Efficient cellular automata for 2D / 3D free-form modeling

This paper presents an approach for efficiently simulating highly deformable 2D substances undergoing viscoplastic deformations in real time. The user deals with objects in the same way as clay works. Based on the work of Y. Takai and H. Arata, we suggest a new approach for the computation of repartition rules. We use a discrete 2D space in which each pixel is given a certain amount of clay at the intialisation of the system. The user’s tool only moves some clay from a pixel to another, creating an overload. The repartition of this overload amoung the neighbours is then made according to the laws of plastic deformation thanks to a cellular automaton

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form