating layer and a ventricular layer. Abbreviations: (cal) calretinin immunostaining, (CS) Carnegie stage, (HE) hematoxylin-eosin staining, (MZ) marginal zone, (OB) olfactory bulb, (OP) olfactory placode, (Tel) telencephalon.</p

Dab 1 expression in the olfactory nerve.

<p>(A-C): Near adjacent sections of the OB at CS 20. (A) Calretinin–positive fibres form a well delineated olfactory fibre layer around the protruding OB after the olfactory fibres have reached the ventricule (arrow). (B) Weak Reelin-staining of the developing mitral cells. (C) Dab 1 expression by cells in the olfactory fibre layer. (D, E) Adjacent sections of the olfactory lamina propria at GW12: Dab 1 and S100 protein expression show a similar pattern in the olfactory fascicles. Abbreviations: (cal) calretinin immunostaining, (CS) Carnegie stage, (GW) gestational week, (OF) olfactory fibre layer.</p