Gender, class and space in the field of parenthood comparing middle-class fractions in Amsterdam and London

This paper argues that becoming a parent/carer can be seen as a new field of social relations and suggests how gender is the key mechanism in the reconfiguration of class relations in this field. By conceptualising parenthood as a field, that is a social world with specific stakes and rules, this study suggests that residential decisions and strategies developed by different middle-class households do not solely depend on their class habitus, but also on gendered positions and dispositions in respect to division of labour, child care and school choice. Drawing on interview data from London and Amsterdam, this study re-addresses the issue of middle–class time-space trajectories at a specific period in the life course. We contend that the middle classes are not just differentiated by various orientations of capital (economic versus cultural) but that interaction of class and gender is also key for understanding practices of the middle classes as they enter the field of parenthood. These practices are strongly influenced by labour market and welfare regimes (as the Netherlands/England comparison makes clear). In the new field of parenthood the work of realigning class habitus (through social reproduction) is highly gendered, but to different degrees that are made evident in the different neighbourhood settings. In terms of urban space this points to the significance of the particular neighbourhood structure and opportunities of the city as a whole as well as a more active idea of the role of space in the particular working out of class and gender in specific neighbourhood contexts. Urban space is a situating framework and an active process in trajectories of social reproduction

The transition to parenthood in urban space: continuity and disruption of embodied experience and spatial practice.

This paper explores the impacts of the transition to parenthood on the relationship between (from a Bourdieusian perspective) habitus and field in social reproduction. The paper is based on a longitudinal study of white middle-class gentrifier households in Amsterdam before and after childbirth. The study reveals the disruptive (as well as re-configurative) impact of parenthood as transition, even for this relatively privileged group (in terms of legitimated habitus and spatial discretion and mobility). Our findings strongly suggest the highly gendered nature of this transition, and also reveal the significance of spatial dimensions of this gendered experience – from embodiment all the way through to changing relationships in urban space. These impacts suggest parenthood as a distinct ‘field’ of social struggle and emphasise the significance of gender and spatial experience in understanding habitus-field relations, alongside the more acknowledged temporalities of social reproduction

Variation in pre-PCR processing of FFPE samples leads to discrepancies in BRAF and EGFR mutation detection: a diagnostic RING trial.

Aims Mutation detection accuracy has been described extensively; however, it is surprising that pre-PCR processing of formalin-fixed paraffin-embedded (FFPE) samples has not been systematically assessed in clinical context. We designed a RING trial to (i) investigate pre-PCR variability, (ii) correlate pre-PCR variation with EGFR/BRAF mutation testing accuracy and (iii) investigate causes for observed variation. Methods 13 molecular pathology laboratories were recruited. 104 blinded FFPE curls including engineered FFPE curls, cell-negative FFPE curls and control FFPE tissue samples were distributed to participants for pre-PCR processing and mutation detection. Follow-up analysis was performed to assess sample purity, DNA integrity and DNA quantitation. Results Rate of mutation detection failure was 11.9%. Of these failures, 80% were attributed to pre-PCR error. Significant differences in DNA yields across all samples were seen using analysis of variance (p<0.0001), and yield variation from engineered samples was not significant (p=0.3782). Two laboratories failed DNA extraction from samples that may be attributed to operator error. DNA extraction protocols themselves were not found to contribute significant variation. 10/13 labs reported yields averaging 235.8ng (95% CI 90.7 to 380.9) from cell-negative samples, which was attributed to issues with spectrophotometry. DNA measurements using Qubit Fluorometry demonstrated a median fivefold overestimation of DNA quantity by Nanodrop Spectrophotometry. DNA integrity and PCR inhibition were factors not found to contribute significant variation. Conclusions In this study, we provide evidence demonstrating that variation in pre-PCR steps is prevalent and may detrimentally affect the patient's ability to receive critical therapy. We provide recommendations for preanalytical workflow optimisation that may reduce errors in down-stream sequencing and for next-generation sequencing library generation

Spectral Energy Distribution Fitting of Hetdex Pilot Survey Ly-alpha Emitters in Cosmos and Goods-N

We use broadband photometry extending from the rest-frame UV to the near-IR to fit the individual spectral energy distributions of 63 bright (L(Ly-alpha) greater than 10(exp 43) erg s(exp 1) Ly-alpha emitting galaxies (LAEs) in the redshift range 1.9 less than z less than 3.6. We find that these LAEs are quite heterogeneous, with stellar masses that span over three orders of magnitude, from 7.5 greater than logM/solar mass less than 10.5. Moreover, although most LAEs have small amounts of extinction, some high-mass objects have stellar reddenings as large as E(B V ) is approximately 0.4. Interestingly, in dusty objects the optical depths for Ly-alpha and the UV continuum are always similar, indicating that Ly photons are not undergoing many scatters before escaping their galaxy. In contrast, the ratio of optical depths in low-reddening systems can vary widely, illustrating the diverse nature of the systems. Finally, we show that in the star-formation-rate-log-mass diagram, our LAEs fall above the "main-sequence" defined by z is approximately 3 continuum selected star-forming galaxies. In this respect, they are similar to submillimeter-selected galaxies, although most LAEs have much lower mass

Genetic effects on gene expression across human tissues

Characterization of the molecular function of the human genome and its variation across individuals is essential for identifying the cellular mechanisms that underlie human genetic traits and diseases. The Genotype-Tissue Expression (GTEx) project aims to characterize variation in gene expression levels across individuals and diverse tissues of the human body, many of which are not easily accessible. Here we describe genetic effects on gene expression levels across 44 human tissues. We find that local genetic variation affects gene expression levels for the majority of genes, and we further identify inter-chromosomal genetic effects for 93 genes and 112 loci. On the basis of the identified genetic effects, we characterize patterns of tissue specificity, compare local and distal effects, and evaluate the functional properties of the genetic effects. We also demonstrate that multi-tissue, multi-individual data can be used to identify genes and pathways affected by human disease-associated variation, enabling a mechanistic interpretation of gene regulation and the genetic basis of diseas

Genetic effects on gene expression across human tissues

Characterization of the molecular function of the human genome and its variation across individuals is essential for identifying the cellular mechanisms that underlie human genetic traits and diseases. The Genotype-Tissue Expression (GTEx) project aims to characterize variation in gene expression levels across individuals and diverse tissues of the human body, many of which are not easily accessible. Here we describe genetic effects on gene expression levels across 44 human tissues. We find that local genetic variation affects gene expression levels for the majority of genes, and we further identify inter-chromosomal genetic effects for 93 genes and 112 loci. On the basis of the identified genetic effects, we characterize patterns of tissue specificity, compare local and distal effects, and evaluate the functional properties of the genetic effects. We also demonstrate that multi-tissue, multi-individual data can be used to identify genes and pathways affected by human disease-associated variation, enabling a mechanistic interpretation of gene regulation and the genetic basis of disease

The Determinants of educational outcomes : The impact of families, peers, teachers and schools.

xvii, 355 p.; 20 cm